Difference between revisions of "Team:Worldshaper-XSHS"
| Line 79: | Line 79: | ||
{ | { | ||
font-family: michroma; | font-family: michroma; | ||
| − | src: url('https://static.igem.org/mediawiki/2018/ | + | src: url('https://static.igem.org/mediawiki/2018/2/26/T--worldshaper-XSHS--michroma.otf'), |
| − | url('https://static.igem.org/mediawiki/2018/4/41/T--worldshaper-XSHS--michroma. | + | url('https://static.igem.org/mediawiki/2018/4/41/T--worldshaper-XSHS--michroma.otf'); |
} | } | ||
</style> | </style> | ||
Revision as of 13:04, 10 October 2018
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP
reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a
vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter
was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter
gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the
phosphate concentration and a RFP gene to report.
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a
GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains
a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR
promoter was used to response to different concentrations of nitrate, nitrite and nitric, with
a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
to sense the phosphate concentration and a RFP gene to report.
Abstract
Abstract
