Team:Valencia UPV/Safety

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Introduction

As Theodor Reik (Sigmund Freud disciple) said: “In science, like in all life fields, a slogan is imposed: security first”. Nowadays, safety is an essential requirement when creating new devices that humans will use, and Printeria is not different.

This document describes the safety of our project so, a clever step before begining is to know what is it. Safety describes the containment principles, technologies and practices that are implemented to prevent the unintentional exposure to pathogens and toxines, or their accidental release [1]. Furthermore, safety is how you have protected yoursef and other in order to avoid any harms which could occur.

Printeria is designed to be a secure device, because is going to be used by bioartists, schools and researchers (our identified potential users according to ENLACE TABLA DEL MIT) and we don’t want it to be a variable which create concern in those users. This approach toguether with our lab tools and posibilities have influenced the experiments we did and the way we build Printeria. Next paragraphs describe how and why we implemented biosafety and biosecurity requirements in Printeria.

Lab safety

Safety design in Printeria

Lab Safety

Our laboratoy

We have planned this project consequently with the tools we have. Our lab follows the current legislation about risk 1 (Ley 31/1995, RD 664/1997) and as it is written is provided with an autoclave and an extractor hood with UV sterilization. The waste generated are minutely controlled by CGROM ( UPV waste management center ). They prove every lab with special containers and instructions for the correct classification of wate. FOTO CONENEDORES As an additional safety measure we use SYBR Safe instead of Ethidium bromide. Its use is identical in all the protocols but without carcinogenic risks

Access to the laboratory

As we are a multidisciplinary team formed by Biotechnologists, engineers and artists not everybody knows how to behaviour in a lab. Thats why we did several seminars directed by Alejandro Vignoni and Yadira Boada (DOCUMENTOS SEGURIDAD DISA) to teach the basics of their lab. However, not everybody is allowed to work in the lab, only biotechlogistan and Biomedical engineers could be there without surveillance.

Strains

Printeria use E.coli and V.natriegens as chassis. Both are classified as risk 1 so the risk to people is minimal. E.coli are from strains DB3.1, 10G and TOP10, for V.natriegens we use the strain ATCC 1404 from CECT (Colección Española de Cultivos Tipo). To do constructions we use biobricks and parts supplied by IDT prepared with the Golden Gate grammar.

Bibliography

[1] Dual use: A report by iGEM team Bielefeld-CeBiTec 2015. [Germany]. Universität Bielefeld, . 30 pages.

CONTACT US igem.upv.2018@gmail.com