Difference between revisions of "Team:Worldshaper-XSHS"

 
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<html>
 
<html>
 
<style type="text/css">
 
<style type="text/css">
 +
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     }
 
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     .colul a:hover{
 
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         margin-bottom: .5em;
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         font-family: arial !important;
−
         font-weight: 400 !important;
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         font-style: normal !important;
 
         font-style: normal !important;
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         line-height: 1.6em !important;
 
         line-height: 1.6em !important;
 
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         color: rgba(26,26,26,.7) !important;
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        font-size:18px!important;
 
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     .footer{
+
     p{
−
        background:rgba(0,0,0,1) !important;
+
      text-align:left!important;
 
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−
    #back-to-top{
+
 
−
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+
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−
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+
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+
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+
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−
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+
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        display:block;
+
−
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+
−
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+
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+
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+
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+
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         background:#d1d1d1;
+
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+
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+
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+
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+
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+
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+
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+
 
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 +
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 +
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 +
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 +
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 +
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 +
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</style>
 
</style>
 
     <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/wiki_edit.css">
 
     <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/wiki_edit.css">
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     <script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.fancybox.js"></script>
 
     <script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.fancybox.js"></script>
 
     <script src="http://igem2017.worldshaper.cn/XSHS/js/main.js"></script>
 
     <script src="http://igem2017.worldshaper.cn/XSHS/js/main.js"></script>
−
<script type="text/javascript">
 
−
    $(function () {
 
−
        //当点击跳转链接后，回到页面顶部位置
 
−
        $("#back-to-top").hide();
 
−
        window.onscroll = function () {
 
−
            var t = document.documentElement.scrollTop || document.body.scrollTop;  //获取距离页面顶部的距离
 
−
            var $x = $('#Ourteam').offset().top
 
−
            if (t >= $x - 80) {
 
−
                console.log(t, $x)
 
−
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−
                $("#back-to-top").fadeIn(1500);
 
−
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−
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−
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−
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−
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−
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−
            $('body,html').animate({ scrollTop: 0 }, 1000);
 
−
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−
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−
        $('.colul li').click(function () {
 
−
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−
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−
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−
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−
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−
  <p id="back-to-top">
 
−
    <a href="#top">
 
−
       
 
−
<img src="https://static.igem.org/mediawiki/2018/e/e5/T--worldshaper-XSHS--back.png">
 
−
To Top
 
−
    </a>
 
−
</p>
 
 
     <header id="top-bar" class="navbar-fixed-top animated-header  navbar navbar-default myheader"  >
 
     <header id="top-bar" class="navbar-fixed-top animated-header  navbar navbar-default myheader"  >
 
         <div class="container">
 
         <div class="container">
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                     <span class="icon-bar"></span>
 
                     <span class="icon-bar"></span>
 
                     <span class="icon-bar"></span>
 
                     <span class="icon-bar"></span>
−
                </button>
+
        </button>
 
                 <div class="navbar-brand " style="display:block;color:#fff;margin-left:15px;font-size:20px">
 
                 <div class="navbar-brand " style="display:block;color:#fff;margin-left:15px;font-size:20px">
 
                     <a href="https://2018.igem.org/Team:Worldshaper-XSHS">
 
                     <a href="https://2018.igem.org/Team:Worldshaper-XSHS">
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                     </a>
 
                     </a>
 
                 </div>
 
                 </div>
−
            </div>
+
      </div>
 
             <nav class="collapse navbar-collapse navbar-right" role="navigation">
 
             <nav class="collapse navbar-collapse navbar-right" role="navigation">
 
                 <div class="main-menu">
 
                 <div class="main-menu">
 
                     <ul class="nav navbar-nav navbar-right">
 
                     <ul class="nav navbar-nav navbar-right">
−
                         <li>
+
                          
−
                            <a href="https://2018.igem.org/Team:Worldshaper-XSHS">Home</a>
+
−
                        </li>
+
 
                         <li class="dropdown">
 
                         <li class="dropdown">
 
                             <a class="dropdown-toggle" data-toggle="dropdown">
 
                             <a class="dropdown-toggle" data-toggle="dropdown">
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                             <div class="dropdown-menu">
 
