Difference between revisions of "Team:Valencia UPV/Integrated Human"

Kano Model

Kano model is used to understand the importance of functions or features to a customer [1]. We have used it to improve Printeria and we want it to be a guide for future iGEM teams, thats why we are going to present our work as a example of use.

Kano model is made up of three stages:

1. Pick up the user's voice

   In this phase you have present your project to an audience and then you ask for criticisms or improvements. We did it in the Mustang Art Gallety (MAG) in Elche (Alicante). At the end of the demostration we gathered all the students and teachers and we asked them for demands, improvements or criticisms.

2. Create your paired questions quiz

   With those demands that we will call necessities you have to make a cuestionaire based on paired cuestions. This questions have the next grammar: ¿How do you feel if (a necessity exists in your device)? and ¿How do you feel if (that necessity did no existed in your device)?. As you can observe, it is the same necessity but expresed positively or negatively. Check aout our questionnaire to see an example:

   CUESTIONNAIRE

3. Clasify your demands

   Once you have all the asnwers, you have to complete a series of tables to conclude if that necessity is really important or not. If it is, you should include it in your design because that will increase the satisfaction of the user. If it is not, do not waste time and money implementing something no one will appreciate!

Study case: Kano model in Printeria

When using Kano model you can join public engagement with integrated human. Taking advantage of the fact that we went to MAG (Mustang Art Gallery) in Elche (Alicante) to present our project in front of students and teachers, we decided to demand feedback. The most important necessities were included in the cuestionaire we described above, and then we send it to the students and teachers who went to fill it. The cuestionnaire and the results are avaliable by clicking in the botton "cuestionnaire".

Once we had the answers (more than 20 to be representative), we filled the different tables Kano model is composed to obtain the results desired. Here you can download the document with the tables and what is most important, the conclusions extracted:

Overall conclusions

• Kano model is an strong tool to include feedback in your project
• Kano model rank needs by customer/user satisfaction
• Kano model prevents wasting money and time implementing unnecesary needs
• Kano model allows you to join public engagement and integrated practices
• Kano model allows you to explain why something was designed or not

Safety Problem	Solution
Sterilization of the PCB	How do we clean the PCB without damaging the metalic and plastic components. Well, we decided to copy what flow cabins do. Based on this we decided to use UV led lights to achieve this objective. Furthermore we have programmed an especial reaction that use little droplets with HCl and ethanol to clean and ensure we have perfectly sterilized the PCB.
Sterilization of the microfluidic tubes	The reaction fluid is going to pass through microfluidic tubes and it will contaminate them, to reverse this situation we will use the same special program wich usses droplets with ethanol and HCl.
Protection of the user	We want the user to see what is happening inside Printeria but also we want them to be protected if something wrong happens inside. Thats why we thought that a metacrilate box that cover the machines would be a nice option. Besides, it is useful to pin up the elements whicht will be part of Printera.
Overheating of the PCB	As the PCB has a cold and a hot zone, it is necessary to evacuate the heat generated. The hot zone can achieve more than 80º degrees and can be a potencial danger. A resisitance is responsible of heating and a peltier plate is responsible of chilling. Peltier effect is based in a semiconductor metal where you apply a potential difference. Then, you create a hot part and a cold one in that semiconductor. We use the cold part to extract the energy and stabilize the temperature via conduction of the PCB's hot zone.
Ventilation	The peltier plate is responsible of chilling the PCB but ¿How do we evacuate all the energy generated? Well, the answer is simple, Printeria just needed ventilation holes and watercooling to do it. Watercooling is based in the higher termic capacity of fluids (water in this case) to absorb energy. Water is pumped through microfluidic tubes to reach the peltier and chill it.
Electroporator	This device is capable to reach hig voltages, so if you require one of these in your design first of all, ensure no one can harm himself/herself. This is the reason why we designed our own electroporator in a dedicated and compacted printed circuit to isolate it from any exposure. It can reach until 1600V, which is not a joke.
Tips	Tips which go in the revolver wheel are disposable to ensure the purity of each reaction.

Expert dialogue

Una muy buena forma de enriquecer el proyecto mediante feed-back es hacerlo consultando a expertos en el tema de estudio como ya se ha hecho anteriormente en iGEM. Su opinión es realmente relevante por la reputación que les precede y eso aporta valor al producto. Por ello nos hemos propuesto contactar con artistas del mundo del Bioarte y científicos reputados para que critiquen nuestro proyecto.

María Peñil & Mehmet Berkmen

María Peñil Cobo & Mehmet Berkmen are coworkers in NEB biolabs Boston. María is a Spanish mixed media artist bron in San Vicente de la Barquera. She studied fine arts and has a master in art education. To do her masterpieces she works with natural media like bacteria.

Dr. Mehmet Berkmen is a Turkish-born international microbiologist. Nowadays he is a Senior sciencist at NEB working on genetically engineering bacteria to produce proteins.

Juntos, se dedican a hacer Bioarte con bacterias crecidas en agar lo cual nos fascinó y anó para contctar con ellos y exponerles nuestro proyecto, Printeria. Los resultados de la entrevist apeuden verse en este documento:

Las conclusiones de la entrevista fueron varias:

• Conclusion 1 + Explicación de como se ha integrado en Printeria

• Conclusion 1 + Explicación de como se ha integrado en Printeria

• Conclusion 1 + Explicación de como se ha integrado en Printeria

Mojica

José María Yturralde

Yturralde has a bachelor's degree and a doctorate in fine arts awarded by the Politechnic University of Valencia (UPV). He has been researcher in MIT among other grat things. Between 1968 and 1973 he developed his work "Figuras imposibles". These figures are really interesting because they show us how our space perception works, they seem coherent at first glance but when you observe them in more detail you see there are inconsistent details.

To prove Printerias power in Bioart we decided to do one of those figures (which you can see in public engagement website) and an interview to get some expert feedback from him. Here you can see the full interview we did:

Las conclusiones de la entrevista fueron varias:

• Conclusion 1 + Explicación de como se ha integrado en Printeria

• Conclusion 1 + Explicación de como se ha integrado en Printeria

• Conclusion 1 + Explicación de como se ha integrado en Printeria

Ryan Fobel

Ryan Fobel is CEO of Sci-Bots located in Toronto (Canada). He is also doctor of philosophy and medical biophysics.

Descripción marc de que ahcen y porqué nos llevó a hacer la entrevista. You can sorload the full intervew here:

Ana & Miriam

Me falta saber que hacen estas mujeres y porqué nos interesó su trabajo tanto

Safety design

The European Research Night is a wide-public event, promoted under the EU Programme Horizon 2020, whose objective is to to encourage the scientific/technical youth vocations and lay public interest to science. It involves research organizations, science museums and public organizations all around Europe.

Activity

As part of this event the City of Arts and Science, one of the biggest scientific and cultural leisure complexes in Europe, organized a complete program of free access activities, including interactive experiments, scientific monologues and researchers talks. We were invited to be part of this program to present Printeria to a wide range of interested people from all ages, who listened our speech with great interest.