Difference between revisions of "Team:Valencia UPV/Part Collection"

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     color: #353535;">In order to make the necessary measurements to characterize the basic parts and demonstrate that they are functional, we have built some composite parts by combining a promoter, an RBS, a CDS and the terminator in a GoldenBraid Alpha 1 vector (link a GoldenBraid 3.0) with the Golden Gate assembly protocol (link a protocolos).</p>
 
     color: #353535;">In order to make the necessary measurements to characterize the basic parts and demonstrate that they are functional, we have built some composite parts by combining a promoter, an RBS, a CDS and the terminator in a GoldenBraid Alpha 1 vector (link a GoldenBraid 3.0) with the Golden Gate assembly protocol (link a protocolos).</p>
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<div> <table class="border--round table--alternate-row tableHec" style="width:80%"> <thead><tr><th class="thHec"> Composite Part </th> <th class="thHec">Promoter</th><th class="thHec">RBS</th><th class="thHec">CDS</th><th class="thHec">Terminator</th><th class="thHec">Description</th> </tr> </thead><tbody><tr><td><a href=parts.igem.org/Part:BBa_K2656100>BBa_K2656100</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656008>BBa_K2656008</a></td><td><a href=parts.igem.org/Part:BBa_K2656013>BBa_K2656013</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>sfGFP transcriptional unit 1</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656101>BBa_K2656101</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656013>BBa_K2656013</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>sfGFP transcriptional unit 2</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656102>BBa_K2656102</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656010>BBa_K2656010</a></td><td><a href=parts.igem.org/Part:BBa_K2656013>BBa_K2656013</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>sfGFP transcriptional unit 3</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656103>BBa_K2656103</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656011>BBa_K2656011</a></td><td><a href=parts.igem.org/Part:BBa_K2656013>BBa_K2656013</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>sfGFP transcriptional unit 4</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656104>BBa_K2656104</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656012>BBa_K2656012</a></td><td><a href=parts.igem.org/Part:BBa_K2656013>BBa_K2656013</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>sfGFP transcriptional unit 5</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656105>BBa_K2656105</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>GFP transcriptional unit 1</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656106>BBa_K2656106</a></td><td><a href=parts.igem.org/Part:BBa_K2656005>BBa_K2656005</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>GFP transcriptional unit 2</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656107>BBa_K2656107</a></td><td><a href=parts.igem.org/Part:BBa_K2656007>BBa_K2656007</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>GFP transcriptional unit 3</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656117>BBa_K2656117</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656011>BBa_K2656011</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>GFP transcriptional unit 4</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656108>BBa_K2656108</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:Bba_K2656023>Bba_K2656023</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>GFP_LVA transcriptional unit</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656109>BBa_K2656109</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:Bba_K2656014>Bba_K2656014</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>mRFP1 transcriptional unit 1</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656118>BBa_K2656118</a></td><td><a href=parts.igem.org/Part:BBa_K2656001>BBa_K2656001</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>mRFP1 transcriptional unit 2</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656119>BBa_K2656119</a></td><td><a href=parts.igem.org/Part:BBa_K2656002>BBa_K2656002</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>mRFP1 transcriptional unit 3</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656110>BBa_K2656110</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:Bba_K2656024>Bba_K2656024</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>mRFP1_LVA transcriptional unit 1</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656111>BBa_K2656111</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656021>BBa_K2656021</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>YFP_LVA transcriptional unit 1</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656112>BBa_K2656112</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656018>BBa_K2656018</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>YFP transcriptional unit 1</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656113>BBa_K2656113</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656016>BBa_K2656016</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>amilCP transcriptional unit 1</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656120>BBa_K2656120</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656011>BBa_K2656011</a></td><td><a href=parts.igem.org/Part:BBa_K2656018>BBa_K2656018</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>amilCP transcriptional unit 2</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656115>BBa_K2656115</a></td><td><a href=parts.igem.org/Part:BBa_K2656001>BBa_K2656001</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>HSP-GFPmut3b transcriptional unit</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656114>BBa_K2656114</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656016>BBa_K2656016</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>Constitutive LuxR transcriptional unit</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656116>BBa_K2656116</a></td><td><a href=parts.igem.org/Part:BBa_K2656002>BBa_K2656002</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>pLuxR-GFPmut3b transcriptional unit</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656122>BBa_K2656122</a></td><td><a href=parts.igem.org/Part:BBa_K2656003>BBa_K2656003</a></td><td><a href=parts.igem.org/Part:BBa_K2656011>BBa_K2656011</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>pLux-GFPmut3b transcriptional unit</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656125>BBa_K2656125</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656019>BBa_K2656019</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>Constitutive LuxI transcriptional unit 1</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656123>BBa_K2656123</a></td><td><a href=parts.igem.org/Part:BBa_K2656000>BBa_K2656000</a></td><td><a href=parts.igem.org/Part:BBa_K2656010>BBa_K2656010</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>pT7-GFPmut3b transcriptional unit 1</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656124>BBa_K2656124</a></td><td><a href=parts.igem.org/Part:BBa_K2656000>BBa_K2656000</a></td><td><a href=parts.igem.org/Part:BBa_K2656011>BBa_K2656011</a></td><td><a href=parts.igem.org/Part:BBa_K2656022>BBa_K2656022</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>pT7-GFPmut3b transcriptional unit 2</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656121>BBa_K2656121</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656011>BBa_K2656011</a></td><td><a href=parts.igem.org/Part:BBa_K2656017>BBa_K2656017</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>AraC constitutive transcriptional unit</td></tr><tr><td><a href=parts.igem.org/Part:BBa_K2656126>BBa_K2656126</a></td><td><a href=parts.igem.org/Part:BBa_K2656004>BBa_K2656004</a></td><td><a href=parts.igem.org/Part:BBa_K2656009>BBa_K2656009</a></td><td><a href=parts.igem.org/Part:BBa_K2656025>BBa_K2656025</a></td><td><a href=parts.igem.org/Part:BBa_K2656026>BBa_K2656026</a></td><td>BMSTI transcriptional unit</td></tr></tbody></table></div>
