Difference between revisions of "Team:Manchester/Parts"
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<h3>Main Parts</h3> | <h3>Main Parts</h3> | ||
| − | <center><h4>Agr A+C - K2613741</h4></center> | + | <center><h4><i>Agr</i> <i>A</i>+<i>C</i> - K2613741</h4></center> |
<p>Part BBa_K2613718 consists of strong promoter CP25 (works in both <i>Lactococcus lactis</i> and <i>Esherichia coli</i>) CITE, RBS (<a target="_blank" href="http://parts.igem.org/Part:BBa_B0034"/>BBa_B0034</a>),coding sequence <i>agrA</i> and <i>agrC</i> and terminators(<a target="_blank" href="http://parts.igem.org/Part:BBa_B0010"/>BBa_B0010</a> and <a target="_blank" href="http://parts.igem.org/Part:BBa_B0012"/>BBa_B0012</a>). AgrC is a transmembrane receptor histidine kinase, which detects AIP and phosphorylates/activates AgrA. AgrA is the response regulator for AgrC and drives transcription at promoter PII. AgrC and AgrA form a two-component regulatory system, which detects AIP and drives transcription at the PII promoter. In this part, AgrC has a His tag at the N-terminus to aid in protein expression detection.</p> | <p>Part BBa_K2613718 consists of strong promoter CP25 (works in both <i>Lactococcus lactis</i> and <i>Esherichia coli</i>) CITE, RBS (<a target="_blank" href="http://parts.igem.org/Part:BBa_B0034"/>BBa_B0034</a>),coding sequence <i>agrA</i> and <i>agrC</i> and terminators(<a target="_blank" href="http://parts.igem.org/Part:BBa_B0010"/>BBa_B0010</a> and <a target="_blank" href="http://parts.igem.org/Part:BBa_B0012"/>BBa_B0012</a>). AgrC is a transmembrane receptor histidine kinase, which detects AIP and phosphorylates/activates AgrA. AgrA is the response regulator for AgrC and drives transcription at promoter PII. AgrC and AgrA form a two-component regulatory system, which detects AIP and drives transcription at the PII promoter. In this part, AgrC has a His tag at the N-terminus to aid in protein expression detection.</p> | ||
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| + | <center><h4><i>Agr</i> <i>B</i>+<i>D</i> – K2613743</h4></center> | ||
| − | + | <p>Part BBa_K2613743 consists of strong promoter pTet, RBS (<a target="_blank" href="http://parts.igem.org/Part:BBa_B0034"/>BBa_B0034</a>), coding sequence <i>agrB</i> and <i>agrD</i> and terminators(<a target="_blank" href="http://parts.igem.org/Part:BBa_B0010"/>BBa_B0010</a> and <a target="_blank" href="http://parts.igem.org/Part:BBa_B0012"/>BBa_B0012</a>). Expression of <i>agrB</i> and <i>agrD</i> is regulated by TetR. AgrB is an integral membrane protein; AgrD is a short peptide that is transported outside of the cell by AgrB. During transportation, AgrD is covalently modified to produce a small autoinducing peptide (AIP) which can be taken up by cells and induces transcription of genes under control of PII promoter.</p> | |
| − | + | ||
Revision as of 23:16, 16 October 2018
PARTS
Main Parts
Agr A+C - K2613741
Part BBa_K2613718 consists of strong promoter CP25 (works in both Lactococcus lactis and Esherichia coli) CITE, RBS (BBa_B0034),coding sequence agrA and agrC and terminators(BBa_B0010 and BBa_B0012). AgrC is a transmembrane receptor histidine kinase, which detects AIP and phosphorylates/activates AgrA. AgrA is the response regulator for AgrC and drives transcription at promoter PII. AgrC and AgrA form a two-component regulatory system, which detects AIP and drives transcription at the PII promoter. In this part, AgrC has a His tag at the N-terminus to aid in protein expression detection.
Agr B+D – K2613743
Part BBa_K2613743 consists of strong promoter pTet, RBS (BBa_B0034), coding sequence agrB and agrD and terminators(BBa_B0010 and BBa_B0012). Expression of agrB and agrD is regulated by TetR. AgrB is an integral membrane protein; AgrD is a short peptide that is transported outside of the cell by AgrB. During transportation, AgrD is covalently modified to produce a small autoinducing peptide (AIP) which can be taken up by cells and induces transcription of genes under control of PII promoter.
Part Table
<groupparts>iGEM18 Manchester</groupparts>