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             <ul>
 
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                 <li>
 
                 <li>
  
 
                     <ul>
 
                     <ul>
                         <li><a href="#section1">What are we facing?</a></li>
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                         <li><a href="#section1">Overview</a></li>
                         <li><a href="#section2">Predecessors</a></li>
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                         <li><a href="#section2">Tsinghua</a></li>
                         <li><a href="#section3">Project Xscape</a></li>
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                         <li><a href="#section3">SJTU-BioX-Shanghai</a></li>
                         <li><a href="#section4">For Fermentation</a></li>
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                         <li><a href="#section4">GreatBay China</a></li>
                         <li><a href="#section5">For Therapy</a></li>
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                         <li><a href="#section5">BFSUICC-China</a></li>
                         <li><a href="#section6">Metabolic Stress</a></li>
+
                         <li><a href="#section6">Nanjing NFLS</a></li>
                        <li><a href="#section7">DIY Bio and Biosafety</a></li>
+
                        <li><a href="#section8">Community and Future</a></li>
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                        <li><a href="#section9">References</a></li>
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             <div class="topic-title" id="section1">
 
             <div class="topic-title" id="section1">
                 <h3> Overview </h3>
+
                 <h3>Overview</h3>
 
                 <p> RDFZ-China is an active, collaborative team within the iGEM community in China. We share our resources and ideas aiming to create positive impacts on other iGEM teams we collaborated with.  </p>
 
                 <p> RDFZ-China is an active, collaborative team within the iGEM community in China. We share our resources and ideas aiming to create positive impacts on other iGEM teams we collaborated with.  </p>
 
             </div>
 
             </div>
 
             <div class="topic-title" id="section2">
 
             <div class="topic-title" id="section2">
                 <h3>Tsinghua</h3>
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                 <h3><a class="hidden-link" href="https://2018.igem.org/Team:Tsinghua/Collaborations" target="Tsinghua">Tsinghua</a></h3>
                 <p> We provided team Tsinghua with IPTG assay with Ptac in Tsinghua’s IPTG induction devices in their NEON system. Tsinghua was generous to provide us some lab space and equipments to work on our projects. We worked close, both physically and intellectually, to create maximum impacts on our team projects. </p>
+
                 <p> We provided team Tsinghua with IPTG assay with Ptac plasmid backbone in Tsinghua’s IPTG induction devices in their NEON system.Also we provided AHL for their Lux system.Tsinghua was generous to provide us some lab space and equipments to work on our projects. We worked close, both physically and intellectually, to create maximum impacts on our team projects.</p>
 
             </div>
 
             </div>
 
             <div class="topic-title" id="section3">
 
             <div class="topic-title" id="section3">
                 <h3>Project Xscape</h3>
+
                 <h3><a class="hidden-link" href="https://2018.igem.org/Team:SJTU-BioX-Shanghai/Collaborations" target="SJTU-BioX-Shanghai">SJTU-BioX-Shanghai</a></h3>
                <p>Under this circumstance, this year we decided to be a fundamentalist to synthetic biology, by using genetic circuits and logic gates, to establish biosafety devices which can apply to the real-world situation.</p>
+
                 <p> This year, we provided SJTU-BioX-Shanghai with plasmids expressing acoustic reporter genes arg1 and arg2. They were applied to report the location of cancer foci combining with ultrasound in their project “E.Coli for Colon Health Observation”. We also shared protocols and equipments regarding non-invasive bacteria monitoring in our section of gas vesicles.</p>
                 <p>Since cell death and lysis mean there is a continual presence of free DNA in the environment, holins, which are most widely used are excluded from our choices, and colicin E2 nucleases (Darmstadt iGEM2016) came into our site. We choose site non-specific nucleases since the entire genome and plasmids needed to be entirely digested to prevent the spread, and we use nucleases from a different family to prevent the possible evolution of nuclease inhibitors. Artificial DNA, RNA, and amino acids are a good solution, but due to its high cost so far, it is not applicable to most of the user.</p>
+
 
