Line 3: | Line 3: | ||
#top-bar.animated-header{ | #top-bar.animated-header{ | ||
padding: 20px 0; | padding: 20px 0; | ||
− | background: #09b0ff; | + | background: #09b0ff!important; |
box-shadow: none; | box-shadow: none; | ||
} | } |
Revision as of 11:18, 5 October 2018
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report.
Abstract