Difference between revisions of "Team:Valencia UPV/Parts"

 
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As we have explained in [página del porqué usamos GB] we are using the Golden Gate assembly method. But we have a problem: There is not a collection of parts as complete as for BioBricks.</p>
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As we have explained in <a href="https://2018.igem.org/Team:Valencia_UPV/Design" target="_blank"> our design page </a>, we are using the <b>Golden Gate assembly method</b>. But we have a problem: there is not a part collection as complete as the BioBrick collection.</p>
 
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In order to solve this problem, we have made a collection of characterized basic parts (promoters, RBS, CDS and terminators), domesticating them to the Golden Braid 3.0 grammar and creating transcriptional units with them.</p>
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In order to solve this, we have create an extensive <a href="https://2018.igem.org/Team:Valencia_UPV/Part_Collection" target="_blank">Part collection</a> of characterized basic parts (promoters, RBS, CDS and terminator) following the GoldenBraid 3.0 standard, and so also compatible with PhytoBricks standard (rfc 106). We have used these parts to create new transcriptional units, such as the ones that Printeria users will be able to construct.</p>
  
 
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The grammar of the basic parts is the same as in MoClo but we have selected The Golden Braid 3.0 assembly method because it allows to create multigenic constructions with a simple binary system: the transcriptional units are assembled in only 2 different destination plasmids. [link a Golden braid].
 
 
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<h3>Part Table </h3>
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<h3>Parts Table </h3>
  
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<p>We have created these parts: </p>
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<p>During the development of the project, the Valencia UPV iGEM team has achieved to synthesize the following parts.</p>
  
 
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Latest revision as of 00:49, 18 October 2018

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Index

    Introduction

    As we have explained in our design page , we are using the Golden Gate assembly method. But we have a problem: there is not a part collection as complete as the BioBrick collection.

    In order to solve this, we have create an extensive Part collection of characterized basic parts (promoters, RBS, CDS and terminator) following the GoldenBraid 3.0 standard, and so also compatible with PhytoBricks standard (rfc 106). We have used these parts to create new transcriptional units, such as the ones that Printeria users will be able to construct.

    Parts Table

    During the development of the project, the Valencia UPV iGEM team has achieved to synthesize the following parts.

    <groupparts class="col-md-12">iGEM18 Valencia_UPV</groupparts>

    CONTACT US igem.upv.2018@gmail.com