Difference between revisions of "Team:Cardiff Wales/Parts"

 
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<h1>Parts</h1>
 
<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p> Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the <a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a> phytobrick.
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<br><br>
 +
Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>, and all but one are individual parts. The exception is <a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011,</a> which is a part ordered as a composite gBlock and then introduced into <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>.
 +
<br><br>
 +
<p>Although they have not been submitted to the registry we have made and tested several <a href="https://2018.igem.org/Team:Cardiff_Wales/Composite_Part">composite level 1 PhytoBricks</a> as well as adding data to existing phytobricks.</p>
 
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<groupparts>iGEM18 Cardiff_Wales</groupparts> -->
  
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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<h3>Inspiration</h3>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h3>Part Table </h3>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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</html>
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<groupparts>iGEM18 Cardiff_Wales</groupparts>
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   <tr>
 
   <tr>
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     <th>Registry link</th>
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     <th>Name</th>
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     <th>Part</th>
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     <th>Type</th>
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     <th>Plasmid</th>
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     <th>Description</th>
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     <th>Summary</th>
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     <th>Designer</th>
 
     <th>Length</th>
 
     <th>Length</th>
 
      
 
      
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
     <td><a href="http://parts.igem.org/Part:BBa_K2404000">BBa_K2404000</a></td>
+
     <td><a href="http://parts.igem.org/Part:BBa_K2810000">BBa_K2810000</a></td>
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     <td>TSH</td>
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     <td>Regulatory</td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a> </td>
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     <td>WRKY intron for plant-targeted RNAi constructs </td>
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     <td>A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does not</b> have His-tags for purification </td>
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     <td>Geraint Parry</td>
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<td>780bp</td>
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<td>295bp</td>
 
   </tr>
 
   </tr>
−
  <tr>
+
 
−
      <td><a href="http://parts.igem.org/Part:BBa_K2404001">BBa_K2404001</a></td>
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<tr>
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     <td>TSHH</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2810001">BBa_K2810001</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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     <td>Reporter</td>
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     <td> A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does</b> have His-tags for purification</td>
+
     <td>GUS reporter for plant expression </td>
−
<td>832bp</td>
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     <td>Geraint Parry</td>
 +
<td>1817bp</td>
 
   </tr>
 
   </tr>
−
  <tr>
+
 
−
      <td><a href="http://parts.igem.org/Part:BBa_K2404002">BBa_K2404002</a></td>
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<tr>
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     <td>PDF1.2</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2810002">BBa_K2810002</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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     <td>Regulatory</td>
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     <td>An inducible promoter that responds to the presence of jasmonic acid in the cell</td>
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     <td>RTBV Vascular Specific Promotor </td>
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<td>522bp</td>
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     <td>Geraint Parry</td>
 +
<td>759bp</td>
 
   </tr>
 
   </tr>
−
  <tr>
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     <td><a href="http://parts.igem.org/Part:BBa_K2404003">BBa_K2404003</a></td>
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<tr>
−
     <td>PR2</td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810003">BBa_K2810003</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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     <td>RNA</td>
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     <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
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     <td>Full sequence for Myzus persisae BCR3 secreted protein </td>
−
<td>519bp</td>
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     <td>Geraint Parry</td>
 +
<td>257bp</td>
 
   </tr>
 
   </tr>
−
  <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_K2404004">BBa_K2404004</a></td>
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<tr>
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     <td>GST6</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2810004">BBa_K2810004</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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     <td>RNA</td>
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     <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
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     <td>Complimentary sequence to Myzus persisae BCR3 secreted protein </td>
−
<td>519bp</td>
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     <td>Geraint Parry</td>
 +
<td>257bp</td>
 
   </tr>
 
   </tr>
 +
 
<tr>
 
<tr>
−
      <td><a href="http://parts.igem.org/Part:BBa_K2404005">BBa_K2404005</a></td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2810005">BBa_K2810005</a></td>
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     <td>WRKY30</td>
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     <td>RNA</td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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     <td>Coding sequence from 5' region of Myzus persisae BCR3 secreted protein </td>
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     <td>An inducible promoter that responds to the presence of cellulose-derived oligomers<br><i>After submission to the registry we discovered that this promotor sequence was incorrect</i></td>
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     <td>Geraint Parry</td>
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<td>507bp</td>
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<td>102bp</td>
 
