Difference between revisions of "Team:Manchester/Results"

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<li><p>Designed five new BioBricks: three codon-optimised for expression of the agr quorum sensing system in <i>E. coli</i> and <i>L. lactis</i>; two to test new cross-chassis promoters.  
 
<li><p>Designed five new BioBricks: three codon-optimised for expression of the agr quorum sensing system in <i>E. coli</i> and <i>L. lactis</i>; two to test new cross-chassis promoters.  
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<li><p>Showed further characterisation of CP25 promoter from Jensen and Hammer, 1998</p></li>
 
<li><p>Showed further characterisation of CP25 promoter from Jensen and Hammer, 1998</p></li>
 
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<p>Built two new models as part of the project from two stand-points:
 
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<h2>Human Practices</h2>
 
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<li><p>Carried out in depth literature research into recent news articles about the impact of listeriosis</p></li>
 
<li><p>Carried out in depth literature research into recent news articles about the impact of listeriosis</p></li>
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Revision as of 01:47, 18 October 2018





ACHIEVEMENTS

Bronze

✔ Registration for Jamboree and Jamboree attendance done

✔ Competition deliverables: Wiki, Poster, Presentation, Judging Form

✔ Complete Attributions page

✔ Successfully complete the InterLab study and document it on the Wiki

Silver

✔ Submit validated part to the iGEM registry (BBa_K2613743)

✔ Convince the judges you have thought carefully about whether your work is responsible through Human Practices

Collaborate with one (or more) other iGEM team(s)

Gold

✔ Demonstrate how you have integrated investigated Human Practices issues into the purpose, design and/or execution of your project

Model your project




Wet Lab

  • Designed five new BioBricks: three codon-optimised for expression of the agr quorum sensing system in E. coli and L. lactis; two to test new cross-chassis promoters.

  • Validated and submitted one new BioBrick to the registry.

  • Showed further characterisation of CP25 promoter from Jensen and Hammer, 1998

Dry Lab

Built two new models as part of the project from two stand-points:

  • Promoter optimisation tool: a valuable focused model for the iGEM community to optimise promoters when considering moving genetic parts across species

  • Collaborated on promoter optimisation tool internationally to provide novel promoter sequences to other iGEM teams

  • Agr operon model: a broader range model to give insight into how diffusion of small signalling molecules could occur in medium with unusual physical properties (cheese)

Human Practices

  • Carried out in depth literature research into recent news articles about the impact of listeriosis

  • Talked with relevant international industry partners on the design of our project

  • Investigated how Britain leaving the EU would affect the feasibility of our project through literature research and discussions with the European Commission

  • Carried out public engagement events to promote synthetic biology as a tool to produce safer foodstuffs

  • Used feedback from all human practices activities to influence the design and execution of our project