                             <div class="dropdown-menu">
 
                                 <ul>
 
                                 <ul>
 +
 
                                     <li>
 
                                     <li>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a>
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                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Team">Members</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Team">Members</a>
 
                                     </li>
 
                                     </li>
 +
                                   
 +
                                </ul>
 +
                            </div>
 +
                        </li>
 
                                     <li>
 
                                     <li>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Collaborations">Collaborations</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Collaborations">Collaborations</a>
 
                                     </li>
 
                                     </li>
−
                                </ul>
 
−
                            </div>
 
−
                        </li>
 
 
                         <li class="dropdown">
 
                         <li class="dropdown">
 
                             <a class="dropdown-toggle" data-toggle="dropdown">
 
                             <a class="dropdown-toggle" data-toggle="dropdown">
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                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a>
 
                                     </li>
 
                                     </li>
−
                                     <li>
+
                                      
−
                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a>
+
−
                                    </li>
+
 
                                     <li>
 
                                     <li>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a>
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                         <li class="dropdown">
 
                         <li class="dropdown">
 
                             <a class="dropdown-toggle" data-toggle="dropdown">
 
                             <a class="dropdown-toggle" data-toggle="dropdown">
−
                                 HUMAN PRACTICES
+
                                 HP
 
                                 <span class="caret"></span>
 
                                 <span class="caret"></span>
 
                             </a>
 
                             </a>
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                             <a href="https://2018.igem.org/Team:Worldshaper-XSHS/safety.html">Safety</a>
 
                             <a href="https://2018.igem.org/Team:Worldshaper-XSHS/safety.html">Safety</a>
 
                         </li>
 
                         </li>
 +
                                    <li>
 +
                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a>
 +
                                    </li>
 
                     </ul>
 
                     </ul>
 
                 </div>
 
                 </div>
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     </header>
 
     </header>
  
−
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+
     <section class="visual wow fadeInLeft animated" data-wow-duration="500ms" data-wow-delay="200ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 200ms; animation-name: fadeInLeft;">
−
        <strong  class="positiontitle">About Us</strong><!-- 这里是大图的标题 -->
+
         <img src="https://static.igem.org/mediawiki/2018/1/13/T--worldshaper-XSHS--Home001.png" alt="" class="bg-stretch" style="width:100%">  
−
         <img src="https://static.igem.org/mediawiki/2018/e/e6/T--worldshaper-XSHS--a001.png" alt="" class="bg-stretch" style="width:100%">
+
−
        <!-- 这里是大图的图片链接 -->
+
 
     </section>
 
     </section>
  
−
     <section class="pageInHere">
+
     <section id="about" style="background:rgba(242, 241, 241, 0.75)">
 
         <div class="container">
 
         <div class="container">
−
            <div class="row">
+
          <div>
−
                <div class="col-xs-2 xshs-box3  ">
+
             
−
                    <ul class="colul text-left">
+
                <div class="row">
−
                        <li class="colactive mt-1"><a href="#Ourteam">Our team</a></li>
+
                    <div class="col-md-6 col-sm-6">
−
                        <li class="mt-1"><a href="#OurSchool">Our School</a></li>
+
                        <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;">
−
                        <li class="mt-1"><a href="#Others">Others</a></li>
+
                            <h2 class="v1" style="text-align: center;">Abstract</h2>
−
                        <!-- 这里是页面定位锚点  href 对应代码的 “id属性  例如 id='xxx'  ”-->
+
                           