+
 
             </div>
 
             </div>
 
             <div class="topic-title" id="section4">
 
             <div class="topic-title" id="section4">
                 <h3>For fermentation</h3>
+
                 <h3><a class="hidden-link" href="https://2018.igem.org/Team:GreatBay_China/Collaborations" target="Great Bay China">Great Bay China</a></h3>
                 <p>The first device we build is for the fermentation; we want to execute the escaped engineered bacteria from the fermenter, accidentally or intentionally. We used two environment factors to monitor the bacteria’s situation: temperature and population density, they are both high and tunable in the fermenter. So, the device will initiate when temperature and density are both low. We used thermal sensitive regulator (NUS iGEM2017)(Piraner et al.) and quorum sensing regulator (MIT iGEM2004) (Canton et al.)as our sensor, sRNA(Storz et al.) and tetR family repressor PhlF(Glasgow iGEM2015)(Stanton et al.) as the signal inverter. We add intergrase (Peking iGEM2017) controlled by the thermal sensitive regulator, which will turn the promoter of a lethal gene when temperature rise in the fermenter so that bacteria can survive at the very beginning. Also, we build a model to stimulate the minimum autoinducer required at the beginning of the fermentation, same as the purpose of integrase. This model is for keeping bacteria alive at the very beginning of fermentation. Together they form a NOR gate which will lead to cell death through genome degradation when temperature and density decrease.</p>
+
                 <p> Zeyu Tang from the team, RDFZ-China, kindly provided help on modeling for team GBC. To be specific, Zeyu gave them one document about previous work he has done on the fermentor model, which contains deterministic ODEs for predicting the productivity of engineered bacteria in the fermentor. In addition, Zeyu wrote another document which illustrates the deduction of Hill equation, helping them create their model simulation. </p>
 +
                <p> Knowing GreatBay was constructing a constitutive expressing GFP into the bacterial genome, we enquired for it and used it as a capacity monitor for characterizing the expression burden. Greatbay kindly gave it to us as a gift.
 
             </div>
 
             </div>
 
             <div class="topic-title" id="section5">
 
             <div class="topic-title" id="section5">
                 <h3>For Therapy</h3>
+
                 <h3><a href="https://2018.igem.org/Team:BFSUICC-China/Collaborations" class="hidden-link" target="BFSUICC-China">BFSUICC-China</a></h3>
                 <p>The second device we build is for therapeutic bacteria, the device can carry out noninvasive tracing through ultrasound imaging of the gas vesicle(Shapiro et al.), release the drug (from SHSBNU 2017) controlled by a thermal sensitive regulator at nidus by ultrasound tissue heating, and heat to a higher temperature to release nuclease and kill the bacteria after it finishes its mission. </p>
+
                 <p> Our team leader in 2017, Bowen Xiao, did a report on strategies preparing the iGEM competition; then the two teams shared resources and had a conversation on the biggest problems encounter during iGEM. Team BFSUICC-China in September met with us to discuss project updates and Jamboree preparation. </p>
 
