Team:TU Darmstadt/Composite Part


Best Composite Part Special Prize

For this award we nominate our part BBa_K2770002 because for that particular part we were able to show protein production via Western Blot, were able to purify it using an Äkta, showed in vitro protein activity and product yield using an HPLC. For further information on the particular results please visit the respective pages on our wiki and the registry. //Comment den ich gerne schreiben würde, aber vlt lieber lassen:// Actually, we could show the mentioned characterizations for all composite parts except the ICL1 expression cassette BBa_K2770007, but for our nominated part we did the experiments the most often with the same positive results and it is the first in our list.

Composite Parts

In the following table are all composite parts listed that we produced this year and submitted to the registry.

part number part type produced enzyme production organism
BBa_K2770002 Generator YcdW - E. coli glyoxylate reductase E. coli
BBa_K2770003 Generator AceA - E. coli isocitrate lyase E. coli
BBa_K2770006 Generator Glyr1 - A. thaliana glyoxylate reductase 1 S. cerevisae
BBa_K2770007 Generator ICL1 - S. cerevisae isocitrate lyase 1 S. cerevisae
BBa_K2770010 Generator CHMO - A. calcoaceticus cyclohexanone monooxygenase E. coli
BBa_K2770011 Generator ADH - L. kefiri alcohol dehydrogenase E. coli



Additionally, here are the basic parts listed, which have been used for correct assembly of the composite parts. Therefore, none of these basic parts has been submitted to the registry by us. Some of them are designed by other teams as indicated in the table.

part number part type short description source organism designed by
BBa_K2770000 cds ycdW - glyoxylate reductase E. coli TU_Darmstadt 2018
BBa_K2770001 cds aceA - isocitrate lyase E. coli TU_Darmstadt 2018
BBa_K2770004 cds glyr1 - glyoxylate reductase A. thaliana TU_Darmstadt 2018
BBa_K2770005 cds icl1 - isocitrate lyase S. cerevisae TU_Darmstadt 2018
BBa_K2770008 cds chmo - cyclohexanone monooxygenase A. calcoaceticus TU_Darmstadt 2018
BBa_K2770009 cds adh - alcohol dehydrogenase L. kefiri TU_Darmstadt 2018
BBa_K2770012 protein domain GS-Linker GGSGG - TU_Darmstadt 2018
BBa_K2770013 Affinity-Tag 10x His-Tag - TU_Darmstadt 2018
BBa_K2770014 Linker SA Linker - TU_Darmstadt 2018
BBa_K2770015 Affinity-Tag Strep-Tag II - TU_Darmstadt 2018
BBa_K2770016 DNA AAA sequence for correct assembly - TU_Darmstadt 2018
BBa_J70593 DNA Startcodon - Knight Lab 2010
BBa_K2389005 Restriction Site Kpn1 restriction site - UAlberta 2017
BBa_KM31383 Restriction Site Nco1 restriction site - Instructors MIT 2007
BBa_G0000 DNA SpeI/XbaI Scar - iGEM 2007
BBa_M36117 DNA Stop-Codon - Stanford 2012
BBa_K921000 Promotor T7/Lac Promotor T7 bacteriophage Carnegie_Mellon 2012
BBa_B0034 RBS RBS E. coli Antiquity 2003