Difference between revisions of "Team:Cardiff Wales/Parts"
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<h1>Parts</h1> | <h1>Parts</h1> | ||
| − | <p> Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite | + | <p> Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the <a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a> phytobrick. |
| − | Therefore each of our submitted parts are in the | + | <br><br> |
| + | Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>, and all but one are individual parts. The exception is <a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011,</a> which is a part ordered as a composite gBlock and then introduced into <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>. | ||
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| + | <p>Although they have not been submitted to the registry we have made and tested several <a href="https://2018.igem.org/Team:Cardiff_Wales/Composite_Part">composite level 1 PhytoBricks</a> as well as adding data to existing phytobricks.</p> | ||
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Latest revision as of 15:33, 16 October 2018
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Parts
Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the pGB-A2 phytobrick.
Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor BBa_P10500, and all but one are individual parts. The exception is BBa_K2810011, which is a part ordered as a composite gBlock and then introduced into BBa_P10500.
Although they have not been submitted to the registry we have made and tested several composite level 1 PhytoBricks as well as adding data to existing phytobricks.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K2810000 | Regulatory | WRKY intron for plant-targeted RNAi constructs | Geraint Parry | 295bp |
| BBa_K2810001 | Reporter | GUS reporter for plant expression | Geraint Parry | 1817bp |
| BBa_K2810002 | Regulatory | RTBV Vascular Specific Promotor | Geraint Parry | 759bp |
| BBa_K2810003 | RNA | Full sequence for Myzus persisae BCR3 secreted protein | Geraint Parry | 257bp |
| BBa_K2810004 | RNA | Complimentary sequence to Myzus persisae BCR3 secreted protein | Geraint Parry | 257bp |
| BBa_K2810005 | RNA | Coding sequence from 5' region of Myzus persisae BCR3 secreted protein | Geraint Parry | 102bp |
| BBa_K2810006 | RNA | Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein | Geraint Parry | 102bp |
| BBa_K2810007 | RNA | siRNA from 5' region of Myzus persicae SP3 secreted protein | Ryan Coates | 291bp |
| BBa_K2810008 | RNA | siRNA* from 5' region of Myzus persicae SP3 secreted protein | Ryan Coates | 291bp |
| BBa_K2810009 | Reporter | mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible | Ryan Coates | 676bp |
| BBa_K2810010 | Regulatory | Regulatory region derived from the ribitol-responsive operon | Ryan Coates | 169bp |
| BBa_K2810011 | Composite | mCherry under control of the bacterial promoter and terminato | Ryan Coates | 858bp |