Difference between revisions of "Team:Worldshaper-XSHS/Demonstrate"

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                     <ul class="colul text-left"style="width: 180px;font-size: 15px!important">
 
                     <ul class="colul text-left"style="width: 180px;font-size: 15px!important">
                         <li class="colactive mt-1"><a href="#Ourteam">Collaboration with ASTWS-China team and HFLS_ZhejiangUnited team.</a></li>
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                         <li class="colactive mt-1"><a href="#Ourteam">the first generation product.</a></li>
 
                         <hr>
 
                         <hr>
                         <li class="mt-1"><a href="#OurSchool">Collaboration with ZJU-China team.</a></li>
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                         <li class="mt-1"><a href="#OurSchool">the second generation product.</a></li>
                        <hr>
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                        <li class="mt-1"><a href="#Others">Collaborations with social organizations.</a></li>
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                         "></div>
 
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                         <p style="width: 800px;margin-left: 11px;margin-top: -23px;font-size: 22px;margin-bottom: 20px;">Collaboration with ASTWS-China team and HFLS_ZhejiangUnited team.</p>
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                         <p style="width: 800px;margin-left: 11px;margin-top: -23px;font-size: 22px;margin-bottom: 20px;">The first generation product.</p>
 
                     </div>
 
                     </div>
                     
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                    <div class="xshs-box2">
 
                         <p class="itemstyle">
 
                         <p class="itemstyle">
                            During the interlab experiment, we cooperated with ASTWS-China team and HFLS_ZhejiangUnited team, we provided the ASTWS-China team with E. coli DH5a and 100μl silicon beads that had better quality as well as gave HFLS_ZhejiangUnited team the transcriptional shaking table bacteria solution and some experimental equipment (chloramphenicol resistance, gun head, etc.). At the same time, we also borrowed the glycerin bacteria we needed from the ASTWS-China team.
+
                          According to the results of the survey and interview, we designed two generations of products. Due to limited time, we only made models of the first generation product. The design of the second generation model has been improved based on the first generation, which makes up for the deficiency of the first generation.
 
                         </p>
 
                         </p>
                    </div>
 
                    <div class="xshs-box2">
 
                        <p>
 
                            <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/a/ab/T--worldshaper-XSHS--c001.png" />
 
                        </p>
 
                    </div>
 
                    <div class="xshs-box3">
 
 
                         <p class="itemstyle">
 
                         <p class="itemstyle">
                            During the experiment, we lent ASTWS-China team kit1 and kit2, which enables its students to extract the useful DNA.
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                          The first generation product was a stationary nicotine testing device (see wiki). The instrument is divided into three parts: air inlet pipe, box body and air outlet pipe. The chamber is filled with bacteria liquid. A fan is used to extract the upper part of the gas to form a low-pressure area. The atmospheric pressure will pump the gas to be tested from the other side into the inlet pipe, which is bent down and connected to the bottom of the device, so that the gas can fully contact with the liquid and water-soluble nicotine can be transferred from the air into the bacteria liquid. After that, we got the bacterial fluid that expressed the fluorescent protein under the effect of nicotine, measured the fluorescence, and calculated the nicotine concentration.
 
                         </p>
 
                         </p>
 
                         <p class="itemstyle">
 
                         <p class="itemstyle">
                            During the Collaborations, our research members visited HFLS_ZhejiangUnited team’s experiments and watched its members’ studying notes.
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                          We used 3D printing technology to construct our product design into a physical model. The main body of the product model is a cuboid of 15cm in length, 7cm in width and 7cm in height. (see below). The air inlet and outlet with diameter of 2cm are also provided.
 
                         </p>
 
                         </p>
 
                     </div>
 
                     </div>
 
                     <div class="xshs-box2">
 
                     <div class="xshs-box2">
 
                         <p>
 
                         <p>
                             <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/3/35/T--worldshaper-XSHS--c002.png" />
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                             <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/f/f8/T--Worldshaper-XSHS--de001.png" />
 
                         </p>
 
                         </p>
 
                     </div>
 
                     </div>
 
                     <div class="xshs-box3">
 
                     <div class="xshs-box3">
 
                         <p class="itemstyle">
 
                         <p class="itemstyle">
                            Because our conversion process——A procedure that obtaining the desired plasmid from the distribution kits—— always failed,we communicated with ASTWS-China team, eliminating the possibilities derived from operate miss and finding the cause of the problem: Homemade E. coli receptive state.
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                          In the design of the second generation product (see Wiki), we reduced the device size (as shown in the figure below)-the actual internal bottom area was about 16cm squared-and the amount of bacterial liquid needed to work. The equipment also adopts the pump type piston pumping way, which enable filters device to reduce the possible escherichia coli leakage properly. Because the piston is used to pump air, the gas is a fixed column of air volume when the piston pumps back and forth, bringing great convenience for us to exactly extract the volume we want.
 
