Difference between revisions of "Team:Valencia UPV/Collaborations"

Harvard University

Universidad de las Fuerzas Armadas ESPE

Philipps-Universität Marburg

Imperial College London

Universidad Complutense de Madrid

Ecuador

Team Project

Ecuador iGEM team has been working on the development of a new biomaterial to speed up the healing in bone injures. This biomaterial called (C-lastine), is based on the cross-linking of bacterial cellulose and Elastin-like polypeptides. This cross-linking is mediated by a carbohydrate binding domain and a BMP2 protein (an osteoblasts differentiation inducer). To achieve this objective they are using E.Coli as a biofactory, specifically C41 strain which is resistant to Cellulose synthase operon protein D, codified by gene bcsD and responsible of pore formation in the membrane allowing cellulose to exit the cell. The final result is a bandage matrix that can be applied to the wound during a surgical intervention.

Collaboration

If we think about it, Printeria is a kind of biofactory so, we thought that trying to make Ecuadorís biomaterial in Printeria would be a good trial to show that Printeria is ready to become something greater in the biotechnological industry and why not, it could be used in hospitals as well. However, due to the fact that there were problems with the size of the parts for Golden Gate and with the customs, it could not become a reality. This idea would be developed with Imperial College. Instead, we did several things.

We did a survey to get some feedback from the potential users of both projects. It consisted in a video in spanish and then a survey to fill out. In our case, the survey was made to know what users thought about Printeria and Synthetic Biology. In no case this survey represents the collective of users (only 15 people were surveyed) it was just made to have an idea.

Furthermore we provide them with a special strain called C41. This was really important for them due to the fact that without it, they could not continue with their iGEM project.

Marburg

Team Project

Do you know that there is something that grows faster than a Bacteria? If it grows faster...why not use it as a chassis to do pharmaceuticals and chemicals in the industry? This is the fantastic idea that Marburg team has developed. They want to demonstrate that working with V.Natriegens is cheaper, faster and possible with todays methods. 

Collaboration

Initially, they requested us to do the interlab study with V.natriegens and to annotate how fast V.natriegens grows but this gave us a really good idea. Why don't we try to use V.natriegens in Printeria? If all goes well, it would mean that Printeria is ready to work with other organisms which grow faster than E.coli, this way we reinforce the biofactory idea of Printeria. 

However, as things do not go as we spect in certain moments we could not make the interlab with V.natriegens. (Aunque) Marburg team gave us almost all the elements to do it we had to buy V.natriegens in the CECT and unfortunately they arrived one week before the deadline. We tried several times using a (alquilada) frozen centrifuge but 3 tries later we could not achieve electrocompetent cells. As we do not have sponsors and (alquilando) the frozen centrifuge was not cheap we stoped trying.  

on the other side, trying V.natriegens in Printeria has been an an enormous success. We achieved the (ensablaje) of (no se cuantas partes). You can see the results in the next photo:  

Imperial College

Team Project

This collaboration was born when Ismael (Imperial College iGEM team supervisor) came to the Univertitat Politécnica de Valencia to have a meeting with Diego Orzáez one of our instructors. Taking advantage of that situation we met with him and he explained us the Imperial College project called PixCell.

PicCell aims to get electronics and Synthetic Biology closer designing an innovative sistem where the culture is controlled by voltage.  

Collaboration

As they are using Golden Gate grammar we thought that it could be a good opportunity to do what we could not do with Ecuador team, try their construction in Printeria and compare results handmade and Printeria made. 

TEXTO DE SU WEB:For this year’s competition, the UPV Valencia team developed a novel microfluidic based technique to do Golden Gate construct assemblies, calling their innovative technology Printeria. When we heard about their idea through one of our supervisors, we contacted them to form a collaboration. In our collaboration, UPV Valencia assembled our 1-state and attempted to assemble our 2-state constructs, which we were having tremendous difficulty assembling ourselves. They were successful at assembling the 1-state construct using Printeria, which we were excited to compare with our manually assembled structures. The results of this collaboration can be found through this link. We would like to extend many thanks to the Valencia team for assembling constructs for us using their innovative technology.