Team:NCHU Taichung/Parts

NCHU_Taichung

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Parts Overview

Parts

Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene and replace the ori and also the resistance gene, which can also be seen in the basic part.

Basic Parts

  1. BBa_K2546001: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of halogenated compounds
  2. BBa_K2546004: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
  3. BBa_K2546007: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
  4. BBa_K2546008: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the Ori of pBBR1 and BBa_J364007 in pSB1C3.
  5. BBa_K2546010: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion carrier, siderophore, which is similar in structure with dioxin.
  6. BBa_K2546011: This part is coding for Laccase, which is related to lignin degradation.

Intermediate Parts

  1. BBa_K2546002: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding site.

Composite Parts

  1. BBa_K2546005: This part is the promoter circuit of BBa_K2546002.
  2. BBa_K2546006: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to let three gene be expressed in one circuit.
  3. BBa_K2546012: This part is the promoter circuit of BBa_K2546010.

Name Type Description Designer Length
BBa_K2546000 Coding dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf Tsu-Wang Sun
BBa_K2546001 Coding 2-haloacid dehalogenase(HAD) Tsu-Wang Sun 780
BBa_K2546002 Coding 2-haloacid dehalogenase plus laccase with robosome binding site Tsu-Wang Sun 2408
BBa_K2546003 Coding p+HL Tsu-Wang Sun 6
BBa_K2546004 Coding pME60C shuttle vector Tsu-Wang Sun 6489
BBa_K2546005 Coding 2-haloacid dehalogenase plus laccase with promoter and ribosome binding site Tsu-Wang Sun 2451
BBa_K2546006 Coding TonB + 2-haloacid dehalogenase + laccase Tsu-Wang Sun 4721
BBa_K2546007 Plasmid_Backbone cm 4657 Tsu-Wang Sun 770
BBa_K2546008 Plasmid_Backbone pME60C2 shuttle vector Tsu-Wang Sun 5491
BBa_K2546009 Plasmid_Backbone kan bbrf2 Tsu-Wang Sun 4218
BBa_K2546010 Coding TonB-dependent receptor (TonB) Tsu-Wang Sun 2244
BBa_K2546011 Coding Laccase (TLcc) Tsu-Wang Sun 1584
BBa_K2546012 Coding TonB with promoter and ribosome binding site Yu-Hsi Lin 2305