Difference between revisions of "Team:Manchester/Parts"

 
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<h1 style="line-height: 1.5; text-align: center; border-bottom: none; "><b>PARTS</b></h1>
  
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<h1>Parts</h1>
 
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
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<h3>Main Parts</h3>
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<center><h4><i>Agr</i> <i>A</i>+<i>C</i> - K2613741</h4></center>
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<p>Part BBa_K2613718 consists of strong promoter CP25 (works in both <i>Lactococcus lactis</i> and <i>Esherichia coli</i>) (<a target="_blank" href="https://aem.asm.org/content/aem/64/1/82.full.pdf"/>Jensen and Hammer’s 1998 paper</a>), RBS (<a target="_blank" href="http://parts.igem.org/Part:BBa_B0034"/>BBa_B0034</a>),coding sequence <i>agrA</i> and <i>agrC</i> and terminators(<a target="_blank" href="http://parts.igem.org/Part:BBa_B0010"/>BBa_B0010</a> and <a target="_blank" href="http://parts.igem.org/Part:BBa_B0012"/>BBa_B0012</a>). AgrC is a transmembrane receptor histidine kinase, which detects AIP and phosphorylates/activates AgrA. AgrA is the response regulator for AgrC and drives transcription at promoter PII. AgrC and AgrA form a two-component regulatory system, which detects AIP and drives transcription at the PII promoter. In this part, AgrC has a His tag at the N-terminus to aid in protein expression detection.</p>
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<center><img src="https://static.igem.org/mediawiki/2018/2/24/T--Manchester--latenight1.png" width="500px" hight="auto"/></center>
  
  
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<center><h4><i>Agr</i> <i>B</i>+<i>D</i> – K2613743</h4></center>
  
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<p>Part BBa_K2613743 consists of strong promoter pTet, RBS (<a target="_blank" href="http://parts.igem.org/Part:BBa_B0034"/>BBa_B0034</a>), coding sequence <i>agrB</i> and <i>agrD</i> and terminators(<a target="_blank" href="http://parts.igem.org/Part:BBa_B0010"/>BBa_B0010</a> and <a target="_blank" href="http://parts.igem.org/Part:BBa_B0012"/>BBa_B0012</a>). Expression of <i>agrB</i> and <i>agrD</i> is regulated by TetR. AgrB is an integral membrane protein; AgrD is a short peptide that is transported outside of the cell by AgrB. During transportation, AgrD is covalently modified to produce a small autoinducing peptide (AIP) which can be taken up by cells and induces transcription of genes under control of PII promoter.</p>
  
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<center><img src="https://static.igem.org/mediawiki/2018/3/36/T--Manchester--latenight2.png" width="650px" hight="auto"/></center>
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<center><h4>PII - K2613740</h4></center>
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<p>Part BBa_K2613740 consists of <i>amilCP</i> reporter under control of PII promoter, RBS(<a target="_blank" href="http://parts.igem.org/Part:BBa_B0034"/>BBa_B0034</a>) and terminators(<a target="_blank" href="http://parts.igem.org/Part:BBa_B0010"/>BBa_B0010</a> and <a target="_blank" href="http://parts.igem.org/Part:BBa_B0012"/>BBa_B0012</a>). PII promoter is a promoter taken from the <i>Listeria monocytogenes</i> genome which is involved in quorum sensing. When activated AgrA binds to PII, Transcription can occur (<a target="_blank" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916163/"/>Zetzmann, M. <i>et al.</i> 2016</a>). This means that when this device detects activated AgrA, AmilCP reporter protein is produced.</p>
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<center><img src="https://static.igem.org/mediawiki/2018/3/35/T--Manchester--latenight3.png" width="400px" hight="auto"/></center>
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<h3><u>References</u></h3>
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<p> 1. Zetzmann, M., Sánchez-Kopper, A., Waidmann, M., Blombach, B. and Riedel, C. (2016). Identification of the agr Peptide of Listeria monocytogenes. Frontiers in Microbiology, 7 <br>
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2. Jensen, P.R. and Hammer, K., 1998. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Applied and environmental microbiology, 64(1), pp.82-87.</p>
  
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<h3>Adding parts to the registry</h3>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
  
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
 
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ADD PARTS
 
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<h3>Inspiration</h3>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
  
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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<h3>What information do I need to start putting my parts on the Registry?</h3>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<li>Part Name</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
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<h3>Part Table </h3>
 
<h3>Part Table </h3>
  
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM18 Manchester</groupparts>
 
<groupparts>iGEM18 Manchester</groupparts>
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{{Manchester/Footer}}
 
{{Manchester/Footer}}

Latest revision as of 03:41, 18 October 2018

HOME
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TEAM




PARTS

Main Parts

Agr A+C - K2613741

Part BBa_K2613718 consists of strong promoter CP25 (works in both Lactococcus lactis and Esherichia coli) (Jensen and Hammer’s 1998 paper), RBS (BBa_B0034),coding sequence agrA and agrC and terminators(BBa_B0010 and BBa_B0012). AgrC is a transmembrane receptor histidine kinase, which detects AIP and phosphorylates/activates AgrA. AgrA is the response regulator for AgrC and drives transcription at promoter PII. AgrC and AgrA form a two-component regulatory system, which detects AIP and drives transcription at the PII promoter. In this part, AgrC has a His tag at the N-terminus to aid in protein expression detection.

Agr B+D – K2613743

Part BBa_K2613743 consists of strong promoter pTet, RBS (BBa_B0034), coding sequence agrB and agrD and terminators(BBa_B0010 and BBa_B0012). Expression of agrB and agrD is regulated by TetR. AgrB is an integral membrane protein; AgrD is a short peptide that is transported outside of the cell by AgrB. During transportation, AgrD is covalently modified to produce a small autoinducing peptide (AIP) which can be taken up by cells and induces transcription of genes under control of PII promoter.

PII - K2613740

Part BBa_K2613740 consists of amilCP reporter under control of PII promoter, RBS(BBa_B0034) and terminators(BBa_B0010 and BBa_B0012). PII promoter is a promoter taken from the Listeria monocytogenes genome which is involved in quorum sensing. When activated AgrA binds to PII, Transcription can occur (Zetzmann, M. et al. 2016). This means that when this device detects activated AgrA, AmilCP reporter protein is produced.

References

1. Zetzmann, M., Sánchez-Kopper, A., Waidmann, M., Blombach, B. and Riedel, C. (2016). Identification of the agr Peptide of Listeria monocytogenes. Frontiers in Microbiology, 7
2. Jensen, P.R. and Hammer, K., 1998. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Applied and environmental microbiology, 64(1), pp.82-87.

Part Table

<groupparts>iGEM18 Manchester</groupparts>