Difference between revisions of "Team:Cardiff Wales/Parts"
Ryancoates (Talk | contribs) |
|||
| Line 33: | Line 33: | ||
<p> Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the <a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a> phytobrick. | <p> Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the <a href="http://parts.igem.org/Part:BBa_P10503">pGB-A2</a> phytobrick. | ||
<br><br> | <br><br> | ||
| − | Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>, and | + | Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>, and all but one are individual parts. The exception is <a href="http://parts.igem.org/Part:BBa_K2810011">BBa_K2810011,</a> which is a part ordered as a composite gBlock and then introduced into <a href="http://parts.igem.org/Part:BBa_P10500">BBa_P10500</a>. |
<br><br> | <br><br> | ||
<p>Although they have not been submitted to the registry we have made and tested several <a href="https://2018.igem.org/Team:Cardiff_Wales/Composite_Part">composite level 1 PhytoBricks</a> as well as adding data to existing phytobricks.</p> | <p>Although they have not been submitted to the registry we have made and tested several <a href="https://2018.igem.org/Team:Cardiff_Wales/Composite_Part">composite level 1 PhytoBricks</a> as well as adding data to existing phytobricks.</p> | ||
Latest revision as of 15:33, 16 October 2018
With thanks to our sponsors!
Visit our social media pages!
Parts
Our team added several useful parts to the PhytoBrick registry, all of which are compatible for GoldenGate cloning. We are unable to submit level 1 composite phytobricks to the registry as they are held within the pGB-A2 phytobrick.
Therefore each of our submitted parts are in the PhytoBricks Universal Acceptor BBa_P10500, and all but one are individual parts. The exception is BBa_K2810011, which is a part ordered as a composite gBlock and then introduced into BBa_P10500.
Although they have not been submitted to the registry we have made and tested several composite level 1 PhytoBricks as well as adding data to existing phytobricks.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K2810000 | Regulatory | WRKY intron for plant-targeted RNAi constructs | Geraint Parry | 295bp |
| BBa_K2810001 | Reporter | GUS reporter for plant expression | Geraint Parry | 1817bp |
| BBa_K2810002 | Regulatory | RTBV Vascular Specific Promotor | Geraint Parry | 759bp |
| BBa_K2810003 | RNA | Full sequence for Myzus persisae BCR3 secreted protein | Geraint Parry | 257bp |
| BBa_K2810004 | RNA | Complimentary sequence to Myzus persisae BCR3 secreted protein | Geraint Parry | 257bp |
| BBa_K2810005 | RNA | Coding sequence from 5' region of Myzus persisae BCR3 secreted protein | Geraint Parry | 102bp |
| BBa_K2810006 | RNA | Complimentary sequence from 5' region of Myzus persisae BCR3 secreted protein | Geraint Parry | 102bp |
| BBa_K2810007 | RNA | siRNA from 5' region of Myzus persicae SP3 secreted protein | Ryan Coates | 291bp |
| BBa_K2810008 | RNA | siRNA* from 5' region of Myzus persicae SP3 secreted protein | Ryan Coates | 291bp |
| BBa_K2810009 | Reporter | mCherry, codon optimised for Nicotiana benthamiana and GoldenGate compatible | Ryan Coates | 676bp |
| BBa_K2810010 | Regulatory | Regulatory region derived from the ribitol-responsive operon | Ryan Coates | 169bp |
| BBa_K2810011 | Composite | mCherry under control of the bacterial promoter and terminato | Ryan Coates | 858bp |