Difference between revisions of "Team:Valencia UPV/Improve"

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The BioBrick chosen to be improved was the YFP coding sequence: <a href=http://parts.igem.org/Part:BBa_K592101> BBa_K592101</a>. To do this, we first standardized the part to be compatible with the <a href=https://2018.igem.org/Team:Valencia_UPV/GB3>  GoldenBraid 3.0</a> assembly method creating the part <a href=http://parts.igem.org/Part:BBa_K2656021> BBa_K2656021</a>. Then, we created the part <a href=http://parts.igem.org/Part:BBa_K2656020> BBa_K2656020</a>. This part is the CDS of the YFP with the addition of the degradation tag ssRA LVA.
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The BioBrick chosen to be improved was the YFP coding sequence: <a href="http://parts.igem.org/Part:BBa_K592101"> BBa_K592101</a>. To do this, we first standardized the part to be compatible with the <a href="https://2018.igem.org/Team:Valencia_UPV/GB3">  GoldenBraid 3.0</a> assembly method creating the part <a href="http://parts.igem.org/Part:BBa_K2656021"> BBa_K2656021</a>. Then, we created the part <a href="http://parts.igem.org/Part:BBa_K2656020"> BBa_K2656020</a>. This part is the CDS of the YFP with the addition of the degradation tag ssRA LVA.
  
 
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To test the effect of the degradation tag, we designed an <a href=https://2018.igem.org/Team:Valencia_UPV/Model#tag_exp>experiment</a> with which we measured the increase in protein degradation due to this tag. To perform this experiment, we assembled two composite parts with the same promoter, RBS and terminator:
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To test the effect of the degradation tag, we designed an <a href="https://2018.igem.org/Team:Valencia_UPV/Model#tag_exp">experiment</a> with which we measured the increase in protein degradation due to this tag. To perform this experiment, we assembled two composite parts with the same promoter, RBS and terminator:
 
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<a href=http://parts.igem.org/Part:BBa_K2656112> BBa_K2656112</a>:
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<a href="http://parts.igem.org/Part:BBa_K2656112"> BBa_K2656112</a>:
 
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<li><p><a href="http://parts.igem.org/Part:BBa_K2656004">BBa_K2656004</a>: the <a href="http://parts.igem.org/Part:BBa_J23106">J23106</a> promoter in its GoldenBraid compatible version from our <a href="https://2018.igem.org/Team:Valencia_UPV/Part_Collection">Part Collection</a></p></li>
 
<li><p><a href="http://parts.igem.org/Part:BBa_K2656004">BBa_K2656004</a>: the <a href="http://parts.igem.org/Part:BBa_J23106">J23106</a> promoter in its GoldenBraid compatible version from our <a href="https://2018.igem.org/Team:Valencia_UPV/Part_Collection">Part Collection</a></p></li>
 
<li><p><a href="http://parts.igem.org/Part:BBa_K2656009">BBa_K2656009</a>: the <a href="http://parts.igem.org/Part:BBa_B0030">B0030</a> ribosome biding site in its GoldenBraid compatible version from our <a href="https://2018.igem.org/Team:Valencia_UPV/Part_Collection">Part Collection</a></p></li>
 
<li><p><a href="http://parts.igem.org/Part:BBa_K2656009">BBa_K2656009</a>: the <a href="http://parts.igem.org/Part:BBa_B0030">B0030</a> ribosome biding site in its GoldenBraid compatible version from our <a href="https://2018.igem.org/Team:Valencia_UPV/Part_Collection">Part Collection</a></p></li>
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<li><p><a href="http://parts.igem.org/Part:BBa_K2656021">BBa_K2656021</a>: The original part  <a href=http://parts.igem.org/Part:BBa_K592101> BBa_K592101</a> compatible with the GoldenBraid assembly method</p></li>
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<li><p><a href="http://parts.igem.org/Part:BBa_K2656021">BBa_K2656021</a>: The original part  <a href="http://parts.igem.org/Part:BBa_K592101"> BBa_K592101</a> compatible with the GoldenBraid assembly method</p></li>
 
<li><p><a href="http://parts.igem.org/Part:BBa_K2656026">BBa_K2656026</a>: the <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a> transcriptional terminator in its GoldenBraid compatible version from our <a href="https://2018.igem.org/Team:Valencia_UPV/Part_Collection">Part Collection</a></p></li>
 
<li><p><a href="http://parts.igem.org/Part:BBa_K2656026">BBa_K2656026</a>: the <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a> transcriptional terminator in its GoldenBraid compatible version from our <a href="https://2018.igem.org/Team:Valencia_UPV/Part_Collection">Part Collection</a></p></li>
 
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<a href=http://parts.igem.org/Part:BBa_K2656111> BBa_K2656111</a>:
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<a href="http://parts.igem.org/Part:BBa_K2656111"> BBa_K2656111</a>:
 
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<li><p><a href="http://parts.igem.org/Part:BBa_K2656026">BBa_K2656026</a></p></li>
 
<li><p><a href="http://parts.igem.org/Part:BBa_K2656026">BBa_K2656026</a></p></li>
 
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Revision as of 13:45, 8 October 2018

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Part Improvement

The BioBrick chosen to be improved was the YFP coding sequence: BBa_K592101. To do this, we first standardized the part to be compatible with the GoldenBraid 3.0 assembly method creating the part BBa_K2656021. Then, we created the part BBa_K2656020. This part is the CDS of the YFP with the addition of the degradation tag ssRA LVA.

To test the effect of the degradation tag, we designed an experiment with which we measured the increase in protein degradation due to this tag. To perform this experiment, we assembled two composite parts with the same promoter, RBS and terminator:

CONTACT US igem.upv.2018@gmail.com