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top:200px; | top:200px; | ||
font-size: 68px; | font-size: 68px; | ||
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letter-spacing: 0.0588235em; | letter-spacing: 0.0588235em; | ||
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.colul .colactive a{ | .colul .colactive a{ | ||
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color: #00746b !important; | color: #00746b !important; | ||
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font-weight: 600 !important; | font-weight: 600 !important; | ||
font-style: normal !important; | font-style: normal !important; | ||
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margin-bottom: .5em; | margin-bottom: .5em; | ||
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line-height: 1.6em !important; | line-height: 1.6em !important; | ||
color: rgba(26,26,26,.7) !important; | color: rgba(26,26,26,.7) !important; | ||
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<div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | ||
<h2 class="v1">Abstract</h2> | <h2 class="v1">Abstract</h2> | ||
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+ | margin: 0 auto; | ||
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<p class="itemstyle"> | <p class="itemstyle"> | ||
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water | Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water |
Revision as of 12:12, 11 October 2018
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP
reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a
vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter
was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter
gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the
phosphate concentration and a RFP gene to report.
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a
GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains
a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR
promoter was used to response to different concentrations of nitrate, nitrite and nitric, with
a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
to sense the phosphate concentration and a RFP gene to report.
Abstract
Abstract