Difference between revisions of "Team:Worldshaper-XSHS"

 
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                            <a href="https://2018.igem.org/Team:Worldshaper-XSHS">Home</a>
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                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a>
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                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Team">Members</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Team">Members</a>
 
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                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Collaborations">Collaborations</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Collaborations">Collaborations</a>
 
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                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a>
 
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a>
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                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a>
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                                 HUMAN PRACTICES
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                             <a href="https://2018.igem.org/Team:Worldshaper-XSHS/safety.html">Safety</a>
 
                             <a href="https://2018.igem.org/Team:Worldshaper-XSHS/safety.html">Safety</a>
 
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a>
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                        <h2 class="v1">Abstract</h2>
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                            <h2 class="v1" style="text-align: center;">Abstract</h2>
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                            On this page, our team would like to share you with the fruits of our hard work during this significant summer. Here you can find the rough overview of our literature findings, the general purpose of our experiment and the rough results of our experiment.
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                                  It's better to quit smoking.
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                            " align="left"> </p>
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                                There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.
 
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                                 China is not only the world's largest tobacco producer, but also the  
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                                world's largest tobacco consumer.
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                                With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.
 
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                                Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
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                            </p>
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                                 As we all know, smoking is extremely harmful to health, and secondhand  
 
                                 As we all know, smoking is extremely harmful to health, and secondhand  
 
                                 smoke is very easy to cause damage to public health. In daily life, people   
 
                                 smoke is very easy to cause damage to public health. In daily life, people   
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                                 and chemical methods, microbial degradation of nicotine is more  
 
                                 and chemical methods, microbial degradation of nicotine is more  
 
                                 economical, more efficient and more sustainable.
 
                                 economical, more efficient and more sustainable.
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                            " align="left">We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. </p>
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                                 Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
 
                                 Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
 
                                 pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
 
                                 pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
 
                                 and low-cost tool for public to monitor water quality around.
 
                                 and low-cost tool for public to monitor water quality around.
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                                 We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
 
                                 We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
 
                                 oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a
 
                                 oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a
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                                 a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
 
                                 a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
 
                                 to sense the phosphate concentration and a RFP gene to report.
 
                                 to sense the phosphate concentration and a RFP gene to report.
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                        <!-- <p class="itemstyle">
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                            On this page, our team would like to share you with the fruits of our hard work during this significant summer. Here you can find the rough overview of our literature findings, the general purpose of our experiment and the rough results of our experiment.
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                                Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
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                                  We have designed a portable blow-type biological detector based on Escherichia coli, which can be used to detect dissolved nicotine in water and approximate nicotine content in air.
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                            </p>
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                            <p class="itemstyle" style="margin: 0">
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                                At the same time, we constructed several plasmids that could specifically respond to the presence of nicotine, and confirmed that two of them could work specifically.
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                                CONTAINING:
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                                The first generation of the plasmids: the nicotine induced NicA2 promoter + the reporter gene GFP;
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                                The second generation of the plasmids (The aim of constructing the second generation is to enhance the expression level of GFP): the nicotine induced NicA2 promoter + the reporter gene GFP;
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                            </p>
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                                And a generation of failed plasmids: possible nicotine induced Nox promoter (which was later confirmed to have no specific expression of the presence of nicotine) + the reporter gene BFP and possible nicotine induced Nox promoter + the reporter gene RFP
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                                All in all, we are struggling to remove the secondhand smoke public and make sure there is no threat to public health as much as possible.
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Latest revision as of 13:05, 17 October 2018

Worldshaper- XSHS

Abstract

There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.

With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.

Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.

  • Worldshaper-XSHS, Xiaoshan High School

    Adress: No.538,Gongxiu Road,Xiaoshan District,Hangzhou,Zhejiang Province,China