Difference between revisions of "Team:Worldshaper-XSHS"

 
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                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a>
 
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                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a>
 
                                         <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a>
 
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                                 HUMAN PRACTICES
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                             <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Applied_Design">Applied Design</a>
 
                             <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Applied_Design">Applied Design</a>
 
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a>
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                             <h2 class="v1" style="text-align: center;">Abstract</h2>
 
                             <h2 class="v1" style="text-align: center;">Abstract</h2>
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                                There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.
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                                There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.
 
                             </p>
 
                             </p>
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                                 With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.
 
                                 With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.
 
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                                Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
 +
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                            <!-- <p class="itemstyle" style="margin: 0">
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                                As we all know, smoking is extremely harmful to health, and secondhand
 +
                                smoke is very easy to cause damage to public health. In daily life, people  
 +
                                have been suffering a lot.
 +
                            </p>
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                                 Nicotine, which stems from ignited cigarettes, is so addictive that it is a  
 
                                 Nicotine, which stems from ignited cigarettes, is so addictive that it is a  
 
                                 sort of aromatic poison to smokers.
 
                                 sort of aromatic poison to smokers.
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                         <!-- <p class="itemstyle">
 
                         <!-- <p class="itemstyle">
 
                             On this page, our team would like to share you with the fruits of our hard work during this significant summer. Here you can find the rough overview of our literature findings, the general purpose of our experiment and the rough results of our experiment.
 
                             On this page, our team would like to share you with the fruits of our hard work during this significant summer. Here you can find the rough overview of our literature findings, the general purpose of our experiment and the rough results of our experiment.
 
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                                  Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
+
                                Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
−
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                             <!-- <p class="itemstyle" style="margin: 0">
 
                                   We have designed a portable blow-type biological detector based on Escherichia coli, which can be used to detect dissolved nicotine in water and approximate nicotine content in air.
 
                                   We have designed a portable blow-type biological detector based on Escherichia coli, which can be used to detect dissolved nicotine in water and approximate nicotine content in air.
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Latest revision as of 13:05, 17 October 2018

Worldshaper- XSHS

Abstract

There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.

With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.

Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.

  • Worldshaper-XSHS, Xiaoshan High School

    Adress: No.538,Gongxiu Road,Xiaoshan District,Hangzhou,Zhejiang Province,China