−
                    </ul>  
+
                            <div style="
−
                </div>
+
                                width: 100%;
−
                <div class="col-xs-10 ">
+
                                margin-left: 0;
−
                    <div class="xshs-box3">
+
                                margin-top: -34px;
−
                        <p class="v1" id="Ourteam">Our team</p>
+
                                margin-bottom: -20px;
−
                        <p class="itemstyle">
+
                            ">
−
                            Worldshaper XSHS, the name of the IGEM team of our Xiaoshan High School, translated as the " The New World's Shaper ", expresses
+
                            <hr style="
−
                            the new generation of young people's good expectations for future technology and the great ideals for contributing
+
                                height: 3px;
−
                            to mankind. Our team consists of 14 members and is guided by a number of teachers and doctors. Last year, the bio-detection
+
                                background-color: #0c3d0c99!important;
−
                            device for water oxygen content in our school's IGEM team won the first gold medal in the high school group in East
+
                                width: 100%;
−
                            China. This year, we will go all out to strive for further success!
+
                            ">
−
                        </p>
+
                                <p style="
 +
                                margin-top: -18px;
 +
                                font-size: 13px;
 +
                                line-height: 20px;
 +
                                font-family: ration cans;
 +
                            " align="left"> </p>
 +
                            </div>
 +
                            <p class="itemstyle" style="margin: 0; text-indent:25px">
 +
                                There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0; text-indent:25px">
 +
                               
 +
                                With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0; text-indent:25px">
 +
                                Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
 +
                            </p>
 +
                            <!-- <p class="itemstyle" style="margin: 0">
 +
                                As we all know, smoking is extremely harmful to health, and secondhand
 +
                                smoke is very easy to cause damage to public health. In daily life, people 
 +
                                have been suffering a lot.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                Nicotine, which stems from ignited cigarettes, is so addictive that it is a
 +
                                sort of aromatic poison to smokers.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                Through literature reviewing, it is found that nicotine has adverse effects
 +
                                on many tissues or organs of human body and is a major threat to
 +
                                human health.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                  At the same time, the high content of nicotine in tobacco-leave waste
 +
                                will pose a serious threat to the safety of groundwater in China.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                Scholars at home and abroad have been paying attention to the  
 +
                                degradation of nicotine by microorganisms. Comparing with physical
 +
                                and chemical methods, microbial degradation of nicotine is more
 +
                                economical, more efficient and more sustainable.
 +
                            </p>'' -->
 +
                            <!-- <p class="itemstyle">
 +
                                Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
 +
                                pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
 +
                                and low-cost tool for public to monitor water quality around.
 +
                            </p> -->
 +
                            <!-- <p class="itemstyle">
 +
                                We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
 +
                                oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a
 +
                                GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains
 +
                                a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR
 +
                                promoter was used to response to different concentrations of nitrate, nitrite and nitric, with
 +
                                a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
 +
                                to sense the phosphate concentration and a RFP gene to report.
 +
                            </p> -->
 +
                        </div>
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                     </div>
 
                     </div>
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                     <div class="xshs-box2">
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                     <div class="col-md-6 col-sm-6">
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                         <p>
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                         <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;">
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                            <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/e/e6/T--worldshaper-XSHS--a001.png" />
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                             <img src="https://static.igem.org/mediawiki/2018/9/9a/T--worldshaper-XSHS--Home002.png" alt="">
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                        </p>
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                         </div>
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                        <p>
+
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                             <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/0/07/T--worldshaper-XSHS--a002.png" />
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                         </p>
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                     </div>
 
