Difference between revisions of "Team:Worldshaper-XSHS"

 
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{{Worldshaper-XSHS}}
 
 
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                    <a href="https://2018.igem.org/Team:Worldshaper-XSHS">
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                        Worldshaper- <b>XSHS</b>
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            <nav class="collapse navbar-collapse navbar-right" role="navigation">
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                                Team
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                                <span class="caret"></span>
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                            </a>
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                            <div class="dropdown-menu">
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                                <ul>
  
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a>
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                                    </li>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Team">Members</a>
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                                    </li>
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                                </ul>
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                        </li>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Collaborations">Collaborations</a>
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                                    </li>
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                        <li class="dropdown">
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                            <a class="dropdown-toggle" data-toggle="dropdown">
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                                Project
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                                <span class="caret"></span>
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                            </a>
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                            <div class="dropdown-menu">
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                                <ul>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Background.html">Background</a>
 +
                                    </li>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/design.html">Design</a>
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                                    </li>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Protocols-methods.html">Protocols and methods</a>
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                                    </li>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/result.html">Results</a>
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                                    </li>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a>
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                                    </li>
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 +
                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a>
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                                    </li>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Model">Model</a>
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                                    </li>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Demonstrate">Demonstrate</a>
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                                    </li>
  
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                                </ul>
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                            </div>
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                        </li>
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                        <li>
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                            <a href="https://2018.igem.org/Team:Worldshaper-XSHS/parts.html">Parts</a>
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                        </li>
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                        <li class="dropdown">
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                            <a class="dropdown-toggle" data-toggle="dropdown">
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                                HP
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                                <span class="caret"></span>
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                            </a>
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                            <div class="dropdown-menu">
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                                <ul>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/HP/Silver">Silver</a>
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                                    </li>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/HP/Gold_Integrated">Integrated and Gold</a>
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                                    </li>
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                                    <li>
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                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Engagement">Engagement</a>
 +
                                    </li>
 +
                                </ul>
 +
                            </div>
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                        </li>
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                        <li>
 +
                            <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Applied_Design">Applied Design</a>
 +
                        </li>
 +
                        <li class="dropdown">
 +
                            <a class="dropdown-toggle" data-toggle="dropdown">
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                                Achievement
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                                <span class="caret"></span>
 +
                            </a>
 +
                            <div class="dropdown-menu">
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                                <ul>
 +
                                    <li>
 +
                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Medal-requirements.html">Medal requirements</a>
 +
                                    </li>
 +
                                </ul>
 +
                            </div>
 +
                        </li>
 +
                        <li>
 +
                            <a href="https://2018.igem.org/Team:Worldshaper-XSHS/safety.html">Safety</a>
 +
                        </li>
 +
                                    <li>
 +
                                        <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a>
 +
                                    </li>
 +
                    </ul>
 +
                </div>
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            </nav>
 +
        </div>
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    </header>
  
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<h1> Welcome to IGEM 2018! </h1>
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        <img src="https://static.igem.org/mediawiki/2018/1/13/T--worldshaper-XSHS--Home001.png" alt="" class="bg-stretch" style="width:100%">  
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h3> Uploading pictures and files </h3>
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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UPLOAD FILES
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<h3> Wiki template information </h3>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h3> Editing your wiki </h3>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<a href="https://2018.igem.org/wiki/index.php?title=Team:Worldshaper-XSHS&action=edit">
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EDIT PAGE
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
 
+
  
 +
    <section id="about" style="background:rgba(242, 241, 241, 0.75)">
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        <div class="container">
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          <div>
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                <div class="row">
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                    <div class="col-md-6 col-sm-6">
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                        <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;">
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                            <h2 class="v1" style="text-align: center;">Abstract</h2>
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                            <div style="
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                                width: 100%;
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                                width: 100%;
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                            ">
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                                <p style="
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                                margin-top: -18px;
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                                font-size: 13px;
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                                font-family: ration cans;
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                            " align="left"> </p>
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                            </div>
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                            <p class="itemstyle" style="margin: 0; text-indent:25px">
 +
                                There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0; text-indent:25px">
 +
                               
 +
                                With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0; text-indent:25px">
 +
                                Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
 +
                            </p>
 +
                            <!-- <p class="itemstyle" style="margin: 0">
 +
                                As we all know, smoking is extremely harmful to health, and secondhand
 +
                                smoke is very easy to cause damage to public health. In daily life, people 
 +
                                have been suffering a lot.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                Nicotine, which stems from ignited cigarettes, is so addictive that it is a
 +
                                sort of aromatic poison to smokers.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                Through literature reviewing, it is found that nicotine has adverse effects
 +
                                on many tissues or organs of human body and is a major threat to
 +
                                human health.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                  At the same time, the high content of nicotine in tobacco-leave waste
 +
                                will pose a serious threat to the safety of groundwater in China.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                Scholars at home and abroad have been paying attention to the
 +
                                degradation of nicotine by microorganisms. Comparing with physical
 +
                                and chemical methods, microbial degradation of nicotine is more
 +
                                economical, more efficient and more sustainable.
 +
                            </p>'' -->
 +
                            <!-- <p class="itemstyle">
 +
                                Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water
 +
                                pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating
 +
                                and low-cost tool for public to monitor water quality around.
 +
                            </p> -->
 +
                            <!-- <p class="itemstyle">
 +
                                We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved
 +
                                oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a
 +
                                GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains
 +
                                a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR
 +
                                promoter was used to response to different concentrations of nitrate, nitrite and nitric, with
 +
                                a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’
 +
                                to sense the phosphate concentration and a RFP gene to report.
 +
                            </p> -->
 +
                        </div>
 +
   
