Our Blueprint

Aeromonas hydrophila is an ubiquitous gram-negative opportunistic pathogen in aquaculture. Every year, it causes a variety of diseases in fish. The symptoms include ulcers, fin rot, and hemorrhagic septicaemia. When A. hydrophila enters fish body, it often colonise in the gastrointestinal tract first. Its Type II, Type III, and Type VI secretion systems control several virulence factors, including aerolysin cytotoxic enterotoxin (Act) and biofilm formation ability. The secretion systems are further controlled by N-acylhomoserine lactone (AHL)-dependent quorum-sensing system based on the ahyRI locus. A. hydrophila infection is treated by broad-spectrum antibiotics currently. However, the pathogen has developed resistance to most common antibiotics, resulting ineffective treatment. Thus, our project targets to develop an A. hydrophila killer by engineering the fish probiotics, Escherichia coli MG1655. The killer expresses lactonase to degrade quorum sensing signals from the pathogen in aim of reducing the production of virulence factors. Also, it expresses antimicrobial peptides (AMPs) to inhibit the growth of A. hydrophila directly. We plan to regulate lactonase and AMPs expression by using Prhl promoter, which is induced by the pathogen’s dominant quorum sensing molecule, C4-HSL. In addition, we want to hide our killer into capsules. When fish eat the capsules in feed, the killer can arrive at its gastrointestinal tract and protect its health.

Before you start

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Styling your wiki

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Wiki template information

We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!

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Tips

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State your accomplishments! Tell people what you have achieved from the start.
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You have a global audience! Consider the different backgrounds that your users come from.
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Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
Have lots of fun!

Inspiration

You can also view other team wikis for inspiration! Here are some examples:

Team:BNDS CHINA