                     </div>
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                     <div class="xshs-box3">
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                </div>
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                         <p class="v1" id="OurSchool">Our School</p>
+
<div class="row text-center">
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                         <p class="itemstyle">
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                     <!-- <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> -->
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                             We are from Xiaoshan High School, Zhejiang Province, a school founded in 1938 with a long history in Hangzhou, China. The
+
                         <!-- <h2 class="v1">Abstract</h2> -->
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                            school adheres to the motto: "seek the truth, seek the innovation, seek the beauty and seeking reality". We have trained
+
                        <!-- <div style="
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                            large quantities of outstanding students over the years. This is our school:
+
                            width: 28%;
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                        </p>
+
                            margin: 0 auto;
 +
                            margin-top: -34px;
 +
                        ">
 +
                        <hr style="
 +
                            height: 3px;
 +
                            background-color: #0c3d0c99!important
 +
                        ">
 +
                        </div> -->
 +
                         <!-- <p class="itemstyle">
 +
                             On this page, our team would like to share you with the fruits of our hard work during this significant summer. Here you can find the rough overview of our literature findings, the general purpose of our experiment and the rough results of our experiment.
 +
                        </p> -->
 +
                        <div class="" >
 +
                            <!-- <p class="itemstyle" style="margin: 0">
 +
                                Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
 +
                            </p> -->
 +
                            <!-- <p class="itemstyle" style="margin: 0">
 +
                                  We have designed a portable blow-type biological detector based on Escherichia coli, which can be used to detect dissolved nicotine in water and approximate nicotine content in air.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                At the same time, we constructed several plasmids that could specifically respond to the presence of nicotine, and confirmed that two of them could work specifically.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                CONTAINING:
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                The first generation of the plasmids: the nicotine induced NicA2 promoter + the reporter gene GFP;
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                The second generation of the plasmids (The aim of constructing the second generation is to enhance the expression level of GFP): the nicotine induced NicA2 promoter + the reporter gene GFP;
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                And a generation of failed plasmids: possible nicotine induced Nox promoter (which was later confirmed to have no specific expression of the presence of nicotine) + the reporter gene BFP and possible nicotine induced Nox promoter + the reporter gene RFP
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                All in all, we are struggling to remove the secondhand smoke public and make sure there is no threat to public health as much as possible.
 +
                            </p> -->
 +
                        </div> 
 +
                     
 
                     </div>
 
                     </div>
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                     <div class="xshs-box2">
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                     <div>
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                         <p>
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                         <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;">
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                            <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/c/c0/T--worldshaper-XSHS--a003.png" style="width: 133%;height: 150%"
+
                             <img src="https://static.igem.org/mediawiki/2018/e/e9/T--worldshaper-XSHS--xshs001.png" alt="">
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                            />
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                         </div>
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                        </p>
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                        <p>
+
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                             <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/6/61/T--worldshaper-XSHS--a004.png" />
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                        </p>
+
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                        <p>
+
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                            <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/6/67/T--worldshaper-XSHS--a005.png" />
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                         </p>
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                     </div>
 
                     </div>
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                    <div class="xshs-box3">
 
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                        <p class="v1" id="Others">Others</p>
 
−
                        <p class="itemstyle">
 
−
                            Logo: We have designed our own team logo and flag. This is our logo:
 
−
                        </p>
 
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                    </div>
 
−
                    <div class="xshs-box2">
 
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                        <p>
 
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                            <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/7/78/T--worldshaper-XSHS--a006.png" />
 
−
                        </p>
 
−
                    </div>
 
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                    <div class="xshs-box3">
 
−
                        <p>Logo meaning:</p>
 
−
                        <p style="color: red!important">
 
−
                            这里输入logo意义
 
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                        </p>
 
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                    </div>
 
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                    <div class="xshs-box3">
 
−
                        <p class="itemstyle">
 
−
                            Whenever we go out to participate in activities, we all wear our uniform. We all love our uniform:
 
−
                        </p>
 
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                    </div>
 
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                    <div class="xshs-box2">
 
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                        <p>
 
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                            <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/6/67/T--worldshaper-XSHS--a007.png" />
 
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                        </p>
 
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                    </div>
 
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                    <div class="xshs-box3">
 
−
                        <p class="itemstyle">
 
−
                            In addition, the money to buy the uniforms and the flag is all from fund-raising. Thanks to those who support us
 
−
                        </p>
 
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                    <div class="xshs-box2">
 
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                        <p>
 
−
                            <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/e/e1/T--worldshaper-XSHS--a008.png" />
 
−
                        </p>
 
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                    </div>
 
−
 
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
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        </div>
 
 
     </section>
 
     </section>
  
−
     <footer class="footer">
+
     <footer class="footer">
 
         <div class="container">
 
         <div class="container">
 
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Latest revision as of 13:05, 17 October 2018

Worldshaper- XSHS

Abstract

There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.

With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.

Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.

  • Worldshaper-XSHS, Xiaoshan High School

    Adress: No.538,Gongxiu Road,Xiaoshan District,Hangzhou,Zhejiang Province,China