Line 35: | Line 35: | ||
} | } | ||
.colul li a{ | .colul li a{ | ||
− | font-family: | + | font-family: purista !important; |
font-weight: 400; | font-weight: 400; | ||
font-style: normal; | font-style: normal; | ||
Line 68: | Line 68: | ||
background:rgba(0,0,0,1) !important; | background:rgba(0,0,0,1) !important; | ||
} | } | ||
− | + | @font-face | |
+ | { | ||
+ | font-family: purista; | ||
+ | src: url('https://static.igem.org/mediawiki/2018/4/44/T--worldshaper-XSHS--semibold.otf'), | ||
+ | url('https://static.igem.org/mediawiki/2018/4/44/T--worldshaper-XSHS--semibold.otf'); | ||
+ | } | ||
</style> | </style> | ||
<link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/wiki_edit.css"> | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/wiki_edit.css"> |
Revision as of 07:16, 10 October 2018
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP
reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a
vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter
was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter
gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the
phosphate concentration and a RFP gene to report.
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a
GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains
a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR
promoter was used to response to different concentrations of nitrate, nitrite and nitric, with
a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
to sense the phosphate concentration and a RFP gene to report.
Abstract
Abstract