Difference between revisions of "Team:Valencia UPV/Improve"

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"> Figure 1. Experimental results of the fluorescence comparison experiment between the transcriptional unit with BBa_K2656021 and the one with BBa_K2656020. </h6>
 
"> Figure 1. Experimental results of the fluorescence comparison experiment between the transcriptional unit with BBa_K2656021 and the one with BBa_K2656020. </h6>
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<table style="width:100%">
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  <tbody><tr>
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    <th><p>Optimized parameters</p></th>
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    <th><p>Values</p></th>
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  </tr>
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  <tr>
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  <td><p>Translation rate <b>p</b></p></td>
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  <td>
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  <ul>
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  <li><p>
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  For YFP with LVA tag <a href="http://parts.igem.org/Part:BBa_K2656020" target="_blank" style="padding-right: 0">BBa_K2656020</a>: p = 0.4622 min<sup>-1</sup>
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  </p></li>
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  <li><p>
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  For YFP without LVA tag  <a href="http://parts.igem.org/Part:BBa_K2656021" target="_blank" style="padding-right: 0">BBa_K2656021</a>: p = 1.053 min<sup>-1</sup>
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  </p></li>
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  </ul>
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  </td></tr>
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  <tr><td><p>PoI degradation rate <b>d<sub>p</sub></b></p></td>
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  <td><p>
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  </p><ul>
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  <li><p>
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  For YFP with LVA tag <a href="http://parts.igem.org/Part:BBa_K2656020" target="_blank" style="padding-right: 0">BBa_K2656020</a>: d<sub>p</sub> = 0.01492 min<sup>-1</sup>
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  </p></li>
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  <li><p>
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  For YFP without LVA tag  <a href="http://parts.igem.org/Part:BBa_K2656021" target="_blank" style="padding-right: 0">BBa_K2656021</a>: d<sub>p</sub> = 0.0080 min<sup>-1</sup>
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  </p></li>
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  </ul> 
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  <p></p></td>
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  </tr>
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  <tr>
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  <td><p>Dilution rate <b><meta charset="utf-8">μ</b></p></td>
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  <td><p>
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  </p><ul>
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  <li><p>
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  For YFP with LVA tag <a href="http://parts.igem.org/Part:BBa_K2656020" target="_blank" style="padding-right: 0">BBa_K2656020</a>: <meta charset="utf-8">μ = 0.01166 min<sup>-1</sup>
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  </p></li>
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  <li><p>
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  For YFP without LVA tag  <a href="http://parts.igem.org/Part:BBa_K2656021" target="_blank" style="padding-right: 0">BBa_K2656021</a>: <meta charset="utf-8">μ = 0.01307 min<sup>-1</sup>
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  </p></li>
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  </ul>
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  <p></p></td>
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</tr></tbody></table>
 
 
 
         </div>
 
         </div>

Revision as of 10:52, 9 October 2018

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Part Improvement

The BioBrick chosen to be improved was the YFP coding sequence: BBa_K592101. To do this, we first standardized the part to be compatible with the GoldenBraid 3.0 assembly method creating the part BBa_K2656021. Then, we created the part BBa_K2656020. This part is the CDS of the YFP with the addition of the degradation tag ssRA LVA.

To test the effect of the degradation tag, we designed an experiment with which we measured the increase in protein degradation due to this tag. To perform this experiment, we assembled two composite parts with the same promoter, RBS and terminator:

Once the experiment was carried out, the results were plotted and figure 1 was obtained, in which we can observe that the growth of the bacteria with both constructions was very similar, while the fluorescence had a clear variation.

Figure 1. Experimental results of the fluorescence comparison experiment between the transcriptional unit with BBa_K2656021 and the one with BBa_K2656020.

Optimized parameters

Values

Translation rate p

PoI degradation rate dp

Dilution rate μ

CONTACT US igem.upv.2018@gmail.com