Difference between revisions of "Team:Manchester/Parts"

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<p>Part BBa_K2613718 consists of strong promoter CP25 (works in both <i>Lactococcus lactis</i> and <i>Esherichia coli</i>) CITE, RBS (<a target="_blank" href="http://parts.igem.org/Part:BBa_B0034"/>BBa_B0034</a>),coding sequence <i>agrA</i> and <i>agrC</i> and terminators(<a target="_blank" href="http://parts.igem.org/Part:BBa_B0010"/>BBa_B0010</a> and <a target="_blank" href="http://parts.igem.org/Part:BBa_B0012"/>BBa_B0012</a>). AgrC is a transmembrane receptor histidine kinase, which detects AIP and phosphorylates/activates AgrA. AgrA is the response regulator for AgrC and drives transcription at promoter PII. AgrC and AgrA form a two-component regulatory system, which detects AIP and drives transcription at the PII promoter. In this part, AgrC has a His tag at the N-terminus to aid in protein expression detection.</p>
 
<p>Part BBa_K2613718 consists of strong promoter CP25 (works in both <i>Lactococcus lactis</i> and <i>Esherichia coli</i>) CITE, RBS (<a target="_blank" href="http://parts.igem.org/Part:BBa_B0034"/>BBa_B0034</a>),coding sequence <i>agrA</i> and <i>agrC</i> and terminators(<a target="_blank" href="http://parts.igem.org/Part:BBa_B0010"/>BBa_B0010</a> and <a target="_blank" href="http://parts.igem.org/Part:BBa_B0012"/>BBa_B0012</a>). AgrC is a transmembrane receptor histidine kinase, which detects AIP and phosphorylates/activates AgrA. AgrA is the response regulator for AgrC and drives transcription at promoter PII. AgrC and AgrA form a two-component regulatory system, which detects AIP and drives transcription at the PII promoter. In this part, AgrC has a His tag at the N-terminus to aid in protein expression detection.</p>
  
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<center><img src="https://static.igem.org/mediawiki/2018/2/24/T--Manchester--latenight1.png" width="500px" hight="auto"/></center>
  
  

Revision as of 23:11, 16 October 2018

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PARTS

Main Parts

Agr A+C - K2613741

Part BBa_K2613718 consists of strong promoter CP25 (works in both Lactococcus lactis and Esherichia coli) CITE, RBS (BBa_B0034),coding sequence agrA and agrC and terminators(BBa_B0010 and BBa_B0012). AgrC is a transmembrane receptor histidine kinase, which detects AIP and phosphorylates/activates AgrA. AgrA is the response regulator for AgrC and drives transcription at promoter PII. AgrC and AgrA form a two-component regulatory system, which detects AIP and drives transcription at the PII promoter. In this part, AgrC has a His tag at the N-terminus to aid in protein expression detection.

Part Table

<groupparts>iGEM18 Manchester</groupparts>