Difference between revisions of "Team:Manchester"

 
(157 intermediate revisions by 4 users not shown)
Line 1: Line 1:
{{Manchester}}
+
 
 
{{Manchester/Navbar}}
 
{{Manchester/Navbar}}
  
Line 11: Line 11:
 
<meta name="author" content="igemmanchester">
 
<meta name="author" content="igemmanchester">
 
<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
 
<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
 
<!------LINK THE CSS FILES--->
 
<link rel="stylesheet" href="css/bootstrap.min.css">
 
<link rel="stylesheet" href="css/animate.css">
 
<link rel="stylesheet" href="css/magnific-popup.css">
 
<link rel="stylesheet" href="css/font-awesome.min.css">
 
  
 
<!-- Main css -->
 
<!-- Main css -->
<link rel="stylesheet" href="css/tooplate-style.css">
+
<link rel="stylesheet" href="https://2018.igem.org/Team:Manchester/CSS">
 
</head>
 
</head>
<title> The University of Man-cheeseter</title>
+
<title> The University of Man-cheester</title>
 
<body>
 
<body>
<!-- PRE LOADER -->
 
<div class="preloader">
 
    <div class="spinner">
 
          <span class="spinner-rotate"></span>
 
    </div>
 
</div>
 
  
<!-- HOME SECTION -->
 
<div class="container">
 
    <div class="row">
 
          <section id="home" class="parallax-section">
 
  
              <div class="col-md-offset-1 col-md-10 col-sm-12">
 
                    <div class="home-wrapper">
 
                        <h3 class="wow fadeInUp" data-wow-delay="0.4s">Listeria Biosensor for soft cheese</h3>
 
                        <h1 class="wow fadeInUp" data-wow-delay="0.6s">MAN-CHEESTER</h1>
 
                        <a href="#about" class="wow fadeInUp smoothScroll arrow-btn" data-wow-delay="1s"><i class="fa fa-angle-double-down"></i></a>
 
                    </div>
 
              </div>
 
  
          </section>
+
<h1> FIGHTING <i>LISTERIA</i> HYSTERIA</h1>
    </div>
+
 
 +
 
 +
</div>
 
</div>
 
</div>
  
<!-- ABOUT SECTION -->
+
<center><img src="https://static.igem.org/mediawiki/2018/0/01/T--Manchester--Homepage1.jpeg"></center>
<section id="about" class="parallax-section">
+
    <div class="container">
+
          <div class="row">
+
  
              <div class="col-md-8 col-sm-12">
+
<div id="abstract">
                    <div class="about-thumb">
+
  <h2>ABSTRACT</h2>
                        <div class="wow fadeInUp section-title" data-wow-delay="0.6s">
+
                              <h3>ABSTRACT</h3>
+
                              <h2>Development of a soft cheese with integrated <i>Listeria monocytogenes</i> biosensor</h2>
+
                        </div>
+
                        <div class="wow fadeInUp" data-wow-delay="0.8s">
+
                              <p>Listeria monocytogenes is a gram-positive, rod-shaped, food-borne bacterium, capable of causing the rare but potentially fatal disease listeriosis. L.monocytogenes can replicate at temperatures as low as 0°C allowing it to survive in industrial and domestic refrigerators. L.monocytogenes can often grow in soft cheeses, making many varieties of cheese unavailable to those who are immunosuppressed. Man-Cheester aims to modify Lactococcus lactis, a common bacterium in cheese manufacture. We will transform L. lactis in a cheese starter culture to detect AIP, a key quorum signalling molecule produced by L. monocytogenes. This will be done by expressing the agr quorum-sensing system used by L.monocytogenes in L. lactis. On detection of AIP, a colour change will occur within L. lactis causing the cheese to turn purple, alerting the consumer to its contamination. Our concept could be further developed to include other sources of L. monocytogenes contamination such as meats and vegetables to prevent as many cases of listeriosis as possible.</p>
+
                        </div>
+
                    </div>
+
              </div>
+
  
