Difference between revisions of "Team:Valencia UPV/Part Collection"

Revision as of 23:58, 15 October 2018

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Index

Introduction
Basic Parts
Composite Parts
Plasmids

Part Collection

Introduction

Assembly methods using type IIS restriction enzymes (based on the Golden Gate technology) have a great advantage over those using type II: the reaction is performed in a single step, without the need for gel band purification, therefore increasing the efficiency of the assembly and reducing the time consumed. In addition, the Golden Gate technology allows to assemble more than two pieces and the backbone in a single reaction. These characteristics permit the automation of the Golden Gate assembly method, what suits Printeria perfectly.

Despite these advantages of the Golden Gate technology, finding collections of parts in the same standard optimized for E. coli, and well characterized, is very complicated. This is the reason why we decided to create our collection of basic parts. This collection is based on some of the most used parts in E. coli from the Registry of Standard Biological Parts. To create this collection we used the GoldenBraid 3.0 standard . In addition, to give added value to this collection, we have made an exhaustive characterization of these parts.

In our collection, we have included different types of basic parts so that, combining them, we can create composite parts (transcriptional units) with different behaviours: constitutive, inducible, depending on another construction, to implement a more complex circuit, ...

Basic Parts

All our basic parts are in the plasmid BBa_P10500 and are compatible with BioBricks as they do not contain any illegal sites for RFC10. They have not sites for the type IIS restriction enzymes BsaI and BsmBI. For information on how these parts have been designed, see our design page.

Promoters

With respect to promoters, we include three constitutive promoters of different stregths, a strong promoter for T7 phage DNA polymerase, and a promoter for the sigma 32 subunit of E. coli DNA polymerase. In addition, in our collection there are three promoters regulated by a transcription factor: the inducible pBad, positively regulated by AraC in the presence of L-arabinose, and the inducible and repressible lux promoters, positively and negatively regulated by LuxR in the presence of Acyl-homoserine-lactone, respectively.

Part	Original BioBrick	Description
BBa_K2656004	BBa_J23106	Constitutive promoter
BBa_K2656005	BBa_J23102	Constitutive promoter
BBa_K2656007	BBa_J23101	Constitutive promoter
BBa_K2656000	BBa_I719005	Strong promoter for T7 phage DNA polymerase
BBa_K2656001	BBa_K338001	Promoter for the sigma 32 subunit of E. coli DNA polymerase
BBa_K2656006	BBa_K2442101	pBad minimal promoter: positively regulated by AraC in the presence of L-arabinose
BBa_K2656002	BBa_R0061	Lux repressible promoter: negatively regulated by LuxR in the presence of Acyl-homoserine-lactone.
BBa_K2656003	BBa_R0062	Lux inducible promoter: positively regulated by LuxR in the presence of Acyl-homoserine-lactone.

RBS

Regarding the RBS, we have selected five from the Registry of Standard Biological Parts, with different strengths.

Part	Original BioBrick	Description
BBa_K2656008	BBa_J61100	Very weak RBS
BBa_K2656009	BBa_B0030	Strong RBS
BBa_K2656010	BBa_B0032	Weak RBS
BBa_K2656011	BBa_B0034	Medium RBS
BBa_K2656012	BBa_J61101	Very weak RBS

CDS

For CDS we have chosen different coding sequences of reporter proteins: fluoroproteins (sfGFP, mRFP1, YFP and GFPmut3b) and chromoproteins (amilCP). Another choice we have made is the addition of the LVA degradation tag to the mRFP1, YFP and GFPmut3b sequences. We have also selected the BSMT1 enzyme coding sequence that will allow our bacteria to smell like mint in the presence of salicylic acid. Additionally, we have added to the collection the CDS of the transcription factors AraC and LuxR that control our regulatable promoters, as well as the CDS of LuxI that acts producing the Acyl-Homoserine-Lactone to which LuxR responds. For this last CDS, we have codon optimized its sequence for E. Coli. Finally, we have picked a lysis gene from the enterobacteria phage phiX174 which allows us to create genetic circuits which functionality implies the death of part of the population. All these coding sequences are codon optimized for E. coli.