             </div>
 
             </div>
            <div class="topic-title" id="section6">
+
          <div class="topic-title" id="section1">
                <h3>For Metabolic Stress</h3>
+
          <h3><a href="https://2018.igem.org/Team:Nanjing_NFLS/Collaboration">Nanjing NFLS</a></h3>
                <p>We applied capacity monitor (Ceroni et al.) to quantify the expression burden of all our systems, and to reduce the metabolic stress, we designed another device for fermentation which used a LuxR repressive promoter (Peking iGEM2011) and cold-regulated 5’UTR region (Ionis Paris 2017). This device only involves one transcriptional regulator, which will be less energy consuming. </p>
+
          <p>At the beginning of their wiki programming, team NFLS faced difficulties with the compatibility of our coding software and the official iGEM server. Experimented from Frontpage to Dreamweaver, they still found several bugs remained in our website. Luckily, we had coded part of their wiki, and helped them with the some of the specific sentences that were only used in iGEM server. As our collaboration proceeded, we shared a lot of coding tips and sentences, including light effects and multiple layers.</p>
            </div>
+
          </div>
            <div class="topic-title" id="section7">
+
                <h3>DIY bio and Biosafey</h3>
+
                <p>Back to the growing and glowing synthetic biology community, despite the ones doing it on campus, more and more people are starting it at home, they call themselves Genehacker or DIY biologists. The lack of sufficient training and efficient surveillance will be a time bomb which we do know there will be a monstrous harmful bioproduct will be made someday in the future, and indeed, it will be a significant threat to the current biosafety basis. Recall our memory to iGEM2009, Peking surveyed DIY bio, almost ten years later, we conducted a similar DIY bio-survey again. We tried to order materials for molecular experiments, using the delivery address to our home, the result was quite shocking that we can buy almost everything for the molecular experiment, from the internet. Then, we went through relevant laws and regulations throughout the world, which we found out that there are no laws related to the credit certification and the address certification about the people who book the biology reagent. Most of the laws are about the quality certification and how they would serve the user after they bought this. We interviewed the Director of the center for disease control and prevention. He said that within his experiment with the disease caused by the Bacteria leak, environmental pollution, the vast impact had been caused. Our country has been making all effort which is the highest effort that we have made in the history. He said it is not easy to solve the problem with hard work, it needs the cooperation between all the countries. He made an example of 731 army during the second world war two, the outbreak of pathogens can cause significant social harm. We are still on our way to win the battle, but the effort still needs to be put in.</p>
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            </div>
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            <div class="topic-title" id="section8">
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                <h3>Community and Future</h3>
+
                <p>Also, we hosted two major meeting in Beijing, a Biosafety Forum in October, we invited team leader who runs his high school lab, lab teacher from a university lab, and a former team member from Peking iGEM2009, who participated in that DIY bio investigation ten years ago.</p>
+
                <p>We concluded that the development of DIY bio should be taken seriously, and the permanent way to solve it is through implanting Biosafety awareness into our academic culture. Also, as iGEMer, we should strive to be the considerable and responsible leaders in our community, to ensure the biosafety issue has been taken properly. Another meeting was with biology Olympians all around China, we discussed the future of biology community during the meeting, especially with more and more high school iGEM teams coming up in China, but lack of relevant instruction and education to the students. We came up with the idea of setting up a collaboration between school to share and overcome difficulties hand in hand. This kind of meeting will be continued after iGEM2018, since the community usually grows fast after every iGEM season. </p>
+
                <p>Hopefully, years later, biosafety awareness and considerations can be seriously taken in communities, laboratory studies, and real-world applications.</p>
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        <div class="reference">
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            <div class="topic-title" id="section9">
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                <h3>References</h3>
+
                <p>Canton, Barry, et al. “Refinement and Standardization of Synthetic Biological Parts and Devices.” Nature Biotechnology, vol. 26, no. 7, 2008, pp. 787–93, doi:10.1038/nbt1413.</p>
+
                <p>Ceroni, Francesca, et al. “Quantifying Cellular Capacity Identifies Gene Expression Designs with Reduced Burden.” Nature Methods, vol. 12, no. 5, 2015, pp. 415–18, doi:10.1038/nmeth.3339.</p>
+
                <p>Lee, Jeong Wook, et al. “Next-Generation Biocontainment Systems for Engineered Organisms.” Nature Chemical Biology, Springer US, 2018, p. 1, doi:10.1038/s41589-018-0056-x.</p>
+
                <p>Moe-Behrens, Gerd H. G., et al. “Preparing Synthetic Biology for the World.” Frontiers in Microbiology, vol. 4, no. JAN, 2013, pp. 1–10, doi:10.3389/fmicb.2013.00005.</p>
+
                <p>Piraner, Dan I., et al. “Tunable Thermal Bioswitches for in Vivo Control of Microbial Therapeutics.” Food, Pharmaceutical and Bioengineering Division 2017 - Core Programming Area at the 2017 AIChE Annual Meeting, vol. 2, no. November, Nature Publishing Group, 2017, pp. 695–702, doi:10.1038/nchembio.2233.</p>
+
                <p>Shapiro, Mikhail G., et al. “Biogenic Gas Nanostructures as Ultrasonic Molecular Reporters.” Nature Nanotechnology, vol. 9, no. 4, Nature Publishing Group, 2014, pp. 311–16, doi:10.1038/nnano.2014.32.</p>
+
                <p>Stanton, Brynne C., et al. “Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates.” Nature Chemical Biology, vol. 10, no. 2, 2014, pp. 99–105, doi:10.1038/nchembio.1411.</p>
+
                <p>Storz, Gisela, et al. “Regulation by Small RNAs in Bacteria: Expanding Frontiers.” Molecular Cell, vol. 43, no. 6, 2011, pp. 880–91, doi:10.1016/j.molcel.2011.08.022.</p>
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                <p>Synthetic, How, and Biology Will. “Regenesis: How Synthetic Biology Will Reinvent Nature and Ourselves.” Choice Reviews Online, 2013, doi:10.5860/CHOICE.50-3835.</p>
+
                <p>Wright, Oliver, et al. “Building-in Biosafety for Synthetic Biology.” Microbiology (United Kingdom), vol. 159, no. PART7, 2013, pp. 1021–35, doi:10.1099/mic.0.066308-0.</p>
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Latest revision as of 13:29, 17 October 2018

