Difference between revisions of "Team:Worldshaper-XSHS"
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<section id="about" style="background:rgba(242, 241, 241, 0.75)"> | <section id="about" style="background:rgba(242, 241, 241, 0.75)"> | ||
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| + | <div class="col-md-6 col-sm-6"> | ||
| + | <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | ||
| + | <h2 class="v1">Abstract</h2> | ||
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| + | " align="left">We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. </p> | ||
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| + | <p class="itemstyle"> | ||
| + | Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water | ||
| + | pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating | ||
| + | and low-cost tool for public to monitor water quality around. | ||
| + | </p> | ||
| + | <p class="itemstyle"> | ||
| + | We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved | ||
| + | oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a | ||
| + | GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains | ||
| + | a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR | ||
| + | promoter was used to response to different concentrations of nitrate, nitrite and nitric, with | ||
| + | a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ | ||
| + | to sense the phosphate concentration and a RFP gene to report. | ||
| + | </p> | ||
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| + | </div> | ||
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| + | <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/f/f1/T-worldshaper-XSHS-k010.png" alt=""> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="row text-center"> | ||
<div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | ||
<h2 class="v1">Abstract</h2> | <h2 class="v1">Abstract</h2> | ||
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<div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;"> | <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;"> | ||
<img src="https://static.igem.org/mediawiki/2017/f/f1/T-worldshaper-XSHS-k010.png" alt=""> | <img src="https://static.igem.org/mediawiki/2017/f/f1/T-worldshaper-XSHS-k010.png" alt=""> | ||
Revision as of 03:40, 13 October 2018
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively.
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a
GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains
a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR
promoter was used to response to different concentrations of nitrate, nitrite and nitric, with
a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
to sense the phosphate concentration and a RFP gene to report.
It's better to quit smoking.
China is not only the world's largest tobacco producer, but also the
world's largest tobacco consumer.
As we all know, smoking is extremely harmful to health, and secondhand
smoke is very easy to cause damage to public health. In daily life, people
have been suffering a lot.
Nicotine, which stems from ignited cigarettes, is so addictive that it is a
sort of aromatic poison to smokers.
Through literature reviewing, it is found that nicotine has adverse effects
on many tissues or organs of human body and is a major threat to
human health.
At the same time, the high content of nicotine in tobacco-leave waste
will pose a serious threat to the safety of groundwater in China.
Scholars at home and abroad have been paying attention to the
degradation of nicotine by microorganisms. Comparing with physical
and chemical methods, microbial degradation of nicotine is more
economical, more efficient and more sustainable.
Abstract
Abstract
