Difference between revisions of "Team:Worldshaper-XSHS"
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<section id="about" style="background:#fff"> | <section id="about" style="background:#fff"> | ||
<div class="container"> | <div class="container"> | ||
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<div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | ||
| − | <h2>Abstract</h2> | + | <h2 class"v1">Abstract</h2> |
| − | <p> | + | <p class="itemstyle"> |
| − | + | Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water | |
| + | pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating | ||
| + | and low-cost tool for public to monitor water quality around. | ||
</p> | </p> | ||
| − | <p> | + | <p class="itemstyle"> |
| − | We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report. | + | We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved |
| + | oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP | ||
| + | reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a | ||
| + | vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter | ||
| + | was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter | ||
| + | gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the | ||
| + | phosphate concentration and a RFP gene to report. | ||
</p> | </p> | ||
</div> | </div> | ||
| − | + | <div> | |
| + | <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/f/f1/T-worldshaper-XSHS-k010.png" alt=""> | ||
| + | </div> | ||
| + | </div> | ||
</div> | </div> | ||
| − | <div class="col-md-6 col-sm-6"> | + | <div class="row"> |
| − | <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;"> | + | <div class="col-md-6 col-sm-6"> |
| − | + | <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | |
| + | <h2 class"v1">Abstract</h2> | ||
| + | <p class="itemstyle"> | ||
| + | Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water | ||
| + | pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating | ||
| + | and low-cost tool for public to monitor water quality around. | ||
| + | </p> | ||
| + | <p class="itemstyle"> | ||
| + | We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved | ||
| + | oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a | ||
| + | GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains | ||
| + | a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR | ||
| + | promoter was used to response to different concentrations of nitrate, nitrite and nitric, with | ||
| + | a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ | ||
| + | to sense the phosphate concentration and a RFP gene to report. | ||
| + | </p> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | <div class="col-md-6 col-sm-6"> | ||
| + | <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/f/f1/T-worldshaper-XSHS-k010.png" alt=""> | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
</section> | </section> | ||
Revision as of 12:42, 8 October 2018
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP
reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a
vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter
was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter
gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the
phosphate concentration and a RFP gene to report.
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a
GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains
a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR
promoter was used to response to different concentrations of nitrate, nitrite and nitric, with
a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
to sense the phosphate concentration and a RFP gene to report.
Abstract
Abstract
