Difference between revisions of "Team:NCHU Taichung/Parts"

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{{NCHU_Taichung}}
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{{NCHU_Taichung/navbar}}
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<html>
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<div class="ui main text container">
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    <div class="ui segments" id="mainbox">
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      <div class="ui raised segment" id="mainbox">
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<div class="column full_size">
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        <h1 class="ui hidden header"></h1>
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<h1>Parts</h1>
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        <div class="ui huge header" id="top">Parts Overview</div>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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        <h2 class="ui header">Parts</h2>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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        <p class="longP">Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase
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</div>
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          DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part
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          of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene
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<div class="column full_size">
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          and replace the ori and also the resistance gene, which can also be seen in the basic part. </p>
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<div class="highlight decoration_background">
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        <h3 class="ui dividing header">Basic Parts</h3>
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<h3>Note</h3>
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        <ol class="ui ordered list">
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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          <li value="・">
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</div>
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            [http://parts.igem.org/Part:BBa_K2546001  BBa_K2546001:] This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of
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</div>
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            halogenated compounds</li>
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          <li value="・">
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<div class="clear extra_space"></div>
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            [http://parts.igem.org/Part:BBa_K2546004  BBa_K2546004:] This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
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<div class="line_divider"></div>
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            of pMP4657 and BBa_J364007 in pSB1C3. </li>
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<div class="clear extra_space"></div>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546007  BBa_K2546007:] This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori
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            of pMP4657 and BBa_J364007 in pSB1C3. </li>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546008  BBa_K2546008:] This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the
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            Ori of pBBR1 and BBa_J364007 in pSB1C3. </li>
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<div class="column two_thirds_size">
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          <li value="・">
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<div class="highlight decoration_B_full">
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            [http://parts.igem.org/Part:BBa_K2546010  BBa_K2546010:] This part is coding for TonB-dependent receptor, which can actively transport the ferric ion
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            carrier, siderophore, which is similar in structure with dioxin.</li>
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<h3>Adding parts to the registry</h3>
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          <li value="・">
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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            [http://parts.igem.org/Part:BBa_K2546011  BBa_K2546011:] This part is coding for Laccase, which is related to lignin degradation.</li>
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        </ol>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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        <h3 class="ui dividing header">Intermediate Parts</h3>
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<div class="button_link">
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        <ol class="ui ordered list">
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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          <li value="・">
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ADD PARTS
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            [http://parts.igem.org/Part:BBa_K2546002  BBa_K2546002:] This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding
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</a>
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            site.</li>
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</div>
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        </ol>
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        <h3 class="ui dividing header">Composite Parts</h3>
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</div>
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        <ol class="ui ordered list">
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</div>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546005  BBa_K2546005:] This part is the promoter circuit of BBa_K2546002.</li>
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          <li value="・">
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            [http://parts.igem.org/Part:BBa_K2546006  BBa_K2546006:] This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to
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<div class="column third_size">
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            let three gene be expressed in one circuit.</li>
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<div class="highlight decoration_A_full">
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          <li value="・">
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<h3>Inspiration</h3>
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            [http://parts.igem.org/Part:BBa_K2546012  BBa_K2546012:] This part is the promoter circuit of BBa_K2546010.</li>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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        </ol>
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        <h1 class="ui hidden header"></h1>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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        <table class="ui selectable teal celled small table">
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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            <tr>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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              <th class="three wide">Name</th>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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              <th class="three wide">Type</th>
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</ul>
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              <th class="four wide">Description</th>
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</div>
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              <th class="three wide">Designer</th>
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</div>
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              <th class="three wide">Length</th>
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            </tr>
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<div class="clear extra_space"></div>
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            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546000  BBa_K2546000 ]              </td>
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              <td>Coding </td>
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              <td>dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf </td>
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<div class="column full_size">
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              <td>Tsu-Wang Sun </td>
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              <td> </td>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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            </tr>
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<p>The information needed to initially create a part on the Registry is:</p>
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            <tr>
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<ul>
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              <td class="selectable">
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<li>Part Name</li>
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                [http://parts.igem.org/Part:BBa_K2546001  BBa_K2546001 ]              </td>
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<li>Part type</li>
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              <td>Coding </td>
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<li>Creator</li>
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              <td>2-haloacid dehalogenase(HAD) </td>
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<li>Sequence</li>
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              <td>Tsu-Wang Sun </td>
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<li>Short Description (60 characters on what the DNA does)</li>
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              <td>780</td>
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<li>Long Description (Longer description of what the DNA does)</li>
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            </tr>
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<li>Design considerations</li>
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            <tr>
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</ul>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546002  BBa_K2546002 ]              </td>
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<p>
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              <td>Coding </td>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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              <td>2-haloacid dehalogenase plus laccase with robosome binding site </td>
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              <td>Tsu-Wang Sun </td>
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</div>
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              <td>2408</td>
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            </tr>
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            <tr>
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<div class="clear extra_space"></div>
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              <td class="selectable">
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<div class="line_divider"></div>
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                [http://parts.igem.org/Part:BBa_K2546003  BBa_K2546003 ]              </td>
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<div class="clear extra_space"></div>
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              <td>Coding </td>
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              <td>p+HL </td>
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<div class="column full_size">
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              <td>Tsu-Wang Sun </td>
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<h3>Part Table </h3>
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              <td>6</td>
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            </tr>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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            <tr>
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              <td class="selectable">
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</html>
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                [http://parts.igem.org/Part:BBa_K2546004  BBa_K2546004 ]              </td>
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<groupparts>iGEM18 NCHU_Taichung</groupparts>
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              <td>Coding </td>
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<html>
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              <td>pME60C shuttle vector </td>
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</div>
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              <td>Tsu-Wang Sun </td>
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              <td>6489</td>
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            </tr>
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            <tr>
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              <td class="selectable">
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</html>
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                [http://parts.igem.org/Part:BBa_K2546005  BBa_K2546005 ]              </td>
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              <td>Coding </td>
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              <td>2-haloacid dehalogenase plus laccase with promoter and ribosome binding site </td>
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              <td>Tsu-Wang Sun </td>
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              <td>2451</td>
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            </tr>
 +
            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546006  BBa_K2546006 ]              </td>
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              <td>Coding </td>
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              <td>TonB + 2-haloacid dehalogenase + laccase </td>
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              <td>Tsu-Wang Sun </td>
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              <td>4721</td>
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            </tr>
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            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546007  BBa_K2546007 ]              </td>
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              <td>Plasmid_Backbone </td>
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              <td>cm 4657 </td>
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              <td>Tsu-Wang Sun </td>
 +
              <td>770</td>
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            </tr>
 +
            <tr>
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              <td class="selectable">
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                [http://parts.igem.org/Part:BBa_K2546008  BBa_K2546008 ]              </td>
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              <td>Plasmid_Backbone </td>
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              <td>pME60C2 shuttle vector </td>
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              <td>Tsu-Wang Sun </td>
 +
              <td>5491</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="selectable">
 +
                [http://parts.igem.org/Part:BBa_K2546009  BBa_K2546009 ]              </td>
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              <td>Plasmid_Backbone </td>
 +
              <td>kan bbrf2 </td>
 +
              <td>Tsu-Wang Sun </td>
 +
              <td>4218</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="selectable">
 +
                [http://parts.igem.org/Part:BBa_K2546010  BBa_K2546010 ]              </td>
 +
              <td>Coding </td>
 +
              <td>TonB-dependent receptor (TonB) </td>
 +
              <td>Tsu-Wang Sun </td>
 +
              <td>2244</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="selectable">
 +
                [http://parts.igem.org/Part:BBa_K2546011  BBa_K2546011 ]              </td>
 +
              <td>Coding </td>
 +
              <td>Laccase (TLcc) </td>
 +
              <td>Tsu-Wang Sun </td>
 +
              <td>1584</td>
 +
            </tr>
 +
            <tr>
 +
              <td class="selectable">
 +
                [http://parts.igem.org/Part:BBa_K2546012  BBa_K2546012 ]              </td>
 +
              <td>Coding </td>
 +
              <td>TonB with promoter and ribosome binding site </td>
 +
              <td>Yu-Hsi Lin </td>
 +
              <td>2305 </td>
 +
            </tr>
 +
         