+
<div> <table class="border--round table--alternate-row tableHec" > <thead><tr><th class="thHec"> Composite Part </th> <th class="thHec">Promoter</th><th class="thHec">RBS</th><th class="thHec">CDS</th><th class="thHec">Terminator</th><th class="thHec">Description</th> </tr> </thead><tbody><tr><td><a href="http://parts.igem.org/Part:BBa_K2656100"">BBa_K2656100</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656008"">BBa_K2656008</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656013"">BBa_K2656013</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>sfGFP transcriptional unit 1</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656101"">BBa_K2656101</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656013"">BBa_K2656013</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>sfGFP transcriptional unit 2</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656102"">BBa_K2656102</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656010"">BBa_K2656010</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656013"">BBa_K2656013</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>sfGFP transcriptional unit 3</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656103"">BBa_K2656103</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656011"">BBa_K2656011</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656013"">BBa_K2656013</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>sfGFP transcriptional unit 4</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656104"">BBa_K2656104</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656012"">BBa_K2656012</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656013"">BBa_K2656013</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>sfGFP transcriptional unit 5</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656105"">BBa_K2656105</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>GFP transcriptional unit 1</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656106"">BBa_K2656106</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656005"">BBa_K2656005</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>GFP transcriptional unit 2</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656107"">BBa_K2656107</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656007"">BBa_K2656007</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>GFP transcriptional unit 3</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656117"">BBa_K2656117</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656011"">BBa_K2656011</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>GFP transcriptional unit 4</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656108"">BBa_K2656108</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:Bba_K2656023"">Bba_K2656023</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>GFP_LVA transcriptional unit</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656109"">BBa_K2656109</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:Bba_K2656014"">Bba_K2656014</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>mRFP1 transcriptional unit 1</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656118"">BBa_K2656118</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656001"">BBa_K2656001</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>mRFP1 transcriptional unit 2</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656119"">BBa_K2656119</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656002"">BBa_K2656002</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>mRFP1 transcriptional unit 3</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656110"">BBa_K2656110</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:Bba_K2656024"">Bba_K2656024</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>mRFP1_LVA transcriptional unit 1</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656111"">BBa_K2656111</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656021"">BBa_K2656021</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>YFP_LVA transcriptional unit 1</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656112"">BBa_K2656112</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656018"">BBa_K2656018</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>YFP transcriptional unit 1</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656113"">BBa_K2656113</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656016"">BBa_K2656016</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>amilCP transcriptional unit 1</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656120"">BBa_K2656120</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656011"">BBa_K2656011</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656018"">BBa_K2656018</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>amilCP transcriptional unit 2</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656115"">BBa_K2656115</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656001"">BBa_K2656001</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>HSP-GFPmut3b transcriptional unit</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656114"">BBa_K2656114</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656016"">BBa_K2656016</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>Constitutive LuxR transcriptional unit</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656116"">BBa_K2656116</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656002"">BBa_K2656002</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>pLuxR-GFPmut3b transcriptional unit</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656122"">BBa_K2656122</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656003"">BBa_K2656003</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656011"">BBa_K2656011</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>pLux-GFPmut3b transcriptional unit</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656125"">BBa_K2656125</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656019"">BBa_K2656019</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>Constitutive LuxI transcriptional unit 1</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656123"">BBa_K2656123</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656000"">BBa_K2656000</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656010"">BBa_K2656010</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>pT7-GFPmut3b transcriptional unit 1</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656124"">BBa_K2656124</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656000"">BBa_K2656000</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656011"">BBa_K2656011</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656022"">BBa_K2656022</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>pT7-GFPmut3b transcriptional unit 2</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656121"">BBa_K2656121</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656011"">BBa_K2656011</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656017"">BBa_K2656017</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>AraC constitutive transcriptional unit</td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2656126"">BBa_K2656126</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656004"">BBa_K2656004</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656009"">BBa_K2656009</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656025"">BBa_K2656025</a></td><td><a href="http://parts.igem.org/Part:BBa_K2656026"">BBa_K2656026</a></td><td>BMSTI transcriptional unit</td></tr></tbody></table></div>
 
