Team:Valencia UPV/Results

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Goals

At the start of the summer, the different members of the Valencia_UPV team set themselves objectives in their respective areas to promote the automation of the cloning process and thus bring synthetic biology closer to the people.

Wet Lab

  • Create a standardized Golden Gate basic Parts Collection to supply Printeria with the DNA toolkit to assemble composite parts.

  • Provide Printeria with the best way to transform competent bacteria with the highest transformation efficiency rate.

  • Avoid the plate colony screening step in order to eliminate the necessity of the researcher visual check, so easing Printeria automation.

Hardware

  • Integrate multiple lab equipment around an automated friendly form.

  • Design an easy way of handling a big variety of input liquids providing a straightforward integration with Printeria.

  • Design an easy to use way of handling a big variety of input liquids with easy integration of the machine.

  • Keep the footprint of the machine as small as possible.

  • Allow for maximum flexibility on the use of the machine.

Software

  • Develop a software that allows the user to control the device.

  • Allow the user to design a transcriptional unit with the software.

  • Allow the user to design multi-genetic constructions with the software.

  • Get non-professional users to print basic genetic circuit.

  • Fill out a “recipe” repository with at least 20 recipes.

  • Make a user friendly interface to interact with the software.

  • Control the device’s inventory through the software.

  • Simulate the bacterial growth and the protein expression.

Modeling

  • Design simple mathematical models based which allows us to simulate the different Printeria genetic circuits.

  • Optimize model parameters to match simulation results to experimental data obtained from Printeria.

  • Characterize the parts of our Part Collection from the optimization results and provide the user with all the information about the Printeria kit.

  • Develop a Simulation Tool that allows the user to visualize a prediction of the results of their experiment before running it in Printeria.

CONTACT US igem.upv.2018@gmail.com