   </tr>
 
   </tr>
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  <tr>
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      <td><a href="http://parts.igem.org/Part:BBa_K2404006">BBa_K2404006</a></td>
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<tr>
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     <td>Luc+</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2810006">BBa_K2810006</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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     <td>RNA</td>
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     <td>A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression. <i>Prior to submission to the registry we discovered that this coding sequence was incorrect so it was not submitted. In order to generate the level 1 clones containing LUC+ (<a href="http://parts.igem.org/Part:BBa_K2404013">BBa_K2404013</a>, <a href="http://parts.igem.org/Part:BBa_K2404014">BBa_K240414</a>) we used a previously characterised LUC+ protein that is contained within a different (not P10500) level 0 plasmid</i> </td>
+
     <td>Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein </td>
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<td>1653bp</td>
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     <td>Geraint Parry</td>
 +
<td>102bp</td>
 
   </tr>
 
   </tr>
−
  <tr>
+
 
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      <td><a href="http://parts.igem.org/Part:BBa_P10401">BBa_P10401</a></td>
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<tr>
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     <td>NosT</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2810007">BBa_K2810007</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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     <td>RNA</td>
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     <td>A terminator from the nopaline synthase gene which is native to the  <i> Agrobacterium </i> tumor-inducing plasmid pTiT37</td>
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     <td>siRNA from 5' region of Myzus persicae SP3 secreted protein </td>
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<td>285bp</td>
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     <td>Ryan Coates</td>
 +
<td>291bp</td>
 
   </tr>
 
   </tr>
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  <tr>
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        <td><a href="http://parts.igem.org/Part:BBa_P10000">BBa_P10000</a></td>
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<tr>
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     <td>LexA</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2810008">BBa_K2810008</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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     <td>RNA</td>
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     <td>An estradiol inducible promoter from the LexA gene which is native to <i>Escherichia coli </i> </td>
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     <td>siRNA* from 5' region of Myzus persicae SP3 secreted protein </td>
−
<td>362bp</td>
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     <td>Ryan Coates</td>
 +
<td>291bp</td>
 
   </tr>
 
   </tr>
−
  <tr>
+
 
−
      <td><a href="http://parts.igem.org/Part:BBa_P10003">BBa_P10003</a></td>
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<tr>
−
     <td>35S-OTMV</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K2810009">BBa_K2810009</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
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     <td>Reporter</td>
−
     <td>A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus</td>
+
    <td>mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible</td>
−
<td>1446bp</td>
+
    <td>Ryan Coates</td>
 +
<td>676bp</td>
 +
  </tr>
 +
 
 +
<tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2810010">BBa_K2810010</a></td>
 +
    <td>Regulatory</td>
 +
    <td>Regulatory region derived from the ribitol-responsive operon </td>
 +
    <td>Ryan Coates</td>
 +
<td>169bp</td>
 +
  </tr>
 +
<tr>
 +
     <td><a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011</a></td>
 +
     <td>Composite</td>
 +
    <td>mCherry under control of the bacterial promoter and terminato </td>
 +
    <td>Ryan Coates</td>
 +
<td>858bp</td>
 
   </tr>
 
   </tr>
 +
 
 
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Latest revision as of 15:33, 16 October 2018

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Parts

Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the pGB-A2 phytobrick.

Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor BBa_P10500, and all but one are individual parts. The exception is BBa_K2810011, which is a part ordered as a composite gBlock and then introduced into BBa_P10500.

Although they have not been submitted to the registry we have made and tested several composite level 1 PhytoBricks as well as adding data to existing phytobricks.





Name Type Description Designer Length
BBa_K2810000 Regulatory WRKY intron for plant-targeted RNAi constructs Geraint Parry 295bp
BBa_K2810001 Reporter GUS reporter for plant expression Geraint Parry 1817bp
BBa_K2810002 Regulatory RTBV Vascular Specific Promotor Geraint Parry 759bp
BBa_K2810003 RNA Full sequence for Myzus persisae BCR3 secreted protein Geraint Parry 257bp
BBa_K2810004 RNA Complimentary sequence to Myzus persisae BCR3 secreted protein Geraint Parry 257bp
BBa_K2810005 RNA Coding sequence from 5' region of Myzus persisae BCR3 secreted protein Geraint Parry 102bp
BBa_K2810006 RNA Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein Geraint Parry 102bp
BBa_K2810007 RNA siRNA from 5' region of Myzus persicae SP3 secreted protein Ryan Coates 291bp
BBa_K2810008 RNA siRNA* from 5' region of Myzus persicae SP3 secreted protein Ryan Coates 291bp
BBa_K2810009 Reporter mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible Ryan Coates 676bp
BBa_K2810010 Regulatory Regulatory region derived from the ribitol-responsive operon Ryan Coates 169bp
BBa_K2810011 Composite mCherry under control of the bacterial promoter and terminato Ryan Coates 858bp