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Revision as of 09:12, 11 October 2018

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Index

    Part Collection

    Introduction

    Assembly methods using type IIS restriction enzymes (based on the Golden Gate technology) have a great advantage over those using type II: the reaction is performed in a single step, without the need for gel band purification, therefore increasing the efficiency of the assembly and reducing the time consumed. In addition, the Golden Gate technology allows to assemble more than two pieces and the backbone in a single reaction. These characteristics permit the automation of the Golden Gate assembly method, what suits Printeria perfectly.

    Despite these advantages of the Golden Gate technology, finding collections of parts in the same standard optimized for E. coli, and well characterized, is very complicated. This is the reason why we decided to create our collection of basic parts. This collection is based on some of the most used parts in E. coli from the Registry of Standard Biological Parts. To create this collection we used the GoldenBraid 3.0 standard . In addition, to give added value to this collection, we have made an exhaustive characterization of these parts.

    In our collection, we have included different types of basic parts so that, combining them, we can create composite parts (transcriptional units) with different behaviours: constitutive, inducible, depending on another construction, to implement a more complex circuit, ...

    Basic Parts

    All our basic parts are in the plasmid BBa_P10500 and are compatible with BioBricks as they do not contain any illegal sites for RFC10. They have not sites for the type IIS restriction enzymes BsaI and BsmBI. For information on how these parts have been designed, see our design page (link).

    Promoters

    With respect to promoters, we include three constitutive promoters of different forces, a strong promoter for T7 phage DNA polymerase, and a promoter for the sigma 32 subunit of E. coli DNA polymerase. In addition, in our collection there are three promoters regulated by a transcription factor: the inducible pBad, positively regulated by AraC in the presence of L-arabinose, and the inducible and repressible lux promoters, positively and negatively regulated by LuxR in the presence of Acyl-homoserine-lactone, respectively.

    Part Original BioBrick Description
    BBa_K2656004 BBa_J23106 Constitutive promoter
    BBa_K2656005 BBa_J23102 Constitutive promoter
    BBa_K2656007 BBa_J23101 Constitutive promoter
    BBa_K2656000 BBa_I719005 Strong promoter for T7 phage DNA polymerase
    BBa_K2656001 BBa_K338001 Promoter for the sigma 32 subunit of E. coli DNA polymerase
    BBa_K2656006 BBa_K2442101 pBad minimal promoter: positively regulated by AraC in the presence of L-arabinose
    BBa_K2656002 BBa_R0061 Lux repressible promoter: negatively regulated by LuxR in the presence of Acyl-homoserine-lactone.
    BBa_K2656003 BBa_R0062 Lux inducible promoter: positively regulated by LuxR in the presence of Acyl-homoserine-lactone.

