Difference between revisions of "Team:Cardiff Wales/Parts"

 
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<h1>Parts</h1>
 
<h1>Parts</h1>
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<p> Our team added several useful parts to the PhytoBrick registry, all of which are easy to use with either conventional iGEM cloning or using GoldenGate cloning. While we are happy with the parts we have created, we could only submit basic parts due to the iGEM plasmid backbone specifications, which present problems for submission of level 1 parts made with GoldenGate cloning. For this reason, all of our submitted parts are in the pSB1C3 plasmid, and are all individual parts, with the exception of one part, <a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011,</a>, which is a composite part ordered as a gBlock, so didn't need to be assembled using GoldenGate cloning. We were aware of this limitation starting our project, but thought its ease of use and flexibility outweighed the fact that we wouldn't be able to submit level 1 parts. Nevertheless, we have made and tested several composite parts, adding data to existing registry parts, and to our newer ones.</p>
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<p> Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the <a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a> phytobrick.
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<br><br>
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Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>, and all but one are individual parts. The exception is <a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011,</a> which is a part ordered as a composite gBlock and then introduced into <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>.
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<br><br>
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<p>Although they have not been submitted to the registry we have made and tested several <a href="https://2018.igem.org/Team:Cardiff_Wales/Composite_Part">composite level 1 PhytoBricks</a> as well as adding data to existing phytobricks.</p>
 
</div>
 
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<groupparts>iGEM18 Cardiff_Wales</groupparts> -->
 
<groupparts>iGEM18 Cardiff_Wales</groupparts> -->
  
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<center>
 
<center>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810000">BBa_BBa_K2810000</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810000">BBa_K2810000</a></td>
 
     <td>Regulatory</td>
 
     <td>Regulatory</td>
 
     <td>WRKY intron for plant-targeted RNAi constructs </td>
 
     <td>WRKY intron for plant-targeted RNAi constructs </td>
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<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810001">BBa_BBa_K2810001</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810001">BBa_K2810001</a></td>
 
     <td>Reporter</td>
 
     <td>Reporter</td>
 
     <td>GUS reporter for plant expression </td>
 
     <td>GUS reporter for plant expression </td>
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<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810002">BBa_BBa_K2810002</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810002">BBa_K2810002</a></td>
 
     <td>Regulatory</td>
 
     <td>Regulatory</td>
 
     <td>RTBV Vascular Specific Promotor </td>
 
     <td>RTBV Vascular Specific Promotor </td>
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<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810003">BBa_BBa_K2810003</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810003">BBa_K2810003</a></td>
 
     <td>RNA</td>
 
     <td>RNA</td>
 
     <td>Full sequence for Myzus persisae BCR3 secreted protein </td>
 
     <td>Full sequence for Myzus persisae BCR3 secreted protein </td>
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<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810004">BBa_BBa_K2810004</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810004">BBa_K2810004</a></td>
 
     <td>RNA</td>
 
     <td>RNA</td>
 
     <td>Complimentary sequence to Myzus persisae BCR3 secreted protein </td>
 
     <td>Complimentary sequence to Myzus persisae BCR3 secreted protein </td>
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<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810005">BBa_BBa_K2810005</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810005">BBa_K2810005</a></td>
 
     <td>RNA</td>
 
     <td>RNA</td>
 
     <td>Coding sequence from 5' region of Myzus persisae BCR3 secreted protein </td>
 
     <td>Coding sequence from 5' region of Myzus persisae BCR3 secreted protein </td>
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<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810006">BBa_BBa_K2810006</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810006">BBa_K2810006</a></td>
 
     <td>RNA</td>
 
     <td>RNA</td>
 
     <td>Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein </td>
 
     <td>Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein </td>
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<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810007">BBa_BBa_K2810007</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810007">BBa_K2810007</a></td>
 
     <td>RNA</td>
 
     <td>RNA</td>
 
     <td>siRNA from 5' region of Myzus persicae SP3 secreted protein </td>
 
     <td>siRNA from 5' region of Myzus persicae SP3 secreted protein </td>
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<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810008">BBa_BBa_K2810008</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810008">BBa_K2810008</a></td>
 
     <td>RNA</td>
 
     <td>RNA</td>
 
     <td>siRNA* from 5' region of Myzus persicae SP3 secreted protein </td>
 
     <td>siRNA* from 5' region of Myzus persicae SP3 secreted protein </td>
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<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810009">BBa_BBa_K2810009</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810009">BBa_K2810009</a></td>
 
     <td>Reporter</td>
 
     <td>Reporter</td>
 
     <td>mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible</td>
 
     <td>mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible</td>
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<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810010">BBa_BBa_K2810010</a></td>
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     <td><a href="http://parts.igem.org/Part:BBa_K2810010">BBa_K2810010</a></td>
 
     <td>Regulatory</td>
 
     <td>Regulatory</td>
 
     <td>Regulatory region derived from the ribitol-responsive operon </td>
 
     <td>Regulatory region derived from the ribitol-responsive operon </td>
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   </tr>
 
   </tr>
 
<tr>
 
<tr>
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     <td><a href="http://parts.igem.org/Part:BBa_BBa_K2810011">BBa_BBa_K2810011</a></td>
+
     <td><a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011</a></td>
 
     <td>Composite</td>
 
     <td>Composite</td>
 
     <td>mCherry under control of the bacterial promoter and terminato </td>
 
     <td>mCherry under control of the bacterial promoter and terminato </td>

Latest revision as of 15:33, 16 October 2018

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Parts

Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the pGB-A2 phytobrick.

Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor BBa_P10500, and all but one are individual parts. The exception is BBa_K2810011, which is a part ordered as a composite gBlock and then introduced into BBa_P10500.

Although they have not been submitted to the registry we have made and tested several composite level 1 PhytoBricks as well as adding data to existing phytobricks.





Name Type Description Designer Length
BBa_K2810000 Regulatory WRKY intron for plant-targeted RNAi constructs Geraint Parry 295bp
BBa_K2810001 Reporter GUS reporter for plant expression Geraint Parry 1817bp
BBa_K2810002 Regulatory RTBV Vascular Specific Promotor Geraint Parry 759bp
BBa_K2810003 RNA Full sequence for Myzus persisae BCR3 secreted protein Geraint Parry 257bp
BBa_K2810004 RNA Complimentary sequence to Myzus persisae BCR3 secreted protein Geraint Parry 257bp
BBa_K2810005 RNA Coding sequence from 5' region of Myzus persisae BCR3 secreted protein Geraint Parry 102bp
BBa_K2810006 RNA Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein Geraint Parry 102bp
BBa_K2810007 RNA siRNA from 5' region of Myzus persicae SP3 secreted protein Ryan Coates 291bp
BBa_K2810008 RNA siRNA* from 5' region of Myzus persicae SP3 secreted protein Ryan Coates 291bp
BBa_K2810009 Reporter mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible Ryan Coates 676bp
BBa_K2810010 Regulatory Regulatory region derived from the ribitol-responsive operon Ryan Coates 169bp
BBa_K2810011 Composite mCherry under control of the bacterial promoter and terminato Ryan Coates 858bp