<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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The Fifth InterLab study
The Fifth InterLab study
Measuring the GFP expression is a useful tool worldwide for quantifying gene expression. Currently, there are various differences in fluorescence data which is measured on different devices (e.g. different units of fluorescence recorded or different settings of the data processing used). The aim of the Fifth InterLab study is to make fluorescence measurements more compatible among various laboratories around the world. This is to be done by standardising the protocol for the machine calibration with Silica beads and relating OD600 values with colony forming units (CFUs). The expression of the GFP by one cell will be calculated by dividing the fluorescence of a population of cells via CFUs instead of using an OD600 measurement which is less precise.
Measuring the GFP expression is a useful tool worldwide for quantifying gene expression. Currently, there are various differences in fluorescence data which is measured on different devices (e.g. different units of fluorescence recorded or different settings of the data processing used). The aim of the Fifth InterLab study is to make fluorescence measurements more compatible among various laboratories around the world. This is to be done by standardising the protocol for the machine calibration with Silica beads and relating OD600 values with colony forming units (CFUs). The expression of the GFP by one cell will be calculated by dividing the fluorescence of a population of cells via CFUs instead of using an OD600 measurement which is less precise.
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<h1>InterLab</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
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For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
The Fifth InterLab study
Measuring the GFP expression is a useful tool worldwide for quantifying gene expression. Currently, there are various differences in fluorescence data which is measured on different devices (e.g. different units of fluorescence recorded or different settings of the data processing used). The aim of the Fifth InterLab study is to make fluorescence measurements more compatible among various laboratories around the world. This is to be done by standardising the protocol for the machine calibration with Silica beads and relating OD600 values with colony forming units (CFUs). The expression of the GFP by one cell will be calculated by dividing the fluorescence of a population of cells via CFUs instead of using an OD600 measurement which is less precise.