    RBS

    Regarding the RBS, we have selected five from the Registry of Standard Biological Parts, with different strengths.

    Part Original BioBrick Description
    BBa_K2656008 BBa_J61100 Very weak RBS
    BBa_K2656009 BBa_B0030 Strong RBS
    BBa_K2656010 BBa_B0032 Weak RBS
    BBa_K2656011 BBa_B0034 Medium RBS
    BBa_K2656012 BBa_J61101 Very weak RBS

    CDS

    For CDS we have chosen different coding sequences of reporter proteins: fluoroproteins (sfGFP, mRFP1, YFP and GFPmut3b) and chromoproteins (amilCP). Another choice we have made is the addition of the LVA degradation tag to the mRFP1, YFP and GFPmut3b sequences. We have also selected the BSMT1 enzyme coding sequence that will allow our bacteria to smell of mint in the presence of salicylic acid. Additionally, we have added to the collection the CDS of the transcription factors AraC and LuxR that act on our regulable promoters, as well as the CDS of LuxI that acts producing the Acyl-Homoserine-Lactone to which LuxR responds. For this last CDS, we have codon optimized its sequence for E. Coli. Finally, we have picked a lysis gene from the enterobacteria phage phiX174 which allows us to create genetic circuits whose functionality implies the death of part of the population. All these coding sequences are codon optimized for E. coli.

    Part Original BioBrick Description
    BBa_K2656013 BBa_I746916 Superfolder Green fluorescent Protein (sfGFP) coding sequence
    BBa_K2656014 BBa_E1010 monomeric Red fluorescent protein (mRFP1) coding sequence
    BBa_K2656021 BBa_K592101 Yellow fluorescent protein (YFP) coding sequence
    BBa_K2656022 BBa_E0040 Green fluorescent protein (GFPmut3b) coding sequence
    BBa_K2656018 BBa_K592009 amilCP (Blue chromoprotein) coding sequence
    BBa_K2656024 BBa_K1399001 mRFP1 with the LVA tag coding sequence
    BBa_K2656020 None YFP with the LVA tag coding sequence
    BBa_K2656023 BBa_K1399004 GFPmut3b with the LVA tag coding sequence
    BBa_K2656025 BBa_J45004 BSMT1 coding sequence
    BBa_K2656017 BBa_K2442103 AraC transcription factor coding sequence
    BBa_K2656016 BBa_C0062 LuxR transcription factor coding sequence
    BBa_K2656019 BBa_C0161 LuxI (Acyl-Homoserine-Lactone synthase) codon optimized coding sequence
    BBa_K2656015 None Lysis gene from the enterobacteria phage phiX174

    Transcriptional terminators

    Lastly, as far as terminators are concerned, we have only chosen one, as we do not think that introducing several would have an influence on the functioning of Printeria. The terminator chosen is B0015 because it is the most used in E. Coli.

    Part Original BioBrick Description
    BBa_K2656026 BBa_B0015 Double transcriptional terminator

    Composite Parts

    In order to make the necessary measurements to characterize the basic parts and demonstrate that they are functional, we have built some composite parts by combining a promoter, an RBS, a CDS and the terminator in a GoldenBraid Alpha 1 vector (link a GoldenBraid 3.0) with the Golden Gate assembly protocol (link a protocolos).