Part	Original BioBrick	Description
BBa_K2656013	BBa_I746916	Superfolder Green fluorescent Protein (sfGFP) coding sequence
BBa_K2656014	BBa_E1010	monomeric Red fluorescent protein (mRFP1) coding sequence
BBa_K2656021	BBa_K592101	Yellow fluorescent protein (YFP) coding sequence
BBa_K2656022	BBa_E0040	Green fluorescent protein (GFPmut3b) coding sequence
BBa_K2656018	BBa_K592009	amilCP (Blue chromoprotein) coding sequence
BBa_K2656024	BBa_K1399001	mRFP1 with the LVA tag coding sequence
BBa_K2656020	None	YFP with the LVA tag coding sequence
BBa_K2656023	BBa_K1399004	GFPmut3b with the LVA tag coding sequence
BBa_K2656025	BBa_J45004	BSMT1 coding sequence
BBa_K2656017	BBa_K2442103	AraC transcription factor coding sequence
BBa_K2656016	BBa_C0062	LuxR transcription factor coding sequence
BBa_K2656019	BBa_C0161	LuxI (Acyl-Homoserine-Lactone synthase) codon optimized coding sequence
BBa_K2656015	None	Lysis gene from the enterobacteria phage phiX174

Transcriptional terminators

Lastly, as far as terminators are concerned, we have only chosen one, as we do not think that introducing several would have an influence on the functioning of Printeria. The terminator chosen is B0015 because it is the most used in E. Coli.

Part	Original BioBrick	Description
BBa_K2656026	BBa_B0015	Double transcriptional terminator

Composite Parts

In order to make the necessary measurements to characterize the basic parts and demonstrate that they are functional, we have built some composite parts by combining a promoter, an RBS, a CDS and the terminator in a GoldenBraid Alpha 1 vector with the Golden Gate assembly protocol .

Printone

With Printeria, one of our main goals is to promote the Microbial Art among society. Thus, we decided to provide the bioartists with a complete ART DNA toolkit for its use with Printeria. As they use living cells as primary material for their artworks, we wanted to produce an extense palette: Printone.

Printone is composed of a colection of transcriptional units assembled with each of our fluorescent and coloured basic parts. By changing the promoters and RBS strengths, a wide range of different tonalities were obtained. Moreover, the LVA tag allowed us to reduces the brightness and so include degraded tones.

Transcriptional unit	Intensity	CDS
BBa_K2656105		GFPmut3b
BBa_K2656106		GFPmut3b
BBa_K2656007		GFPmut3b
BBa_K2656117		GFPmut3b
BBa_K2656108		GFPmut3b+LVA tag
BBa_K2656101		sfGFP
BBa_K2656100		sfGFP
BBa_K2656102		sfGFP
BBa_K2656103		sfGFP
BBa_K2656104		sfGFP
BBa_K2656109		mRFP1
BBa_K2656118		mRFP1
BBa_K2656119		mRFP
BBa_K2656110		mRFP1+LVA tag
BBa_K2656113		amilCP
BBa_K2656120		amilCP
BBa_K2656112		YFP
BBa_K2656110		YFP+LVA tag