 +
        </table>
 +
      </div>
 +
    </div>
 +
  </div>
 +
{{NCHU_Taichung/footer}}

Latest revision as of 01:57, 18 October 2018

NCHU_Taichung

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Parts Overview

Parts

Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene and replace the ori and also the resistance gene, which can also be seen in the basic part.

Basic Parts

  1. BBa_K2546001: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of halogenated compounds
  2. BBa_K2546004: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
  3. BBa_K2546007: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
  4. BBa_K2546008: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the Ori of pBBR1 and BBa_J364007 in pSB1C3.
  5. BBa_K2546010: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion carrier, siderophore, which is similar in structure with dioxin.
  6. BBa_K2546011: This part is coding for Laccase, which is related to lignin degradation.

Intermediate Parts

  1. BBa_K2546002: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding site.

Composite Parts

  1. BBa_K2546005: This part is the promoter circuit of BBa_K2546002.
  2. BBa_K2546006: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to let three gene be expressed in one circuit.
  3. BBa_K2546012: This part is the promoter circuit of BBa_K2546010.

Name Type Description Designer Length
BBa_K2546000 Coding dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf Tsu-Wang Sun
BBa_K2546001 Coding 2-haloacid dehalogenase(HAD) Tsu-Wang Sun 780
BBa_K2546002 Coding 2-haloacid dehalogenase plus laccase with robosome binding site Tsu-Wang Sun 2408
BBa_K2546003 Coding p+HL Tsu-Wang Sun 6
BBa_K2546004 Coding pME60C shuttle vector Tsu-Wang Sun 6489
BBa_K2546005 Coding 2-haloacid dehalogenase plus laccase with promoter and ribosome binding site Tsu-Wang Sun 2451
BBa_K2546006 Coding TonB + 2-haloacid dehalogenase + laccase Tsu-Wang Sun 4721
BBa_K2546007 Plasmid_Backbone cm 4657 Tsu-Wang Sun 770
BBa_K2546008 Plasmid_Backbone pME60C2 shuttle vector Tsu-Wang Sun 5491
BBa_K2546009 Plasmid_Backbone kan bbrf2 Tsu-Wang Sun 4218
BBa_K2546010 Coding TonB-dependent receptor (TonB) Tsu-Wang Sun 2244
BBa_K2546011 Coding Laccase (TLcc) Tsu-Wang Sun 1584
BBa_K2546012 Coding TonB with promoter and ribosome binding site Yu-Hsi Lin 2305