Difference between revisions of "Team:Worldshaper-XSHS/result.html"
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| − | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/wiki_edit.css"> | + | url('https://static.igem.org/mediawiki/2018/4/44/T--worldshaper-XSHS--semibold.otf'); |
| − | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/bootstrap.min.css"> | + | } |
| − | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/ionicons.min.css"> | + | </style><!-- style --> |
| − | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/animate.css"> | + | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/wiki_edit.css"> |
| − | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/slider.css"> | + | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/bootstrap.min.css"> |
| − | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/owl.carousel.css"> | + | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/ionicons.min.css"> |
| − | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/owl.theme.css"> | + | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/animate.css"> |
| − | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/jquery.fancybox.css"> | + | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/slider.css"> |
| − | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/main.css"> | + | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/owl.carousel.css"> |
| − | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/responsive.css"> | + | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/owl.theme.css"> |
| − | <script src="http://igem2017.worldshaper.cn/XSHS/js/vendor/modernizr-2.6.2.min.js"></script> | + | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/jquery.fancybox.css"> |
| − | <script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.min.js"></script> | + | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/main.css"> |
| − | <script src="http://igem2017.worldshaper.cn/XSHS/js/owl.carousel.min.js"></script> | + | <link rel="stylesheet" type="text/css" href="http://igem2017.worldshaper.cn/XSHS/css/responsive.css"> |
| − | <script src="http://igem2017.worldshaper.cn/XSHS/js/bootstrap.min.js"></script> | + | <script src="http://igem2017.worldshaper.cn/XSHS/js/vendor/modernizr-2.6.2.min.js"></script> |
| − | <script src="http://igem2017.worldshaper.cn/XSHS/js/wow.min.js"></script> | + | <script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.min.js"></script> |
| − | <script src="http://igem2017.worldshaper.cn/XSHS/js/slider.js"></script> | + | <script src="http://igem2017.worldshaper.cn/XSHS/js/owl.carousel.min.js"></script> |
| − | <script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.fancybox.js"></script> | + | <script src="http://igem2017.worldshaper.cn/XSHS/js/bootstrap.min.js"></script> |
| − | <script src="http://igem2017.worldshaper.cn/XSHS/js/main.js"></script> | + | <script src="http://igem2017.worldshaper.cn/XSHS/js/wow.min.js"></script> |
| + | <script src="http://igem2017.worldshaper.cn/XSHS/js/slider.js"></script> | ||
| + | <script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.fancybox.js"></script> | ||
| + | <script src="http://igem2017.worldshaper.cn/XSHS/js/main.js"></script> | ||
<script type="text/javascript"> | <script type="text/javascript"> | ||
$(function () { | $(function () { | ||
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window.onscroll = function () { | window.onscroll = function () { | ||
var t = document.documentElement.scrollTop || document.body.scrollTop; //获取距离页面顶部的距离 | var t = document.documentElement.scrollTop || document.body.scrollTop; //获取距离页面顶部的距离 | ||
| − | var $x = $('# | + | var $x = $('#Ourteam').offset().top |
if (t >= $x - 80) { | if (t >= $x - 80) { | ||
console.log(t, $x) | console.log(t, $x) | ||
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}) | }) | ||
}) | }) | ||
| − | </script> | + | </script><!-- css and js --> |
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| − | + | <div class="container"> | |
| − | + | <div class="navbar-header" > | |
| − | + | <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-collapse"> | |
| − | + | <span class="sr-only">Toggle navigation</span> | |
| − | + | <span class="icon-bar"></span> | |
| − | + | <span class="icon-bar"></span> | |
| − | + | <span class="icon-bar"></span> | |
| − | + | </button> | |
| − | + | <div class="navbar-brand " style="display:block;color:#fff;margin-left:15px;font-size:20px"> | |
| − | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS"> | |
| − | + | Worldshaper- <b>XSHS</b> | |
| − | + | </a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <nav class="collapse navbar-collapse navbar-right" role="navigation"> | |
| − | + | <div class="main-menu"> | |
| − | + | <ul class="nav navbar-nav navbar-right"> | |
| − | + | ||
| − | + | <li class="dropdown"> | |
| − | + | <a class="dropdown-toggle" data-toggle="dropdown"> | |
| − | + | Team | |
| − | + | <span class="caret"></span> | |
| − | + | </a> | |
| − | + | <div class="dropdown-menu"> | |
| − | + | <ul> | |
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| − | < | + | <li> |
| − | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a> | |
| − | + | </li> | |
| − | + | <li> | |
| − | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Team">Members</a> | |
| − | + | </li> | |
| − | + | ||
| − | + | </ul> | |
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| − | </ | + | |
</div> | </div> | ||
| − | + | </li> | |
| − | + | <li> | |
| − | < | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Collaborations">Collaborations</a> |
| − | + | </li> | |
| − | + | <li class="dropdown"> | |
| − | + | <a class="dropdown-toggle" data-toggle="dropdown"> | |
| − | + | Project | |
| − | + | <span class="caret"></span> | |
| − | + | </a> | |
| + | <div class="dropdown-menu"> | ||
| + | <ul> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Background.html">Background</a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/design.html">Design</a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Protocols-methods.html">Protocols and methods</a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/result.html">Results</a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a> | ||
| + | </li> | ||
| + | |||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Model">Model</a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Demonstrate">Demonstrate</a> | ||
| + | </li> | ||
| − | + | </ul> | |
| − | + | ||
| − | + | ||
| − | < | + | |
</div> | </div> | ||
| − | + | </li> | |
| − | + | <li> | |
| − | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/parts.html">Parts</a> | |
| − | + | </li> | |
| − | + | <li class="dropdown"> | |
| − | + | <a class="dropdown-toggle" data-toggle="dropdown"> | |
| − | </ | + | HP |
| − | + | <span class="caret"></span> | |
| − | + | </a> | |
| − | + | <div class="dropdown-menu"> | |
| − | + | <ul> | |
| − | <div class=" | + | <li> |
| − | < | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/HP/Silver">Silver</a> |
| + | </li> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/HP/Gold_Integrated">Integrated and Gold</a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Engagement">Engagement</a> | ||
| + | </li> | ||
| + | </ul> | ||
</div> | </div> | ||
| − | + | </li> | |
| − | + | <li> | |
| − | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Applied_Design">Applied Design</a> | |
| − | + | </li> | |
| − | + | <li class="dropdown"> | |
| − | + | <a class="dropdown-toggle" data-toggle="dropdown"> | |
| − | </ | + | Achievement |
| − | + | <span class="caret"></span> | |
| − | + | </a> | |
| − | + | <div class="dropdown-menu"> | |
| − | + | <ul> | |
| − | <div class=" | + | <li> |
| − | < | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Medal-requirements.html">Medal requirements</a> |
| + | </li> | ||
| + | </ul> | ||
</div> | </div> | ||
| − | + | </li> | |
| − | + | <li> | |
| − | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/safety.html">Safety</a> | |
| − | + | </li> | |
| − | + | <li> | |
| − | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a> | |
| − | + | </li> | |
| − | + | </ul> | |
| − | + | ||
| − | + | ||
| − | </ | + | |
</div> | </div> | ||
| − | + | </nav> | |
| − | + | </div> | |
| − | + | </header><!-- header --> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | <section class="visual wow fadeInLeft text-center animated" data-wow-duration="500ms" data-wow-delay="200ms" style="visibility: visible; animation-duration: 500ms; animation-delay: 200ms; animation-name: fadeInLeft;"> | |
| − | + | <strong class="positiontitle">Results</strong><!-- 这里是大图的标题 --> | |
| − | + | <img src="https://static.igem.org/mediawiki/2018/c/c3/T--Worldshaper-XSHS--s004.jpg | |
| − | + | " alt="" class="bg-stretch" style="width:100%"> | |
| − | + | <!-- 这里是大图的图片链接 --> | |
| − | + | </section> | |
| − | + | <section class="pageInHere"> | |
| − | + | <div class="container"> | |
| − | < | + | <div class="row"> |
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | |
| − | </ | + | <div class="col-xs-2 xshs-box3 "> |
| − | + | <ul class="colul text-left"style="width: 180px;font-size: 15px!important"> | |
| − | + | <li class="colactive mt-1"><a href="#Ourteam">Nicotine test result of the first generation system.</a></li> | |
| − | + | <hr> | |
| − | + | <li class="mt-1"><a href="#OurSchool">E. coli growth</a></li> | |
| − | + | <hr> | |
| − | + | <li class="mt-1"><a href="#Others">Comparison between the second generation system and the original system</a></li> | |
| − | < | + | <!-- 这里是页面定位锚点 href 对应代码的 “id属性 例如 id='xxx' ”--> |
| − | </ | + | <li> |
| + | <p id="back-to-top" style="margin-top: 50px;"> | ||
| + | <a href="#top"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/e/e5/T--worldshaper-XSHS--back.png"> | ||
| + | TO Top | ||
| + | </a> | ||
| + | </p> | ||
| + | </li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-xs-10 "> | ||
| + | <div class="xshs-box3"> | ||
| + | <p class="v1" id="Ourteam" style="text-align: center; "> | ||
| + | Nicotine test result of the first generation system | ||
| + | </p> | ||
| + | <div style=" | ||
| + | width: 100%; | ||
| + | margin: 0 auto; | ||
| + | margin-top: -34px; | ||
| + | "> | ||
| + | <hr style=" | ||
| + | height: 3px; | ||
| + | background-color: #0c3d0c99!important; | ||
| + | margin-top: 40px; | ||
| + | "><div style="width:20px;"> | ||
| + | </div> | ||
| + | |||
</div> | </div> | ||
| − | + | ||
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| − | < | + | <p class="itemstyle"> |
| − | + | We measured the expression of fluorescent protein of the first version monitoring system in different concentrations of nicotine solutions. The following result showed that the growth of E. coli was not affected in a large scale when the concentration was approximately 0.001 g/L (Figure. 1) and 0.01 g/L (Figure. 2). However, the fluorescence expression intensity of the latter did not increase with the passage of time, and the effect was not obvious compared to the former concentration. In the concentration of 0.1g/L (Figure. 3), the relative expression of fluorescent protein at each time point was negative, from which we inferred that nicotine was harmful for E. coli. As we continued to increase the concentration of nicotine, the expression of fluorescent protein was getting lower and lower, which also confirmed our hypothesis that expression of fluorescent protein would abate with nicotine toxicity in certain range. | |
