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<a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a> | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a> | ||
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<a href="https://2018.igem.org/Team:Worldshaper-XSHS/Collaborations">Collaborations</a> | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Collaborations">Collaborations</a> | ||
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<a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a> | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a> | ||
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<a href="https://2018.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a> | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a> | ||
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+ | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a> | ||
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− | + | There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it. | |
</p> | </p> | ||
− | <p class="itemstyle" style="margin: 0"> | + | <p class="itemstyle" style="margin: 0; text-indent:25px"> |
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− | + | With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically. | |
</p> | </p> | ||
− | <p class="itemstyle" style="margin: 0"> | + | <p class="itemstyle" style="margin: 0; text-indent:25px"> |
+ | Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms. | ||
+ | </p> | ||
+ | <!-- <p class="itemstyle" style="margin: 0"> | ||
As we all know, smoking is extremely harmful to health, and secondhand | As we all know, smoking is extremely harmful to health, and secondhand | ||
smoke is very easy to cause damage to public health. In daily life, people | smoke is very easy to cause damage to public health. In daily life, people | ||
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and chemical methods, microbial degradation of nicotine is more | and chemical methods, microbial degradation of nicotine is more | ||
economical, more efficient and more sustainable. | economical, more efficient and more sustainable. | ||
− | </p>'' | + | </p>'' --> |
<!-- <p class="itemstyle"> | <!-- <p class="itemstyle"> | ||
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water | Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water | ||
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− | <h2 class="v1">Abstract</h2> | + | <!-- <h2 class="v1">Abstract</h2> --> |
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"> | "> | ||
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<!-- <p class="itemstyle"> | <!-- <p class="itemstyle"> | ||
On this page, our team would like to share you with the fruits of our hard work during this significant summer. Here you can find the rough overview of our literature findings, the general purpose of our experiment and the rough results of our experiment. | On this page, our team would like to share you with the fruits of our hard work during this significant summer. Here you can find the rough overview of our literature findings, the general purpose of our experiment and the rough results of our experiment. | ||
</p> --> | </p> --> | ||
<div class="" > | <div class="" > | ||
− | <p class="itemstyle" style="margin: 0"> | + | <!-- <p class="itemstyle" style="margin: 0"> |
− | + | Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms. | |
+ | </p> --> | ||
+ | <!-- <p class="itemstyle" style="margin: 0"> | ||
+ | We have designed a portable blow-type biological detector based on Escherichia coli, which can be used to detect dissolved nicotine in water and approximate nicotine content in air. | ||
</p> | </p> | ||
<p class="itemstyle" style="margin: 0"> | <p class="itemstyle" style="margin: 0"> | ||
− | + | At the same time, we constructed several plasmids that could specifically respond to the presence of nicotine, and confirmed that two of them could work specifically. | |
− | + | ||
</p> | </p> | ||
<p class="itemstyle" style="margin: 0"> | <p class="itemstyle" style="margin: 0"> | ||
− | + | CONTAINING: | |
− | + | ||
− | + | ||
</p> | </p> | ||
<p class="itemstyle" style="margin: 0"> | <p class="itemstyle" style="margin: 0"> | ||
− | + | The first generation of the plasmids: the nicotine induced NicA2 promoter + the reporter gene GFP; | |
− | + | ||
</p> | </p> | ||
<p class="itemstyle" style="margin: 0"> | <p class="itemstyle" style="margin: 0"> | ||
− | + | The second generation of the plasmids (The aim of constructing the second generation is to enhance the expression level of GFP): the nicotine induced NicA2 promoter + the reporter gene GFP; | |
− | + | ||
− | + | ||
</p> | </p> | ||
<p class="itemstyle" style="margin: 0"> | <p class="itemstyle" style="margin: 0"> | ||
− | + | And a generation of failed plasmids: possible nicotine induced Nox promoter (which was later confirmed to have no specific expression of the presence of nicotine) + the reporter gene BFP and possible nicotine induced Nox promoter + the reporter gene RFP | |
− | + | ||
</p> | </p> | ||
<p class="itemstyle" style="margin: 0"> | <p class="itemstyle" style="margin: 0"> | ||
− | + | All in all, we are struggling to remove the secondhand smoke public and make sure there is no threat to public health as much as possible. | |
− | + | </p> --> | |
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− | + | ||
− | </p> | + | |
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− | <img src="https://static.igem.org/mediawiki/ | + | <img src="https://static.igem.org/mediawiki/2018/e/e9/T--worldshaper-XSHS--xshs001.png" alt=""> |
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− | <img src="https://static.igem.org/mediawiki/ | + | <img src="https://static.igem.org/mediawiki/2018/9/96/T--worldshaper-XSHS--wechat.png" alt=""> |
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</ul> | </ul> |
Latest revision as of 13:05, 17 October 2018
There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.
With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.
Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
Abstract