Difference between revisions of "Team:Worldshaper-XSHS"
| Line 76: | Line 76: | ||
<script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.fancybox.js"></script> | <script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.fancybox.js"></script> | ||
<script src="http://igem2017.worldshaper.cn/XSHS/js/main.js"></script> | <script src="http://igem2017.worldshaper.cn/XSHS/js/main.js"></script> | ||
| − | <header id="top-bar" class="navbar-fixed-top animated-header navbar navbar-default" | + | <header id="top-bar" class="navbar-fixed-top animated-header navbar navbar-default myheader" > |
<div class="container"> | <div class="container"> | ||
<div class="navbar-header" > | <div class="navbar-header" > | ||
Revision as of 12:19, 8 October 2018
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for
public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute
Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene,
a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate,
nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
to sense the phosphate concentration and a RFP gene to report.
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report.
Abstract
Abstract
