Difference between revisions of "Team:Cardiff Wales/Parts"

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  <tr>
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    <th>Registry link</th>
 +
    <th>Part</th>
 +
    <th>Plasmid</th>
 +
    <th>Summary</th>
 +
    <th>Length</th>
 +
   
 +
  </tr>
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  <tr>
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    <td><a href="http://parts.igem.org/Part:BBa_K2404000">BBa_K2404000</a></td>
 +
    <td>TSH</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a> </td>
 +
    <td>A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does not</b> have His-tags for purification </td>
 +
<td>780bp</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2404001">BBa_K2404001</a></td>
 +
    <td>TSHH</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
 +
    <td> A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant <b>does</b> have His-tags for purification</td>
 +
<td>832bp</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2404002">BBa_K2404002</a></td>
 +
    <td>PDF1.2</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
 +
    <td>An inducible promoter that responds to the presence of jasmonic acid in the cell</td>
 +
<td>522bp</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2404003">BBa_K2404003</a></td>
 +
    <td>PR2</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
 +
    <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
 +
<td>519bp</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2404004">BBa_K2404004</a></td>
 +
    <td>GST6</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
 +
    <td>An inducible promoter that responds to the presence of salicylic acid in the cell</td>
 +
<td>519bp</td>
 +
  </tr>
 +
<tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2404005">BBa_K2404005</a></td>
 +
    <td>WRKY30</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
 +
    <td>An inducible promoter that responds to the presence of cellulose-derived oligomers<br><i>After submission to the registry we discovered that this promotor sequence was incorrect</i></td>
 +
<td>507bp</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2404006">BBa_K2404006</a></td>
 +
    <td>Luc+</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
 +
    <td>A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression. <i>Prior to submission to the registry we discovered that this coding sequence was incorrect so it was not submitted. In order to generate the level 1 clones containing LUC+ (<a href="http://parts.igem.org/Part:BBa_K2404013">BBa_K2404013</a>, <a href="http://parts.igem.org/Part:BBa_K2404014">BBa_K240414</a>) we used a previously characterised LUC+ protein that is contained within a different (not P10500) level 0 plasmid</i> </td>
 +
<td>1653bp</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_P10401">BBa_P10401</a></td>
 +
    <td>NosT</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
 +
    <td>A terminator from the nopaline synthase gene which is native to the  <i> Agrobacterium </i> tumor-inducing plasmid pTiT37</td>
 +
<td>285bp</td>
 +
  </tr>
 +
  <tr>
 +
        <td><a href="http://parts.igem.org/Part:BBa_P10000">BBa_P10000</a></td>
 +
    <td>LexA</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
 +
    <td>An estradiol inducible promoter from the LexA gene which is native to <i>Escherichia coli </i> </td>
 +
<td>362bp</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_P10003">BBa_P10003</a></td>
 +
    <td>35S-OTMV</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_P10500">P10500</a></td>
 +
    <td>A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus</td>
 +
<td>1446bp</td>
 +
  </tr>
 +
</table>
  
 
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Revision as of 10:08, 26 September 2018

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Parts

Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <groupparts> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.

Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.

Note

Note that parts must be documented on the Registry. This page serves to showcase the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.

Adding parts to the registry

You can add parts to the Registry at our Add a Part to the Registry link.

We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you do need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)

ADD PARTS

Inspiration

We have a created a collection of well documented parts that can help you get started.

You can also take a look at how other teams have documented their parts in their wiki:

What information do I need to start putting my parts on the Registry?

The information needed to initially create a part on the Registry is:

  • Part Name
  • Part type
  • Creator
  • Sequence
  • Short Description (60 characters on what the DNA does)
  • Long Description (Longer description of what the DNA does)
  • Design considerations

We encourage you to put up much more information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page.

Part Table

Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.

<groupparts>iGEM18 Cardiff_Wales</groupparts>








Registry link Part Plasmid Summary Length
BBa_K2404000 TSH P10500 A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does not have His-tags for purification 780bp
BBa_K2404001 TSHH P10500 A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does have His-tags for purification 832bp
BBa_K2404002 PDF1.2 P10500 An inducible promoter that responds to the presence of jasmonic acid in the cell 522bp
BBa_K2404003 PR2 P10500 An inducible promoter that responds to the presence of salicylic acid in the cell 519bp
BBa_K2404004 GST6 P10500 An inducible promoter that responds to the presence of salicylic acid in the cell 519bp
BBa_K2404005 WRKY30 P10500 An inducible promoter that responds to the presence of cellulose-derived oligomers
After submission to the registry we discovered that this promotor sequence was incorrect		 507bp
BBa_K2404006 Luc+ P10500 A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression. Prior to submission to the registry we discovered that this coding sequence was incorrect so it was not submitted. In order to generate the level 1 clones containing LUC+ (BBa_K2404013, BBa_K240414) we used a previously characterised LUC+ protein that is contained within a different (not P10500) level 0 plasmid 1653bp
BBa_P10401 NosT P10500 A terminator from the nopaline synthase gene which is native to the Agrobacterium tumor-inducing plasmid pTiT37 285bp
BBa_P10000 LexA P10500 An estradiol inducible promoter from the LexA gene which is native to Escherichia coli 362bp
BBa_P10003 35S-OTMV P10500 A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus 1446bp