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| − | < | + | </p> |
| − | + | <p> | |
| − | + | <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/1/1a/T--Worldshaper-XSHS--res002.png" /> | |
| − | + | </p> | |
| − | + | <p> | |
| − | + | <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2018/e/ee/T--Worldshaper-XSHS--res003.png" /> | |
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| − | + | E. coli growth | |
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| − | + | Above-mentioned nicotine gradient shows that the expression of fluorescent protein positively correlated with the concentration of nicotine and the toxicity of nicotine itself to E. coli also cannot be ignored. We have searched for a suitable threshold for the operation of the first generation detection systems. From the following figure, the E. coli in the first version of detection system began to decrease sharply at 4h, at 0.32g/L concentration. In addition, the mathematical modeling prediction showed the same increase rate compared with the rate of decline of E. coli, according with the experimental results. | |
| − | + | </p> | |
| − | + | <p class="itemstyle"> | |
| − | + | In summary, according to mathematical modeling (with modeling), the first generation detection system suited to work in environment that the concentration of nicotine was not more than 0.32g/L and the detection time was longer than 4h. | |
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| − | + | Comparison between the second generation system and the original system | |
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| − | + | As shown below, we chose a lower concentration of nicotine (0.0001g / L). The next version of detection system could express fluorescent protein, however, only had function of detection. Compared to the first version, the expression of fluorescent protein increased significantly. The fluorescence expression of the second version of detection system was obviously higher than that of the first version. It increased greatly at 1h and reached top at 3h. Then, the fluorescence intensity dropped significantly. Thus, finally we chose the second generation system--with the recommended concentration of nicotine in the bacteria solution, 0.0001g/L, and the results obtained within 1~3h. | |
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Latest revision as of 22:29, 17 October 2018
Nicotine test result of the first generation system
We measured the expression of fluorescent protein of the first version monitoring system in different concentrations of nicotine solutions. The following result showed that the growth of E. coli was not affected in a large scale when the concentration was approximately 0.001 g/L (Figure. 1) and 0.01 g/L (Figure. 2). However, the fluorescence expression intensity of the latter did not increase with the passage of time, and the effect was not obvious compared to the former concentration. In the concentration of 0.1g/L (Figure. 3), the relative expression of fluorescent protein at each time point was negative, from which we inferred that nicotine was harmful for E. coli. As we continued to increase the concentration of nicotine, the expression of fluorescent protein was getting lower and lower, which also confirmed our hypothesis that expression of fluorescent protein would abate with nicotine toxicity in certain range.
E. coli growth
Above-mentioned nicotine gradient shows that the expression of fluorescent protein positively correlated with the concentration of nicotine and the toxicity of nicotine itself to E. coli also cannot be ignored. We have searched for a suitable threshold for the operation of the first generation detection systems. From the following figure, the E. coli in the first version of detection system began to decrease sharply at 4h, at 0.32g/L concentration. In addition, the mathematical modeling prediction showed the same increase rate compared with the rate of decline of E. coli, according with the experimental results.
In summary, according to mathematical modeling (with modeling), the first generation detection system suited to work in environment that the concentration of nicotine was not more than 0.32g/L and the detection time was longer than 4h.
Comparison between the second generation system and the original system
As shown below, we chose a lower concentration of nicotine (0.0001g / L). The next version of detection system could express fluorescent protein, however, only had function of detection. Compared to the first version, the expression of fluorescent protein increased significantly. The fluorescence expression of the second version of detection system was obviously higher than that of the first version. It increased greatly at 1h and reached top at 3h. Then, the fluorescence intensity dropped significantly. Thus, finally we chose the second generation system--with the recommended concentration of nicotine in the bacteria solution, 0.0001g/L, and the results obtained within 1~3h.
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