    Composite Part PromoterRBSCDSTerminatorDescription
    BBa_K2656100BBa_K2656004BBa_K2656008BBa_K2656013BBa_K2656026sfGFP transcriptional unit 1
    BBa_K2656101BBa_K2656004BBa_K2656009BBa_K2656013BBa_K2656026sfGFP transcriptional unit 2
    BBa_K2656102BBa_K2656004BBa_K2656010BBa_K2656013BBa_K2656026sfGFP transcriptional unit 3
    BBa_K2656103BBa_K2656004BBa_K2656011BBa_K2656013BBa_K2656026sfGFP transcriptional unit 4
    BBa_K2656104BBa_K2656004BBa_K2656012BBa_K2656013BBa_K2656026sfGFP transcriptional unit 5
    BBa_K2656105BBa_K2656004BBa_K2656009BBa_K2656022BBa_K2656026GFP transcriptional unit 1
    BBa_K2656106BBa_K2656005BBa_K2656009BBa_K2656022BBa_K2656026GFP transcriptional unit 2
    BBa_K2656107BBa_K2656007BBa_K2656009BBa_K2656022BBa_K2656026GFP transcriptional unit 3
    BBa_K2656117BBa_K2656004BBa_K2656011BBa_K2656022BBa_K2656026GFP transcriptional unit 4
    BBa_K2656108BBa_K2656004BBa_K2656009Bba_K2656023BBa_K2656026GFP_LVA transcriptional unit
    BBa_K2656109BBa_K2656004BBa_K2656009Bba_K2656014BBa_K2656026mRFP1 transcriptional unit 1
    BBa_K2656118BBa_K2656001BBa_K2656009BBa_K2656022BBa_K2656026mRFP1 transcriptional unit 2
    BBa_K2656119BBa_K2656002BBa_K2656009BBa_K2656022BBa_K2656026mRFP1 transcriptional unit 3
    BBa_K2656110BBa_K2656004BBa_K2656009Bba_K2656024BBa_K2656026mRFP1_LVA transcriptional unit 1
    BBa_K2656111BBa_K2656004BBa_K2656009BBa_K2656021BBa_K2656026YFP_LVA transcriptional unit 1
    BBa_K2656112BBa_K2656004BBa_K2656009BBa_K2656018BBa_K2656026YFP transcriptional unit 1
    BBa_K2656113BBa_K2656004BBa_K2656009BBa_K2656016BBa_K2656026amilCP transcriptional unit 1
    BBa_K2656120BBa_K2656004BBa_K2656011BBa_K2656018BBa_K2656026amilCP transcriptional unit 2
    BBa_K2656115BBa_K2656001BBa_K2656009BBa_K2656022BBa_K2656026HSP-GFPmut3b transcriptional unit
    BBa_K2656114BBa_K2656004BBa_K2656009BBa_K2656016BBa_K2656026Constitutive LuxR transcriptional unit
    BBa_K2656116BBa_K2656002BBa_K2656009BBa_K2656022BBa_K2656026pLuxR-GFPmut3b transcriptional unit
    BBa_K2656122BBa_K2656003BBa_K2656011BBa_K2656022BBa_K2656026pLux-GFPmut3b transcriptional unit
    BBa_K2656125BBa_K2656004BBa_K2656009BBa_K2656019BBa_K2656026Constitutive LuxI transcriptional unit 1
    BBa_K2656123BBa_K2656000BBa_K2656010BBa_K2656022BBa_K2656026pT7-GFPmut3b transcriptional unit 1
    BBa_K2656124BBa_K2656000BBa_K2656011BBa_K2656022BBa_K2656026pT7-GFPmut3b transcriptional unit 2
    BBa_K2656121BBa_K2656004BBa_K2656011BBa_K2656017BBa_K2656026AraC constitutive transcriptional unit
    BBa_K2656126BBa_K2656004BBa_K2656009BBa_K2656025BBa_K2656026BMSTI transcriptional unit

    Plasmids

    Our transcriptional units were built using a GoldenBraid 3.0 alpha 1 vector which is not compatible with the BioBrick grammar. Therefore, we have created the BBa_K2656200 plasmid, adapted from the standard pSB1C3 backbone, to convert our Golden Braid transcriptional units to BioBricks.

    BBa_K2656200 is a modified version of BBa_P10500, so it includes BsmBI restriction sites flanking a RFP selection marker. When digesting this plasmid with BsmBI the result is complementary sticky ends to those formed in a Golden Braid alpha 1 plasmid when digesting with BsmBI. By this way, the BioBrick compatibility for each composite part in our collection is easily achieved using this plasmid as the destination vector in a Golden Gate reaction with the BsmBI endonuclease.

    Part Description
    BBa_K2656200 BioBricks compatible plasmid

    CONTACT US igem.upv.2018@gmail.com