          </div>
+
<p><i><b>Listeria monocytogenes</b></i> is a Gram-positive, rod-shaped, food-borne bacterium, capable of causing the rare, but potentially fatal, disease <b>listeriosis</b>. <i>L. monocytogenes</i> can replicate at temperatures as low as 0°C, allowing it to survive in industrial and domestic refrigerators. <i>L. monocytogenes</i> is often found in soft cheeses, making many varieties of cheese unavailable to those who are immunosuppressed. <b>MAN-CHEESTER</b> aims to introduce the <i>agr</i> quorum sensing system from <i>L. monocytogenes</i> into bacteria used in the cheese making process. On detection of AIP, a key quorum sensing molecule of <i>L. monocytogenes</i>, a colour change will occur, causing the cheese to turn purple and alerting the consumer to its contamination. Our concept could be further developed to include other sources of <i>L. monocytogenes</i> contamination, such as meats, vegetables or kitchen surfaces, to prevent as many cases of listeriosis as possible.</p>
    </div>
+
</section>
+
  
 +
<br>
  
<!-- WORK SECTION -->
+
<center><button class="btn5"><a style="text-decoration:none; color: white"; width="auto" href="https://2018.igem.org/Team:Manchester/Description">Read more</a></button></center>
<section id="work" class="parallax-section">
+
    <div class="container">
+
          <div class="row">
+
  
              <div class="wow fadeInUp col-md-3 col-sm-6" data-wow-delay="0.4s">
+
<br>
                    <!-- WORK THUMB -->
+
<br>
                    <div class="work-thumb">
+
<center><button class="btn1"><a style="text-decoration:none; color: white" href="https://2018.igem.org/Team:Manchester/Experiments">Wet Lab</a></button> &nbsp; &nbsp; &nbsp; &nbsp;
                        <a href="images/work-1.jpg" class="image-popup">
+
                              <img src="images/work-1.jpg" class="img-responsive" alt="Fine Arts">
+
                        </a>
+
                    </div>
+
                    <h4>Wet lab</h4>
+
              </div>
+
  
              <div class="wow fadeInUp col-md-3 col-sm-6" data-wow-delay="0.6s">
 
                    <!-- WORK THUMB -->
 
                    <div class="work-thumb">
 
                        <a href="images/work-2.jpg" class="image-popup">
 
                              <img src="images/work-2.jpg" class="img-responsive" alt="Logo Design">
 
                        </a>
 
                    </div>
 
                    <h4>Dry lab</h4>
 
              </div>
 
  
              <div class="wow fadeInUp col-md-3 col-sm-6" data-wow-delay="0.8s">
+
<button class="btn2"><a style="text-decoration:none; color: black" href="https://2018.igem.org/Team:Manchester/Model">Dry Lab</a></button>&nbsp; &nbsp; &nbsp; &nbsp;
                    <!-- WORK THUMB -->
+
                    <div class="work-thumb">
+
                        <a href="images/work-3.jpg" class="image-popup">
+
                              <img src="images/work-3.jpg" class="img-responsive" alt="Photography">
+
                        </a>
+
                    </div>
+
                    <h4>Human Practices</h4>
+
              </div>
+
  
              <div class="wow fadeInUp col-md-3 col-sm-6" data-wow-delay="0.8s">
 
                    <!-- WORK THUMB -->
 
                    <div class="work-thumb">
 
                        <a href="https://2018.igem.org/Team:Manchester/Team" class="image-popup">
 
                              <img src="images/work-4.jpg" class="img-responsive" alt="https://2018.igem.org/Team:Manchester/Team">
 
                        </a>
 
                    </div>
 
                    <h4>Team</h4>
 
              </div>
 
  
          </div>
+
<button class="btn3"><a style="text-decoration:none; color: #62628c" href="https://2018.igem.org/Team:Manchester/Human_Practices">Human Practices</a></button> &nbsp; &nbsp; &nbsp; &nbsp;
    </div>
+
 
</section>
+
<button class="btn4"><a style="text-decoration:none; color: white" href="https://2018.igem.org/Team:Manchester/Results">Achievements</a></button></center> &nbsp; &nbsp; &nbsp; &nbsp;
 +
 