 +
                    </div>
 +
                    <div class="col-md-6 col-sm-6">
 +
                        <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/9/9a/T--worldshaper-XSHS--Home002.png" alt="">
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
<div class="row text-center">
 +
                    <!-- <div class="block wow fadeInLeft animated" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInLeft;"> -->
 +
                        <!-- <h2 class="v1">Abstract</h2> -->
 +
                        <!-- <div style="
 +
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 +
                            margin: 0 auto;
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                            margin-top: -34px;
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                        ">
 +
                        <hr style="
 +
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                            background-color: #0c3d0c99!important
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                        ">
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                        </div> -->
 +
                        <!-- <p class="itemstyle">
 +
                            On this page, our team would like to share you with the fruits of our hard work during this significant summer. Here you can find the rough overview of our literature findings, the general purpose of our experiment and the rough results of our experiment.
 +
                        </p> -->
 +
                        <div class="" >
 +
                            <!-- <p class="itemstyle" style="margin: 0">
 +
                                Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.
 +
                            </p> -->
 +
                            <!-- <p class="itemstyle" style="margin: 0">
 +
                                  We have designed a portable blow-type biological detector based on Escherichia coli, which can be used to detect dissolved nicotine in water and approximate nicotine content in air.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                At the same time, we constructed several plasmids that could specifically respond to the presence of nicotine, and confirmed that two of them could work specifically.
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                CONTAINING:
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                The first generation of the plasmids: the nicotine induced NicA2 promoter + the reporter gene GFP;
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                The second generation of the plasmids (The aim of constructing the second generation is to enhance the expression level of GFP): the nicotine induced NicA2 promoter + the reporter gene GFP;
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                And a generation of failed plasmids: possible nicotine induced Nox promoter (which was later confirmed to have no specific expression of the presence of nicotine) + the reporter gene BFP and possible nicotine induced Nox promoter + the reporter gene RFP
 +
                            </p>
 +
                            <p class="itemstyle" style="margin: 0">
 +
                                All in all, we are struggling to remove the secondhand smoke public and make sure there is no threat to public health as much as possible.
 +
                            </p> -->
 +
                        </div> 
 +
                     
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                    </div>
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                    <div>
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                        <div class="block wow fadeInRight" data-wow-delay=".3s" data-wow-duration="500ms" style="visibility: hidden; animation-duration: 500ms; animation-delay: 0.3s; animation-name: none;">
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                            <img src="https://static.igem.org/mediawiki/2018/e/e9/T--worldshaper-XSHS--xshs001.png" alt="">
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
            </div>
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    </section>
  
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                        <p>Worldshaper-XSHS, Xiaoshan High School</p>
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 +
                            Adress: No.538,Gongxiu Road,Xiaoshan District,Hangzhou,Zhejiang Province,China
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Latest revision as of 13:05, 17 October 2018

Worldshaper- XSHS

Abstract

There is a great number of smokers in China. Although relative regulations have been issued, the number still keeps increasing. Smoking not only ruins the health of the smokers, but also does harm to the the people around by releasing toxic materials like nicotine, which is a big threat to human beings. Therefore, we are supposed to design an easy-to-use, low-cost biological detection tool for the public to measure the nicotine content and thus reduce the damage caused by it.

With the knowledge of synthetic biology, we have constructed multiple plasmids that can specifically respond to the presence of nicotine, including: first-version plasmids: NicA2 promoter of nicotine-induced expression + reporting component GFP. To make the expression of GFP clearer, second-version plasmids were constructed(The target of constructing the second version is to enhance the expression level of GFP): NicA2 promoter + T7 RNA polymerase gene + T7 promoter + GFP. It has been confirmed that two versions of components can be expressed specifically.

Based on this kind of technical support, we designed a prototype bio-detector which can be used to detect the nicotine dissolved in water, and we plan to put it into practice by using an alarm device to monitor excessive nicotine. Hopefully, the alarm device could be used in public smoking rooms.

  • Worldshaper-XSHS, Xiaoshan High School

    Adress: No.538,Gongxiu Road,Xiaoshan District,Hangzhou,Zhejiang Province,China