Part Collection
As we have explained in [página del porqué usamos GB] we are using the Golden Gate assembly method. But we have a problem: There is not a collection of parts as complete as for BioBricks.
In order to solve this problem, we have made a collection of characterized basic parts (promoters, RBS, CDS and terminators), domesticating them to the Golden Braid 3.0 grammar and creating transcriptional units with them.
The grammar of the basic parts is the same as in MoClo but we have selected The Golden Braid 3.0 assembly method because it allows to create multigenic constructions with a simple binary system: the transcriptional units are assembled in only 2 different destination plasmids. [link a Golden braid].
pie de foto
Part Table
We have created these parts:
<groupparts class="col-md-12">iGEM18 Valencia_UPV</groupparts>