Transcriptional units: complete table

Composite Part	Promoter	RBS	CDS	Terminator	Description
BBa_K2656100	BBa_K2656004	BBa_K2656008	BBa_K2656013	BBa_K2656026	sfGFP transcriptional unit 1
BBa_K2656101	BBa_K2656004	BBa_K2656009	BBa_K2656013	BBa_K2656026	sfGFP transcriptional unit 2
BBa_K2656102	BBa_K2656004	BBa_K2656010	BBa_K2656013	BBa_K2656026	sfGFP transcriptional unit 3
BBa_K2656103	BBa_K2656004	BBa_K2656011	BBa_K2656013	BBa_K2656026	sfGFP transcriptional unit 4
BBa_K2656104	BBa_K2656004	BBa_K2656012	BBa_K2656013	BBa_K2656026	sfGFP transcriptional unit 5
BBa_K2656105	BBa_K2656004	BBa_K2656009	BBa_K2656022	BBa_K2656026	GFP transcriptional unit 1
BBa_K2656106	BBa_K2656005	BBa_K2656009	BBa_K2656022	BBa_K2656026	GFP transcriptional unit 2
BBa_K2656107	BBa_K2656007	BBa_K2656009	BBa_K2656022	BBa_K2656026	GFP transcriptional unit 3
BBa_K2656108	BBa_K2656004	BBa_K2656009	Bba_K2656023	BBa_K2656026	GFP_LVA transcriptional unit
BBa_K2656109	BBa_K2656004	BBa_K2656009	Bba_K2656014	BBa_K2656026	mRFP1 transcriptional unit 1
BBa_K2656110	BBa_K2656004	BBa_K2656009	Bba_K2656024	BBa_K2656026	mRFP1_LVA transcriptional unit 1
BBa_K2656111	BBa_K2656004	BBa_K2656009	BBa_K2656020	BBa_K2656026	YFP_LVA transcriptional unit 1
BBa_K2656112	BBa_K2656004	BBa_K2656009	BBa_K2656021	BBa_K2656026	YFP transcriptional unit 1
BBa_K2656113	BBa_K2656004	BBa_K2656009	BBa_K2656018	BBa_K2656026	amilCP transcriptional unit 1
BBa_K2656114	BBa_K2656004	BBa_K2656009	BBa_K2656016	BBa_K2656026	Constitutive LuxR transcriptional unit
BBa_K2656115	BBa_K2656001	BBa_K2656009	BBa_K2656022	BBa_K2656026	HSP-GFPmut3b transcriptional unit
BBa_K2656116	BBa_K2656002	BBa_K2656009	BBa_K2656022	BBa_K2656026	pLuxR-GFPmut3b transcriptional unit
BBa_K2656117	BBa_K2656004	BBa_K2656011	BBa_K2656022	BBa_K2656026	GFPmut3b transcriptional unit 4
BBa_K2656118	BBa_K2656004	BBa_K2656010	BBa_K2656014	BBa_K2656026	mRFP transcriptional unit 2
BBa_K2656119	BBa_K2656004	BBa_K2656011	BBa_K2656014	BBa_K2656026	mRFP transcriptional unit 3
BBa_K2656120	BBa_K2656004	BBa_K2656011	BBa_K2656018	BBa_K2656026	amilCP transcriptional unit 2
BBa_K2656121	BBa_K2656004	BBa_K2656011	BBa_K2656017	BBa_K2656026	AraC constitutive transcriptional unit
BBa_K2656122	BBa_K2656003	BBa_K2656011	BBa_K2656022	BBa_K2656026	pLux-GFPmut3b transcriptional unit
BBa_K2656123	BBa_K2656000	BBa_K2656010	BBa_K2656022	BBa_K2656026	pT7-GFPmut3b transcriptional unit
BBa_K2656124	BBa_K2656000	BBa_K2656011	BBa_K2656022	BBa_K2656026	pT7-GFPmut3b transcriptional unit 2
BBa_K2656125	BBa_K2656004	BBa_K2656009	BBa_K2656019	BBa_K2656026	LuxI constitutive transcriptional unit 1
BBa_K2656126	BBa_K2656004	BBa_K2656009	BBa_K2656025	BBa_K2656026	BMSTI transcriptional unit

Plasmids

Our transcriptional units were built using a GoldenBraid 3.0 alpha 1 vector which is not compatible with the BioBrick grammar. Therefore, we have created the BBa_K2656200 plasmid, adapted from the standard pSB1C3 backbone, to convert our GoldenBraid transcriptional units to BioBricks.

BBa_K2656200 is a modified version of BBa_P10500, so it includes BsmBI restriction sites flanking a RFP selection marker. When digesting this plasmid with BsmBI the result is complementary sticky ends to those formed in a GoldenBraid alpha 1 plasmid when digesting with the same enzyme.

By this way, the BioBrick compatibility for each composite part in our collection is easily achieved using this plasmid as the destination vector in a Golden Gate one-pot reaction with BsmBI.

