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+ | <img style="width:100%" src="https://static.igem.org/mediawiki/2018/a/a8/T--Cardiff_Wales--PartsBanner.png"> | ||
+ | <div class="maincontent"> | ||
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− | + | <h1>Parts</h1> | |
− | + | <p> Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the <a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a> phytobrick. | |
− | <div class="column | + | <br><br> |
− | < | + | Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>, and all but one are individual parts. The exception is <a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011,</a> which is a part ordered as a composite gBlock and then introduced into <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>. |
− | + | <br><br> | |
− | + | <p>Although they have not been submitted to the registry we have made and tested several <a href="https://2018.igem.org/Team:Cardiff_Wales/Composite_Part">composite level 1 PhytoBricks</a> as well as adding data to existing phytobricks.</p> | |
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+ | <!-- | ||
+ | <groupparts>iGEM18 Cardiff_Wales</groupparts> --> | ||
− | < | + | <br><br> |
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+ | <center> | ||
+ | <br><br> | ||
+ | <table style="width:90%"> | ||
+ | <tr> | ||
+ | <th>Name</th> | ||
+ | <th>Type</th> | ||
+ | <th>Description</th> | ||
+ | <th>Designer</th> | ||
+ | <th>Length</th> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2810000">BBa_K2810000</a></td> | ||
+ | <td>Regulatory</td> | ||
+ | <td>WRKY intron for plant-targeted RNAi constructs </td> | ||
+ | <td>Geraint Parry</td> | ||
+ | <td>295bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2810001">BBa_K2810001</a></td> | ||
+ | <td>Reporter</td> | ||
+ | <td>GUS reporter for plant expression </td> | ||
+ | <td>Geraint Parry</td> | ||
+ | <td>1817bp</td> | ||
+ | </tr> | ||
− | < | + | <tr> |
− | < | + | <td><a href="http://parts.igem.org/Part:BBa_K2810002">BBa_K2810002</a></td> |
− | + | <td>Regulatory</td> | |
− | + | <td>RTBV Vascular Specific Promotor </td> | |
+ | <td>Geraint Parry</td> | ||
+ | <td>759bp</td> | ||
+ | </tr> | ||
− | < | + | <tr> |
− | + | <td><a href="http://parts.igem.org/Part:BBa_K2810003">BBa_K2810003</a></td> | |
− | < | + | <td>RNA</td> |
− | < | + | <td>Full sequence for Myzus persisae BCR3 secreted protein </td> |
− | < | + | <td>Geraint Parry</td> |
− | < | + | <td>257bp</td> |
− | </ | + | </tr> |
− | </ | + | |
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2810004">BBa_K2810004</a></td> | ||
+ | <td>RNA</td> | ||
+ | <td>Complimentary sequence to Myzus persisae BCR3 secreted protein </td> | ||
+ | <td>Geraint Parry</td> | ||
+ | <td>257bp</td> | ||
+ | </tr> | ||
− | < | + | <tr> |
+ | <td><a href="http://parts.igem.org/Part:BBa_K2810005">BBa_K2810005</a></td> | ||
+ | <td>RNA</td> | ||
+ | <td>Coding sequence from 5' region of Myzus persisae BCR3 secreted protein </td> | ||
+ | <td>Geraint Parry</td> | ||
+ | <td>102bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2810006">BBa_K2810006</a></td> | ||
+ | <td>RNA</td> | ||
+ | <td>Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein </td> | ||
+ | <td>Geraint Parry</td> | ||
+ | <td>102bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2810007">BBa_K2810007</a></td> | ||
+ | <td>RNA</td> | ||
+ | <td>siRNA from 5' region of Myzus persicae SP3 secreted protein </td> | ||
+ | <td>Ryan Coates</td> | ||
+ | <td>291bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2810008">BBa_K2810008</a></td> | ||
+ | <td>RNA</td> | ||
+ | <td>siRNA* from 5' region of Myzus persicae SP3 secreted protein </td> | ||
+ | <td>Ryan Coates</td> | ||
+ | <td>291bp</td> | ||
+ | </tr> | ||
− | < | + | <tr> |
− | + | <td><a href="http://parts.igem.org/Part:BBa_K2810009">BBa_K2810009</a></td> | |
− | < | + | <td>Reporter</td> |
− | + | <td>mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible</td> | |
− | + | <td>Ryan Coates</td> | |
− | + | <td>676bp</td> | |
− | + | </tr> | |
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+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2810010">BBa_K2810010</a></td> | ||
+ | <td>Regulatory</td> | ||
+ | <td>Regulatory region derived from the ribitol-responsive operon </td> | ||
+ | <td>Ryan Coates</td> | ||
+ | <td>169bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>mCherry under control of the bacterial promoter and terminato </td> | ||
+ | <td>Ryan Coates</td> | ||
+ | <td>858bp</td> | ||
+ | </tr> | ||
+ | |||
+ | </table> | ||
</html> | </html> |
Latest revision as of 15:33, 16 October 2018
Parts
Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the pGB-A2 phytobrick.
Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor BBa_P10500, and all but one are individual parts. The exception is BBa_K2810011, which is a part ordered as a composite gBlock and then introduced into BBa_P10500.
Although they have not been submitted to the registry we have made and tested several composite level 1 PhytoBricks as well as adding data to existing phytobricks.
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K2810000 | Regulatory | WRKY intron for plant-targeted RNAi constructs | Geraint Parry | 295bp |
BBa_K2810001 | Reporter | GUS reporter for plant expression | Geraint Parry | 1817bp |
BBa_K2810002 | Regulatory | RTBV Vascular Specific Promotor | Geraint Parry | 759bp |
BBa_K2810003 | RNA | Full sequence for Myzus persisae BCR3 secreted protein | Geraint Parry | 257bp |
BBa_K2810004 | RNA | Complimentary sequence to Myzus persisae BCR3 secreted protein | Geraint Parry | 257bp |
BBa_K2810005 | RNA | Coding sequence from 5' region of Myzus persisae BCR3 secreted protein | Geraint Parry | 102bp |
BBa_K2810006 | RNA | Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein | Geraint Parry | 102bp |
BBa_K2810007 | RNA | siRNA from 5' region of Myzus persicae SP3 secreted protein | Ryan Coates | 291bp |
BBa_K2810008 | RNA | siRNA* from 5' region of Myzus persicae SP3 secreted protein | Ryan Coates | 291bp |
BBa_K2810009 | Reporter | mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible | Ryan Coates | 676bp |
BBa_K2810010 | Regulatory | Regulatory region derived from the ribitol-responsive operon | Ryan Coates | 169bp |
BBa_K2810011 | Composite | mCherry under control of the bacterial promoter and terminato | Ryan Coates | 858bp |