Difference between revisions of "Team:Worldshaper-XSHS"
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<link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/wiki_edit.css"> | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/wiki_edit.css"> | ||
<link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/bootstrap.min.css"> | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/bootstrap.min.css"> | ||
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<script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.fancybox.js"></script> | <script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.fancybox.js"></script> | ||
<script src="http://igem2017.worldshaper.cn/XSHS/js/main.js"></script> | <script src="http://igem2017.worldshaper.cn/XSHS/js/main.js"></script> | ||
| − | + | <header id="top-bar" class="navbar-fixed-top animated-header navbar navbar-default myheader" > | |
<div class="container"> | <div class="container"> | ||
| − | <div class="navbar-header"> | + | <div class="navbar-header" > |
<button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-collapse"> | <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-collapse"> | ||
<span class="sr-only">Toggle navigation</span> | <span class="sr-only">Toggle navigation</span> | ||
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<span class="icon-bar"></span> | <span class="icon-bar"></span> | ||
<span class="icon-bar"></span> | <span class="icon-bar"></span> | ||
| − | + | </button> | |
| − | <div class="navbar-brand"> | + | <div class="navbar-brand " style="display:block;color:#fff;margin-left:15px;font-size:20px"> |
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS"> |
| − | + | Worldshaper- <b>XSHS</b> | |
</a> | </a> | ||
</div> | </div> | ||
| − | + | </div> | |
<nav class="collapse navbar-collapse navbar-right" role="navigation"> | <nav class="collapse navbar-collapse navbar-right" role="navigation"> | ||
<div class="main-menu"> | <div class="main-menu"> | ||
<ul class="nav navbar-nav navbar-right"> | <ul class="nav navbar-nav navbar-right"> | ||
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<li class="dropdown"> | <li class="dropdown"> | ||
<a class="dropdown-toggle" data-toggle="dropdown"> | <a class="dropdown-toggle" data-toggle="dropdown"> | ||
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<div class="dropdown-menu"> | <div class="dropdown-menu"> | ||
<ul> | <ul> | ||
| + | |||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Team">Members</a> |
| − | + | ||
| − | + | ||
| − | + | ||
</li> | </li> | ||
| + | |||
</ul> | </ul> | ||
</div> | </div> | ||
</li> | </li> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Collaborations">Collaborations</a> | ||
| + | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
<a class="dropdown-toggle" data-toggle="dropdown"> | <a class="dropdown-toggle" data-toggle="dropdown"> | ||
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<ul> | <ul> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Background.html">Background</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/design.html">Design</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Protocols-methods.html">Protocols and methods</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/result.html">Results</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a> |
</li> | </li> | ||
| + | |||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Model">Model</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Demonstrate">Demonstrate</a> |
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| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/parts.html">Parts</a> |
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
<a class="dropdown-toggle" data-toggle="dropdown"> | <a class="dropdown-toggle" data-toggle="dropdown"> | ||
| − | + | HP | |
<span class="caret"></span> | <span class="caret"></span> | ||
</a> | </a> | ||
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<ul> | <ul> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/HP/Silver">Silver</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/HP/Gold_Integrated">Integrated and Gold</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Engagement">Engagement</a> |
</li> | </li> | ||
</ul> | </ul> | ||
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</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Applied_Design">Applied Design</a> |
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
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<ul> | <ul> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Medal-requirements.html">Medal requirements</a> |
</li> | </li> | ||
</ul> | </ul> | ||
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</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/safety.html">Safety</a> |
</li> | </li> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a> | ||
| + | </li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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</header> | </header> | ||
| − | <section class="visual wow fadeInLeft | + | <section class="visual wow fadeInLeft animated" data-wow-duration="500ms" data-wow-delay="200ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 200ms; animation-name: fadeInLeft;"> |
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/1/13/T--worldshaper-XSHS--Home001.png" alt="" class="bg-stretch" style="width:100%"> |
</section> | </section> | ||
| − | <section id="about"> | + | <section id="about" style="background:rgba(242, 241, 241, 0.75)"> |
<div class="container"> | <div class="container"> | ||
| − | + | <div> | |
| − | + | ||
| − | + | <div class="row"> | |
| − | + | <div class="col-md-6 col-sm-6"> | |
| − | + | <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> | |
| − | + | <h2 class="v1" style="text-align: center;">Abstract</h2> | |
| − | + | ||
| − | + | <div style=" | |
| − | + | width: 100%; | |
| − | + | margin-left: 0; | |
| + | margin-top: -34px; | ||
| + | margin-bottom: -20px; | ||
| + | "> | ||
| + | <hr style=" | ||