Part	Description
BBa_K2656200	BioBricks compatible plasmid

@@ Line 476: / Line 476: @@
      margin-bottom: 0;
      color: #353535;">In order to make the necessary measurements to characterize the basic parts and demonstrate that they are functional, we have built some composite parts by combining a promoter, an RBS, a CDS and the terminator in a <a href="https://2018.igem.org/Team:Valencia_UPV/Design#Level1" target="_blank">GoldenBraid Alpha 1 vector</a> <a href="https://static.igem.org/mediawiki/2018/b/bc/T--Valencia_UPV--protocols.pdf#page=27" target="_blank">with the Golden Gate assembly protocol</a> .</p>
+    <h5>Printone</h5>
+    With Printeria, one of our main goals is to promote the Microbial Art among society. Thus, we decided to provide the bioartists with a complete <b>ART DNA toolkit</b> for its use with Printeria. As they use living cells as primary material for their artworks, we wanted to produce an extense palette: Printone.
+    <p> Printone is composed of a colection of transcriptional units assembled with each of our fluorescent and coloured basic parts. By changing the promoters and RBS strengths, a wide range of different tonalities were obtained. Moreover, the LVA tag allowed us to reduces the brightness and so include degraded tones. </p>
+    <div>
+                                <table class="border--round table--alternate-row tableHec" style="width:100%">
+                                    <thead><tr>
+                                    <th class="thHec">Transcriptional unit</th>
+                                    <th class="thHec">Intensity</th>
+                                    <th class="thHec">CDS</th>
+                                    </tr>
+                                    </thead><tbody><tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656004"> BBa_K2656105 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_J23106">   </a> </td>
+                                    <td class="tdHec">
+GFPmut3b
+</td>
+                                    </tr>
+                                    <tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656005"> BBa_K2656106</a></td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_J23102">  </a> </td>
+<td class="tdHec">GFPmut3b</td>
+                                    </tr>
+<tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656007"> BBa_K2656007</a>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_J23101">  </a> </td>
+</td>
+                                    <td class="tdHec">GFPmut3b</td>
+                                    </tr>
+<tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656117"> BBa_K2656117</a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_I719005"> </a> </td>
+                                    <td class="tdHec">GFPmut3b</td>
+                                    </tr>
+<tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656001">BBa_K2656108</a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K338001"></a> </td>
+                                    <td class="tdHec">GFPmut3b+LVA tag</td>
+                                    </tr>
+<tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656006">BBa_K2656101</a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2442101"> </a> </td>
+                                    <td class="tdHec">sfGFP</td>
+                                    </tr>
+<tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656002">BBa_K2656100 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0061"> </a> </td>
+                                    <td class="tdHec"> sfGFP
+</td>
+                                    </tr>
+<tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656102 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">sfGFP
+</td>
+  </tr>
+  <tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656103 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">sfGFP
+</td> </tr>
+  <tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656104 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">sfGFP
+</td> </tr>
+  <tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656109 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">mRFP1
+</td> </tr>
+  <tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656118</a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">mRFP1
+</td> </tr>
+  <tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656119 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">mRFP
+</td> </tr>
+  <tr>
+                                    <td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656110 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">mRFP1+LVA tag
+</td> </tr>
+  <tr>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656113 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">amilCP
+</td> </tr>
+  <tr>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656120 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">amilCP
+</td> </tr>
+  <tr>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656112 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">YFP
+</td> </tr>
+  <tr>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_K2656003"> BBa_K2656110 </a> </td>
+<td class="tdHec"><a href="http://parts.igem.org/Part:BBa_R0062"> </a> </td>
+                                    <td class="tdHec">YFP+LVA tag
+</td>
+                                    </tr>
+                                    </tbody></table>
+                            </div>
+    <p><b>Transcriptional units: complete table</b></p>
 <div>
 <div> <table class="border--round table--alternate-row tableHec" > <thead><tr><th class="thHec"> Composite Part </th> <th class="thHec">Promoter</th><th class="thHec">RBS</th><th class="thHec">CDS</th><th class="thHec">Terminator</th><th class="thHec">Description</th> </tr> </thead><tbody><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656100"target="_blank" >BBa_K2656100</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656008"target="_blank" >BBa_K2656008</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656013"target="_blank" >BBa_K2656013</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">sfGFP transcriptional unit 1</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656101"target="_blank" >BBa_K2656101</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656013"target="_blank" >BBa_K2656013</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">sfGFP transcriptional unit 2</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656102"target="_blank" >BBa_K2656102</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656010"target="_blank" >BBa_K2656010</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656013"target="_blank" >BBa_K2656013</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">sfGFP transcriptional unit 3</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656103"target="_blank" >BBa_K2656103</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656011"target="_blank" >BBa_K2656011</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656013"target="_blank" >BBa_K2656013</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">sfGFP transcriptional unit 4</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656104"target="_blank" >BBa_K2656104</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656012"target="_blank" >BBa_K2656012</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656013"target="_blank" >BBa_K2656013</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">sfGFP transcriptional unit 