Line 47: | Line 47: | ||
<ul> | <ul> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Team">Members</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Collaborations">Collaborations</a> |
</li> | </li> | ||
</ul> | </ul> | ||
Line 66: | Line 66: | ||
<ul> | <ul> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Background.html">Background</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/design.html">Design</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Protocols-methods.html">Protocols and methods</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/result.html">Results</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Model">Model</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Demonstrate">Demonstrate</a> |
</li> | </li> | ||
Line 97: | Line 97: | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/parts.html">Parts</a> |
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
Line 107: | Line 107: | ||
<ul> | <ul> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/HP/Silver">Silver</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/HP/Gold_Integrated">Integrated and Gold</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Engagement">Engagement</a> |
</li> | </li> | ||
</ul> | </ul> | ||
Line 119: | Line 119: | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Applied_Design">Applied Design</a> |
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
Line 129: | Line 129: | ||
<ul> | <ul> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/Medal-requirements.html">Medal requirements</a> |
</li> | </li> | ||
</ul> | </ul> | ||
Line 135: | Line 135: | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:Worldshaper-XSHS/safety.html">Safety</a> |
</li> | </li> | ||
</ul> | </ul> |
Revision as of 03:36, 5 October 2018
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report.
Abstract