  
<!-----OLD SECTION DELETE WHEN HPG SORTED--->
 
<div id="main">
 
<p2><i>Listeria monocytogenes</i> biosensor for soft cheeses</p2>
 
<div id="mainpgpic">
 
        <img src="https://2018.igem.org/wiki/image/T--Manchester--Softcheese_homescreenimg.jpg" width="1000" height="600">
 
 
</div>
 
</div>
  
<div id="abstract">
+
 
                                        <h2>Abstract</h2>
+
 
<p1><i>Listeria monocytogenes </i> is a gram-positive, rod-shaped, food-borne bacterium, capable of causing the rare but potentially fatal disease listeriosis. L.monocytogenes can replicate at temperatures as low as 0°C allowing it to survive in industrial and domestic refrigerators. <i>L.monocytogenes</i> can often grow in soft cheeses, making many varieties of cheese unavailable to those who are immunosuppressed. Man-Cheester aims to modify <i>Lactococcus lactis</i>, a common bacterium in cheese manufacture. We will transform <i>L. lactis</i> in a cheese starter culture to detect AIP, a key quorum signalling molecule produced by <i>L. monocytogenes</i>. This will be done by expressing the agr quorum-sensing system used by <i>L.monocytogenes</i> in <i>L. lactis</i>. On detection of AIP, a colour change will occur within <i>L. lactis</i> causing the cheese to turn purple, alerting the consumer to its contamination. Our concept could be further developed to include other sources of <i>L. monocytogenes</i>  contamination such as meats and vegetables to prevent as many cases of listeriosis as possible.</p1>
+
</div>
</div>
+
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
  
 
</body>
 
</body>
 
<style>
 
<style>
p2{
+
 
    text-align: center;
+
 
    font-size: 40px;
+
 
 +
 
 +
a {
 +
text-decoration: none;  
 
}
 
}
  
h2 {
+
h1 {
    text-align: center;
+
border-bottom: none;
    font-size: 20px;
+
text-align: center;
    padding: 30px;
+
padding-top: 7%;
 +
color: #47476b;
 +
}
 +
 
 +
h3 {
 +
border-bottom: none;!important
 +
text-align: center;
 +
color: #62628c;
 +
}
 +
 
 +
h2{
 +
text-align: center;
 +
color: #52527a;
 +
text-decoration: none;
 +
}
 +
 
 +
   
 +
 
}
 
}
  
 
#main {
 
#main {
 
     text-align: center;
 
     text-align: center;
    padding: 20px;
 
 
}
 
}
 +
 
#mainpgpic {
 
#mainpgpic {
 
   text-align: center;
 
   text-align: center;
Line 152: Line 105:
  
 
p1 {
 
p1 {
  text-align: relative;
+
 
  Font-size: 20px;
+
    text-align: justify;
  line-height: 20px;
+
    text-justify: inter-word;
 +
    Font-size: 20px;
 +
    line-height: 2.6em;
 
}
 
}
  
 
#abstract {
 
#abstract {
   padding-left: 100px;
+
   text-align: justify;
   padding-right: 100px;
+
   background-colour: white;  
padding-bottom: 50px;
+
  padding-right:10%;
  text-align: justified;
+
padding-left:10%;
  background-color: white;
+
padding-bottom:10%;
 +
 
