Team:TU Darmstadt/InterLab
Interlab Study
Since iGEM is an international competition with many teams from all over the world, it is inevitably coping with the problem of unreproducible scientific measurement results. Multiple teams are measuring fluorescence differently, in different units and are evaluating the results non-identically. Therefore, the InterLab Study aims to develop a standardized protocol for repeatable fluorescence measurements of GFP. The study should be carried out and examined in different laboratories all over the world. Our team followed the standardized procedure guiding us through transformation, inoculation and measurement process. For more information: Fifth International InterLab Measurement Study Following below you can find our results.
Calibration
OD600 Calibration
| header 1 | LUDOX CL-X | H2 |
|---|---|---|
| Replicate 1 | 0.0525 | 0.0355 |
| Replicate 2 | 0.0526 | 0.0351 |
| Replicate 3 | 0.0529 | 0.0373 |
| Replicate 4 | 0.0528 | 0.0352 |
| Arithmetic Mean | 0.0528 | 0.0358 |
| Corrected Absorbance 600 | 0.0169 | |
| Reference OD600 | 0.0630 | |
| Correction Factor | 3.7223 |
Table 1. Absorbance measurement of LUDOX-S40 and H2O of four different replicates.