Difference between revisions of "Team:NCHU Taichung/Parts"

 
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{{NCHU_Taichung/navbar}}
 
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<div class="ui main text container">
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<groupparts>iGEM18 NCHU_Taichung</groupparts>
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      <div class="ui raised segment" id="mainbox">
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        <h1 class="ui hidden header"></h1>
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        <div class="ui huge header" id="top">Parts Overview</div>
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        <h2 class="ui header">Parts</h2>
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        <p class="longP">Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase
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          DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part
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          of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene
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          and replace the ori and also the resistance gene, which can also be seen in the basic part. </p>
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        <h3 class="ui dividing header">Basic Parts</h3>
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        <ol class="ui ordered list">
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546001  BBa_K2546001:] This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of
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            halogenated compounds</li>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546004  BBa_K2546004:] This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
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            of pMP4657 and BBa_J364007 in pSB1C3. </li>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546007  BBa_K2546007:] This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
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            of pMP4657 and BBa_J364007 in pSB1C3. </li>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546008  BBa_K2546008:] This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the
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            Ori of pBBR1 and BBa_J364007 in pSB1C3. </li>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546010  BBa_K2546010:] This part is coding for TonB-dependent receptor, which can actively transport the ferric ion
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            carrier, siderophore, which is similar in structure with dioxin.</li>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546011  BBa_K2546011:] This part is coding for Laccase, which is related to lignin degradation.</li>
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        </ol>
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        <h3 class="ui dividing header">Intermediate Parts</h3>
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        <ol class="ui ordered list">
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546002  BBa_K2546002:] This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding
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            site.</li>
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        </ol>
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        <h3 class="ui dividing header">Composite Parts</h3>
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        <ol class="ui ordered list">
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546005  BBa_K2546005:] This part is the promoter circuit of BBa_K2546002.</li>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546006  BBa_K2546006:] This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to
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            let three gene be expressed in one circuit.</li>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546012  BBa_K2546012:] This part is the promoter circuit of BBa_K2546010.</li>
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        </ol>
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        <h1 class="ui hidden header"></h1>
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        <table class="ui selectable teal celled small table">
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            <tr>
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              <th class="three wide">Name</th>
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              <th class="three wide">Type</th>
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              <th class="four wide">Description</th>
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              <th class="three wide">Designer</th>
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              <th class="three wide">Length</th>
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            </tr>
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            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546000  BBa_K2546000 ]              </td>
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              <td>Coding </td>
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              <td>dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf </td>
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              <td>Tsu-Wang Sun </td>
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              <td> </td>
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            </tr>
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            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546001  BBa_K2546001 ]              </td>
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              <td>Coding </td>
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              <td>2-haloacid dehalogenase(HAD) </td>
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              <td>Tsu-Wang Sun </td>
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              <td>780</td>
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            </tr>
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            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546002  BBa_K2546002 ]              </td>
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              <td>Coding </td>
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              <td>2-haloacid dehalogenase plus laccase with robosome binding site </td>
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              <td>Tsu-Wang Sun </td>
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              <td>2408</td>
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            </tr>
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            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546003  BBa_K2546003 ]              </td>
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              <td>Coding </td>
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              <td>p+HL </td>
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              <td>Tsu-Wang Sun </td>
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              <td>6</td>
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            </tr>
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            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546004  BBa_K2546004 ]              </td>
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              <td>Coding </td>
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              <td>pME60C shuttle vector </td>
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              <td>Tsu-Wang Sun </td>
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              <td>6489</td>
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            </tr>
 +
            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546005  BBa_K2546005 ]              </td>
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              <td>Coding </td>
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              <td>2-haloacid dehalogenase plus laccase with promoter and ribosome binding site </td>
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              <td>Tsu-Wang Sun </td>
 +
              <td>2451</td>
 +
            </tr>
 +
            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546006  BBa_K2546006 ]              </td>
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              <td>Coding </td>
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              <td>TonB + 2-haloacid dehalogenase + laccase </td>
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              <td>Tsu-Wang Sun </td>
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              <td>4721</td>
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            </tr>
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            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546007  BBa_K2546007 ]              </td>
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              <td>Plasmid_Backbone </td>
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              <td>cm 4657 </td>
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              <td>Tsu-Wang Sun </td>
 +
              <td>770</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546008  BBa_K2546008 ]              </td>
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              <td>Plasmid_Backbone </td>
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              <td>pME60C2 shuttle vector </td>
 +
              <td>Tsu-Wang Sun </td>
 +
              <td>5491</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546009  BBa_K2546009 ]              </td>
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              <td>Plasmid_Backbone </td>
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              <td>kan bbrf2 </td>
 +
              <td>Tsu-Wang Sun </td>
 +
              <td>4218</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="selectable">
 +
                [http://parts.igem.org/Part:BBa_K2546010  BBa_K2546010 ]              </td>
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              <td>Coding </td>
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              <td>TonB-dependent receptor (TonB) </td>
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              <td>Tsu-Wang Sun </td>
 +
              <td>2244</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="selectable">
 +
                [http://parts.igem.org/Part:BBa_K2546011  BBa_K2546011 ]              </td>
 +
              <td>Coding </td>
 +
              <td>Laccase (TLcc) </td>
 +
              <td>Tsu-Wang Sun </td>
 +
              <td>1584</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="selectable">
 +
                [http://parts.igem.org/Part:BBa_K2546012  BBa_K2546012 ]              </td>
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              <td>Coding </td>
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              <td>TonB with promoter and ribosome binding site </td>
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              <td>Yu-Hsi Lin </td>
 +
              <td>2305 </td>
 +
            </tr>
 +
         
