Difference between revisions of "Team:Worldshaper-XSHS/Demonstrate"
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Revision as of 16:19, 17 October 2018
Experiment demonstration
We built Several parts （https://2018.igem.org/Team:Worldshaper-XSHS/parts.html）in the project, including parts related to nicotine detection (BBa_K2827005, BBa_K2827006). We tested and compared the two-generation detection system, making sure that it could be used as the nicotine concentration detectionaa （https://2018.igem.org/Team:Worldshaper-XSHS/result.html）. Based on its finding, we designed the detection device model.
Product demonstration
According to the results of the survey and interview, we designed two generations of products. Due to limited time, we only made models of the first generation product. The design of the second generation model has been improved based on the first generation, which makes up for the deficiency of it.
First generation product
（wiki：https://2018.igem.org/Team:Worldshaper-XSHS）
The first generation product was a stationary nicotine-testing device (see wiki). The instrument is divided into three parts: air inlet pipe, box body, and air outlet pipe. The chamber is filled with bacteria liquid. A fan is used to extract the upper part of the gas to form a low-pressure area. The atmospheric pressure will pump the gas to be tested from the other side into the inlet pipe, which is bent down and connected to the bottom of the device, so that the gas can fully contact with the liquid and water-soluble nicotine can be transferred from the air into the bacteria liquid. After that, we got the bacterial fluid that expressed the fluorescent protein under the effect of nicotine, measured the fluorescence, and calculated the nicotine concentration. We used 3D printing technology to construct our product design into a physical model. The main body of the product model is a cuboid of 15cm in length, 7cm in width and 7cm in height (Figure1, Figure2, Figure3). The air inlet and outlet with diameter of 2cm are also provided. When we did the simulation experiment, we found that the existing device had the disadvantages such as imprecise measurement, poor safety, and insensitivity, so we made further improvement.
Second generation product
In the design of the second generation product, we reduced the device size (Figure4, Figure5) the actual internal bottom area was about 16cm squared-and the amount of bacterial liquid needed to work(Figure6, Figure7). The equipment also adopts the pump type piston pumping way, which enable filters device to reduce the possible escherichia coli leakage properly. Because the piston is used to pump air, the gas is a fixed column of air volume when the piston pumps back and forth, bringing great convenience for us to exactly extract the volume we want.
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