Difference between revisions of "Team:TU Darmstadt/InterLab"

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Table 1. Absorbance measurement of LUDOX-S40 and H<sup>2</sup>O of four different replicates.
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Table 1. Absorbance measurement of LUDOX-S40 and H<sub>2</sub>O of four different replicates.
  
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[[image:T--TU_Darmstadt--Interlab1.jpg|200px |alt=Text]]
  
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Figure 1. Data exported from table 1 in beam chart.
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Figure 1. Data from table 1 exported in beam chart.
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<b>Particle Standard Curve</b>
 
<b>Particle Standard Curve</b>
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[[image:T--TU_Darmstadt--Interlab2.jpg|200px |alt=Text]]
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Table 2.
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[[image:T--TU_Darmstadt--Interlab3.jpg|200px |alt=Text]]
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Figure 2.
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[[image:T--TU_Darmstadt--Interlab4.jpg|200px |alt=Text]]
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Figure 3.
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====Fluorescein Standard Curve====
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[[image:T--TU_Darmstadt--Interlab5.jpg|200px |alt=Text]]
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Table 3. Fluorescence measurement of fluorescein in eleven dilutions and one control containing PBS. Measurement settings for the plate reader: Excitation 485 nm, Emission 530 nm, 50 gains and 20 flashes.
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[[image:T--TU_Darmstadt--Interlab6.jpg|200px |alt=Text]]
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Figure 4. Calibration curve of fluorescein: fluorescence measurement of different concentrations (µM).
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[[image:T--TU_Darmstadt--Interlab5.jpg|200px |alt=Text]]
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Figure 5. Calibration curve of fluorescein (log scale): fluorescence measurement of different concentrations (µM).
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===Cell Measurement===

Revision as of 18:07, 4 October 2018

Interlab Study

Since iGEM is an international competition with many teams from all over the world, it is inevitably coping with the problem of unreproducible scientific measurement results. Multiple teams are measuring fluorescence differently, in different units and are evaluating the results non-identically. Therefore, the InterLab Study aims to develop a standardized protocol for repeatable fluorescence measurements of GFP. The study should be carried out and examined in different laboratories all over the world. Our team followed the standardized procedure guiding us through transformation, inoculation and measurement process. For more information: Fifth International InterLab Measurement Study Following below you can find our results.

Calibration

OD600 Calibration

header 1 LUDOX CL-X H2
Replicate 1 0.0525 0.0355
Replicate 2 0.0526 0.0351
Replicate 3 0.0529 0.0373
Replicate 4 0.0528 0.0352
Arithmetic Mean 0.0528 0.0358
Corrected Absorbance 600 0.0169
Reference OD600 0.0630
Correction Factor 3.7223

Table 1. Absorbance measurement of LUDOX-S40 and H2O of four different replicates.

Text

Figure 1. Data exported from table 1 in beam chart.


Particle Standard Curve Text Table 2.

Text Figure 2.

Text Figure 3.

Fluorescein Standard Curve

Text Table 3. Fluorescence measurement of fluorescein in eleven dilutions and one control containing PBS. Measurement settings for the plate reader: Excitation 485 nm, Emission 530 nm, 50 gains and 20 flashes.

Text Figure 4. Calibration curve of fluorescein: fluorescence measurement of different concentrations (µM).

Text Figure 5. Calibration curve of fluorescein (log scale): fluorescence measurement of different concentrations (µM).


Cell Measurement