 +
        </table>
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      </div>
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    </div>
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  </div>
 
{{NCHU_Taichung/footer}}
 
{{NCHU_Taichung/footer}}

Latest revision as of 01:57, 18 October 2018

NCHU_Taichung

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Parts Overview

Parts

Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene and replace the ori and also the resistance gene, which can also be seen in the basic part.

Basic Parts

  1. BBa_K2546001: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of halogenated compounds
  2. BBa_K2546004: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
  3. BBa_K2546007: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
  4. BBa_K2546008: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the Ori of pBBR1 and BBa_J364007 in pSB1C3.
  5. BBa_K2546010: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion carrier, siderophore, which is similar in structure with dioxin.
  6. BBa_K2546011: This part is coding for Laccase, which is related to lignin degradation.

Intermediate Parts

  1. BBa_K2546002: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding site.

Composite Parts

  1. BBa_K2546005: This part is the promoter circuit of BBa_K2546002.
  2. BBa_K2546006: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to let three gene be expressed in one circuit.
  3. BBa_K2546012: This part is the promoter circuit of BBa_K2546010.

Name Type Description Designer Length
BBa_K2546000 Coding dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf Tsu-Wang Sun
BBa_K2546001 Coding 2-haloacid dehalogenase(HAD) Tsu-Wang Sun 780
BBa_K2546002 Coding 2-haloacid dehalogenase plus laccase with robosome binding site Tsu-Wang Sun 2408
BBa_K2546003 Coding p+HL Tsu-Wang Sun 6
BBa_K2546004 Coding pME60C shuttle vector Tsu-Wang Sun 6489
BBa_K2546005 Coding 2-haloacid dehalogenase plus laccase with promoter and ribosome binding site Tsu-Wang Sun 2451
BBa_K2546006 Coding TonB + 2-haloacid dehalogenase + laccase Tsu-Wang Sun 4721
BBa_K2546007 Plasmid_Backbone cm 4657 Tsu-Wang Sun 770
BBa_K2546008 Plasmid_Backbone pME60C2 shuttle vector Tsu-Wang Sun 5491
BBa_K2546009 Plasmid_Backbone kan bbrf2 Tsu-Wang Sun 4218
BBa_K2546010 Coding TonB-dependent receptor (TonB) Tsu-Wang Sun 2244
BBa_K2546011 Coding Laccase (TLcc) Tsu-Wang Sun 1584
BBa_K2546012 Coding TonB with promoter and ribosome binding site Yu-Hsi Lin 2305