Line 70: | Line 70: | ||
text-align:left; | text-align:left; | ||
} | } | ||
− | + | ||
− | + | ||
− | + | ||
.footer{ | .footer{ | ||
background:rgba(12, 61, 12, 0.8509803921568627) !important; | background:rgba(12, 61, 12, 0.8509803921568627) !important; | ||
Line 248: | Line 246: | ||
<hr style=" | <hr style=" | ||
height: 3px; | height: 3px; | ||
+ | background-color: #0c3d0c99!important | ||
"> | "> | ||
</div> | </div> | ||
Line 275: | Line 274: | ||
<div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | ||
<h2 class="v1">Abstract</h2> | <h2 class="v1">Abstract</h2> | ||
+ | |||
+ | <div style=" | ||
+ | width: 100%; | ||
+ | margin-left: 0; | ||
+ | margin-top: -34px; | ||
+ | margin-bottom: -20px; | ||
+ | "> | ||
+ | <hr style=" | ||
+ | height: 3px; | ||
+ | background-color: #0c3d0c99!important | ||
+ | width: 97%; | ||
+ | "> | ||
+ | <p style=" | ||
+ | margin-top: -18px; | ||
+ | font-size: 13px; | ||
+ | line-height: 20px; | ||
+ | font-family: ration cans; | ||
+ | " align="left">We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. </p> | ||
+ | </div> | ||
+ | |||
<p class="itemstyle"> | <p class="itemstyle"> | ||
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water | Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water |
Revision as of 12:16, 11 October 2018
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP
reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a
vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter
was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter
gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the
phosphate concentration and a RFP gene to report.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively.
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a
GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains
a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR
promoter was used to response to different concentrations of nitrate, nitrite and nitric, with
a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
to sense the phosphate concentration and a RFP gene to report.
Abstract
Abstract