5</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656105"target="_blank" >BBa_K2656105</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656022"target="_blank" >BBa_K2656022</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">GFP transcriptional unit 1</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656106"target="_blank" >BBa_K2656106</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656005"target="_blank" >BBa_K2656005</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656022"target="_blank" >BBa_K2656022</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">GFP transcriptional unit 2</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656107"target="_blank" >BBa_K2656107</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656007"target="_blank" >BBa_K2656007</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656022"target="_blank" >BBa_K2656022</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">GFP transcriptional unit 3</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656108"target="_blank" >BBa_K2656108</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:Bba_K2656023"target="_blank" >Bba_K2656023</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">GFP_LVA transcriptional unit</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656109"target="_blank" >BBa_K2656109</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:Bba_K2656014"target="_blank" >Bba_K2656014</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">mRFP1 transcriptional unit 1</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656110"target="_blank" >BBa_K2656110</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:Bba_K2656024"target="_blank" >Bba_K2656024</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">mRFP1_LVA transcriptional unit 1</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656111"target="_blank" >BBa_K2656111</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656020"target="_blank" >BBa_K2656020</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">YFP_LVA transcriptional unit 1</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656112"target="_blank" >BBa_K2656112</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656021"target="_blank" >BBa_K2656021</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">YFP transcriptional unit 1</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656113"target="_blank" >BBa_K2656113</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656018"target="_blank" >BBa_K2656018</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">amilCP transcriptional unit 1</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656114"target="_blank" >BBa_K2656114</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656016"target="_blank" >BBa_K2656016</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">Constitutive LuxR transcriptional unit</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656115"target="_blank" >BBa_K2656115</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656001"target="_blank" >BBa_K2656001</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656022"target="_blank" >BBa_K2656022</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">HSP-GFPmut3b transcriptional unit</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656116"target="_blank" >BBa_K2656116</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656002"target="_blank" >BBa_K2656002</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656022"target="_blank" >BBa_K2656022</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">pLuxR-GFPmut3b transcriptional unit</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656117"target="_blank" >BBa_K2656117</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656011"target="_blank" >BBa_K2656011</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656022"target="_blank" >BBa_K2656022</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">GFPmut3b transcriptional unit 4</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656118"target="_blank" >BBa_K2656118</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656010"target="_blank" >BBa_K2656010</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656014"target="_blank" >BBa_K2656014</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">mRFP transcriptional unit 2</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656119"target="_blank" >BBa_K2656119</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656011"target="_blank" >BBa_K2656011</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656014"target="_blank" >BBa_K2656014</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">mRFP transcriptional unit 3</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656120"target="_blank" >BBa_K2656120</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656011"target="_blank" >BBa_K2656011</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656018"target="_blank" >BBa_K2656018</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">amilCP transcriptional unit 2</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656121"target="_blank" >BBa_K2656121</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656011"target="_blank" >BBa_K2656011</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656017"target="_blank" >BBa_K2656017</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">AraC constitutive transcriptional unit</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656122"target="_blank" >BBa_K2656122</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656003"target="_blank" >BBa_K2656003</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656011"target="_blank" >BBa_K2656011</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656022"target="_blank" >BBa_K2656022</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">pLux-GFPmut3b transcriptional unit</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656123"target="_blank" >BBa_K2656123</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656000"target="_blank" >BBa_K2656000</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656010"target="_blank" >BBa_K2656010</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656022"target="_blank" >BBa_K2656022</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">pT7-GFPmut3b transcriptional unit</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656124"target="_blank" >BBa_K2656124</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656000"target="_blank" >BBa_K2656000</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656011"target="_blank" >BBa_K2656011</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656022"target="_blank" >BBa_K2656022</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">pT7-GFPmut3b transcriptional unit 2</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656125"target="_blank" >BBa_K2656125</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656019"target="_blank" >BBa_K2656019</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">LuxI constitutive transcriptional unit 1</td></tr><tr><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656126"target="_blank" >BBa_K2656126</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656004"target="_blank" >BBa_K2656004</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656009"target="_blank" >BBa_K2656009</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656025"target="_blank" >BBa_K2656025</a></td><td class="tdAlb"><a href="http://parts.igem.org/Part:BBa_K2656026"target="_blank" >BBa_K2656026</a></td><td class="tdAlb">BMSTI transcriptional unit</td></tr></tbody></table></div>
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 <h4 style="
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