| + | height: 3px; | ||
| + | background-color: #0c3d0c99!important; | ||
| + | width: 100%; | ||
| + | "> | ||
| + | <p style=" | ||
| + | margin-top: -18px; | ||
| + | font-size: 13px; | ||
| + | line-height: 20px; | ||
| + | font-family: ration cans; | ||
| + | " align="left"> </p> | ||
| + | </div> | ||
| + | <p class="itemstyle" style="margin: 0; text-indent:25px"> | ||
| + | There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it. | ||
| + | </p> | ||
| + | <p class="itemstyle" style="margin: 0; text-indent:25px"> | ||
| + | |||
| + | With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically. | ||
| + | </p> | ||
| + | <p class="itemstyle" style="margin: 0; text-indent:25px"> | ||
| + | Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms. | ||
| + | </p> | ||
| + | <!-- <p class="itemstyle" style="margin: 0"> | ||
| + | As we all know, smoking is extremely harmful to health, and secondhand | ||
| + | smoke is very easy to cause damage to public health. In daily life, people | ||
| + | have been suffering a lot. | ||
| + | </p> | ||
| + | <p class="itemstyle" style="margin: 0"> | ||
| + | Nicotine, which stems from ignited cigarettes, is so addictive that it is a | ||
| + | sort of aromatic poison to smokers. | ||
| + | </p> | ||
| + | <p class="itemstyle" style="margin: 0"> | ||
| + | Through literature reviewing, it is found that nicotine has adverse effects | ||
| + | on many tissues or organs of human body and is a major threat to | ||
| + | human health. | ||
| + | </p> | ||
| + | <p class="itemstyle" style="margin: 0"> | ||
| + | At the same time, the high content of nicotine in tobacco-leave waste | ||
| + | will pose a serious threat to the safety of groundwater in China. | ||
| + | </p> | ||
| + | <p class="itemstyle" style="margin: 0"> | ||
| + | Scholars at home and abroad have been paying attention to the | ||
| + | degradation of nicotine by microorganisms. Comparing with physical | ||
| + | and chemical methods, microbial degradation of nicotine is more | ||
| + | economical, more efficient and more sustainable. | ||
| + | </p>'' --> | ||
| + | <!-- <p class="itemstyle"> | ||
| + | Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water | ||
| + | pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating | ||
| + | and low-cost tool for public to monitor water quality around. | ||
| + | </p> --> | ||
| + | <!-- <p class="itemstyle"> | ||
| + | We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved | ||
| + | oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a | ||
| + | GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains | ||
| + | a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR | ||
| + | promoter was used to response to different concentrations of nitrate, nitrite and nitric, with | ||
| + | a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ | ||
| + | to sense the phosphate concentration and a RFP gene to report. | ||
| + | </p> --> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | <div class="col-md-6 col-sm-6"> | ||
| + | <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/9/9a/T--worldshaper-XSHS--Home002.png" alt=""> | ||
| + | </div> | ||
</div> | </div> | ||
| − | |||
</div> | </div> | ||
| − | + | <div class="row text-center"> | |
| − | <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;"> | + | <!-- <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> --> |
| − | + | <!-- <h2 class="v1">Abstract</h2> --> | |
| + | <!-- <div style=" | ||
| + | width: 28%; | ||
| + | margin: 0 auto; | ||
| + | margin-top: -34px; | ||
| + | "> | ||
| + | <hr style=" | ||
| + | height: 3px; | ||
| + | background-color: #0c3d0c99!important | ||
| + | "> | ||
| + | </div> --> | ||
| + | <!-- <p class="itemstyle"> | ||
| + | On this page, our team would like to share you with the fruits of our hard work during this significant summer. Here you can find the rough overview of our literature findings, the general purpose of our experiment and the rough results of our experiment. | ||
| + | </p> --> | ||
| + | <div class="" > | ||
| + | <!-- <p class="itemstyle" style="margin: 0"> | ||
| + | Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms. | ||
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| + | We have designed a portable blow-type biological detector based on Escherichia coli, which can be used to detect dissolved nicotine in water and approximate nicotine content in air. | ||
| + | </p> | ||
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| + | At the same time, we constructed several plasmids that could specifically respond to the presence of nicotine, and confirmed that two of them could work specifically. | ||
| + | </p> | ||
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| + | CONTAINING: | ||
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| + | The first generation of the plasmids: the nicotine induced NicA2 promoter + the reporter gene GFP; | ||
| + | </p> | ||
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| + | The second generation of the plasmids (The aim of constructing the second generation is to enhance the expression level of GFP): the nicotine induced NicA2 promoter + the reporter gene GFP; | ||
| + | </p> | ||
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| + | And a generation of failed plasmids: possible nicotine induced Nox promoter (which was later confirmed to have no specific expression of the presence of nicotine) + the reporter gene BFP and possible nicotine induced Nox promoter + the reporter gene RFP | ||
| + | </p> | ||
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| + | All in all, we are struggling to remove the secondhand smoke public and make sure there is no threat to public health as much as possible. | ||
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Latest revision as of 13:05, 17 October 2018
There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.
With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.
Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
Abstract