                         </p>
 
                         </p>
 
                     </div>
 
                     </div>
 
                     <div class="xshs-box2">
 
                     <div class="xshs-box2">
 
                         <p>
 
                         <p>
                             <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/b/bb/T--worldshaper-XSHS--c003.png" />
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                             <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/5/51/T--Worldshaper-XSHS--de002.png" />
 
                         </p>
 
                         </p>
                    </div>
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                        <p>
 
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                            <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/6/68/T--Worldshaper-XSHS--de003.png" />
                    <div class="xshs-box3">
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                        <p class="v1" id="OurSchool"style="text-align: center; "> Collaborations with other teams</p>
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                            <p style="width: 800px;margin-left: 11px;margin-top: -23px;font-size: 22px;margin-bottom: 20px;">Collaboration with ZJU-China team.</p>
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                        <p class="itemstyle">
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                            The aspect about experimental problem: Because of the invalid homemade E. coli receptive state, we did experiments in the ZJU-China team’s laboratories by utilizing its commercial E. coli receptive state. This attempt provided enough opportunities for us to discuss the difference between the experiments, which exerted great advantage to solve such problems in the nest phase.
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                         </p>
 
                         </p>
                    </div>
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                        <p>
                    <div class="xshs-box2">
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                             <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/1/10/T--Worldshaper-XSHS--de004.png" />
                        <p>
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                            <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/7/74/T--worldshaper-XSHS--c004.png" />
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                    <div class="xshs-box3">
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                        <p class="itemstyle">
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                            Before carrying out publicity activities, our team made comprehensive discussions with the ZJU-China team like establishing programs of activities, preparing materials, and decorating the venues.
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                        </p>
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                    </div>
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                    <div class="xshs-box2">
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                        <p>
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                            <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/e/e1/T--worldshaper-XSHS--c005.png" />
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                    <div class="xshs-box3">
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                        <p class="v1" id="Others"style="text-align: center;">Collaborations with social organizations</p>
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                        <hr style="
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                            background-color: #0c3d0c99!important;
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                        ">
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                        <div class="xshs-box3">
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                            <div style="
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                            height: 24px;
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                            <p style="width: 800px;margin-left: 11px;margin-top: -23px;font-size: 22px;margin-bottom: 20px;">Collaboration with Zhejiang Province Science Museum</p>
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                        </div>
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                        <p class="itemstyle" style="margin-left: 0px;width: 1100;">
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                          We cooperated with Science museum and did some simple experiments in the venue stadium, which is not only appeals visitors, but also facilitates to promote the IGEM project and knowledge about synthetic biology.
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                        </p>
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                        <div class="xshs-box3">
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                            <div style="
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                        "></div>
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                            <p style="width: 800px;margin-left: 11px;margin-top: -23px;font-size: 22px;margin-bottom: 20px;">Collaborations with Zhejiang Science and Technology Market</p>
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                        </div>
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                        <p class="itemstyle" style="margin-left: 0px;width: 1100;">
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                          During the science and technology week held by Zhejing Science administrators, we collaborated with organizers. In that exhibition, we conducted a questionnaire survey as well as advertised the JGEM project, thus, enriching the program’s content.
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                        </p>
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                    </div>
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                    <div class="xshs-box2">
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                        <p>
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                             <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/a/a6/T--worldshaper-XSHS--c006.png" />
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                         </p>
 
                         </p>
 
                     </div>
 
                     </div>

Revision as of 11:02, 16 October 2018

Demonstrate

Collaborations with other teams


The first generation product.

According to the results of the survey and interview, we designed two generations of products. Due to limited time, we only made models of the first generation product. The design of the second generation model has been improved based on the first generation, which makes up for the deficiency of the first generation.

The first generation product was a stationary nicotine testing device (see wiki). The instrument is divided into three parts: air inlet pipe, box body and air outlet pipe. The chamber is filled with bacteria liquid. A fan is used to extract the upper part of the gas to form a low-pressure area. The atmospheric pressure will pump the gas to be tested from the other side into the inlet pipe, which is bent down and connected to the bottom of the device, so that the gas can fully contact with the liquid and water-soluble nicotine can be transferred from the air into the bacteria liquid. After that, we got the bacterial fluid that expressed the fluorescent protein under the effect of nicotine, measured the fluorescence, and calculated the nicotine concentration.

We used 3D printing technology to construct our product design into a physical model. The main body of the product model is a cuboid of 15cm in length, 7cm in width and 7cm in height. (see below). The air inlet and outlet with diameter of 2cm are also provided.

In the design of the second generation product (see Wiki), we reduced the device size (as shown in the figure below)-the actual internal bottom area was about 16cm squared-and the amount of bacterial liquid needed to work. The equipment also adopts the pump type piston pumping way, which enable filters device to reduce the possible escherichia coli leakage properly. Because the piston is used to pump air, the gas is a fixed column of air volume when the piston pumps back and forth, bringing great convenience for us to exactly extract the volume we want.

  • Worldshaper-XSHS, Xiaoshan High School

    Adress: No.538,Gongxiu Road,Xiaoshan District,Hangzhou,Zhejiang Province,China