pdcomic

Overview

RDFZ-China is an active, collaborative team within the iGEM community in China. We share our resources and ideas aiming to create positive impacts on other iGEM teams we collaborated with.

Tsinghua

We provided team Tsinghua with IPTG assay with Ptac plasmid backbone in Tsinghua’s IPTG induction devices in their NEON system.Also we provided AHL for their Lux system.Tsinghua was generous to provide us some lab space and equipments to work on our projects. We worked close, both physically and intellectually, to create maximum impacts on our team projects.

SJTU-BioX-Shanghai

This year, we provided SJTU-BioX-Shanghai with plasmids expressing acoustic reporter genes arg1 and arg2. They were applied to report the location of cancer foci combining with ultrasound in their project “E.Coli for Colon Health Observation”. We also shared protocols and equipments regarding non-invasive bacteria monitoring in our section of gas vesicles.

Great Bay China

Zeyu Tang from the team, RDFZ-China, kindly provided help on modeling for team GBC. To be specific, Zeyu gave them one document about previous work he has done on the fermentor model, which contains deterministic ODEs for predicting the productivity of engineered bacteria in the fermentor. In addition, Zeyu wrote another document which illustrates the deduction of Hill equation, helping them create their model simulation.

Knowing GreatBay was constructing a constitutive expressing GFP into the bacterial genome, we enquired for it and used it as a capacity monitor for characterizing the expression burden. Greatbay kindly gave it to us as a gift.

BFSUICC-China

Our team leader in 2017, Bowen Xiao, did a report on strategies preparing the iGEM competition; then the two teams shared resources and had a conversation on the biggest problems encounter during iGEM. Team BFSUICC-China in September met with us to discuss project updates and Jamboree preparation.

Nanjing NFLS

At the beginning of their wiki programming, team NFLS faced difficulties with the compatibility of our coding software and the official iGEM server. Experimented from Frontpage to Dreamweaver, they still found several bugs remained in our website. Luckily, we had coded part of their wiki, and helped them with the some of the specific sentences that were only used in iGEM server. As our collaboration proceeded, we shared a lot of coding tips and sentences, including light effects and multiple layers.

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