 
}
 
}
 +
 +
.btn2 {
 +
height: 250px;
 +
width: 250px;
 +
background:transparent url('https://static.igem.org/mediawiki/2018/d/d1/T--Manchester--DryLabButton2.jpeg') no-repeat;
 +
border-radius: 75px;
 +
border: 0;
 +
overflow: hidden;
 +
color: black;
 +
alignment: center;
 +
transition-duration: 0.6s;
 +
padding: 10%
 +
/* IE 6/7 fallback */
 +
*text-indent: -9999px;
 +
font-size: 300%;
 +
}
 +
 +
.btn2:hover {
 +
    background: transparent url('https://static.igem.org/mediawiki/2018/9/96/T--Manchester--DryLabButton1.jpeg') no-repeat;
 +
    color: black;
 +
}
 +
 +
 +
.btn1 {
 +
height: 250px;
 +
width: 250px;
 +
background:transparent url('https://static.igem.org/mediawiki/2018/e/e9/T--Manchester--WetLabButton2.jpeg') no-repeat;
 +
border-radius: 75px;
 +
border: 0;
 +
overflow: hidden;
 +
color: white;
 +
alignment: center;
 +
transition-duration: 0.6s;
 +
padding: 10%
 +
/* IE 6/7 fallback */
 +
*text-indent: -9999px;
 +
font-size: 300%;
 +
}
 +
 +
.btn1:hover {
 +
    background: transparent url('https://static.igem.org/mediawiki/2018/a/a6/T--Manchester--WetLabButton1.jpeg') no-repeat;
 +
    color:white;
 +
}
 +
 +
 +
.btn3 {
 +
height: 250px;
 +
width: 250px;
 +
background:transparent url('https://static.igem.org/mediawiki/2018/2/29/T--Manchester--HPButton2.jpeg') no-repeat;
 +
border-radius: 75px;
 +
border: 0;
 +
overflow: hidden;
 +
color: white;
 +
alignment: center;
 +
transition-duration: 0.6s;
 +
padding: 10%
 +
/* IE 6/7 fallback */
 +
*text-indent: -9999px;
 +
font-size: 250%;
 +
}
 +
 +
.btn3:hover {
 +
    background: transparent url('https://static.igem.org/mediawiki/2018/9/91/T--Manchester--HPButton1.jpeg') no-repeat;
 +
    color:white;
 +
}
 +
 +
 +
.btn4 {
 +
height: 250px;
 +
width: 250px;
 +
background:transparent url('https://static.igem.org/mediawiki/2018/8/89/T--Manchester--TeamButton2.jpeg') no-repeat;
 +
border-radius: 75px;
 +
border: 0;
 +
overflow: hidden;
 +
color: white;
 +
alignment: center;
 +
transition-duration: 0.6s;
 +
padding: 10%
 +
/* IE 6/7 fallback */
 +
*text-indent: -9999px;
 +
font-size: 280%;
 +
}
 +
 +
.btn4:hover {
 +
    background: transparent url('https://static.igem.org/mediawiki/2018/5/59/T--Manchester--TeamButton1.jpeg') no-repeat;
 +
    color:white;
 +
}
 +
 +
 +
 +
.btn5 {
 +
height: 100px;
 +
width: 1100px;
 +
background:goldenrod;
 +
border-radius: 75px;
 +
border: 0;
 +
overflow: hidden;
 +
color: white;
 +
text-align: center;
 +
transition-duration: 0.6s;
 +
font-size: 300%;
 +
}
 +
 +
.btn5:hover {
 +
    background: #c6961d;
 +
    color:white;
 +
}
 +
 
</style>
 
</style>
 +
 
</html>
 
</html>
 
{{Manchester/Footer}}
 
{{Manchester/Footer}}

Latest revision as of 14:35, 9 November 2018


Man-Cheester The University of Man-cheester

FIGHTING LISTERIA HYSTERIA

ABSTRACT

Listeria monocytogenes is a Gram-positive, rod-shaped, food-borne bacterium, capable of causing the rare, but potentially fatal, disease listeriosis. L. monocytogenes can replicate at temperatures as low as 0°C, allowing it to survive in industrial and domestic refrigerators. L. monocytogenes is often found in soft cheeses, making many varieties of cheese unavailable to those who are immunosuppressed. MAN-CHEESTER aims to introduce the agr quorum sensing system from L. monocytogenes into bacteria used in the cheese making process. On detection of AIP, a key quorum sensing molecule of L. monocytogenes, a colour change will occur, causing the cheese to turn purple and alerting the consumer to its contamination. Our concept could be further developed to include other sources of L. monocytogenes contamination, such as meats, vegetables or kitchen surfaces, to prevent as many cases of listeriosis as possible.