Difference between revisions of "Team:NCHU Taichung/Demonstrate"

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         <h2 class="ui dividing header">Endophyte Engineering</h2>
 
         <h2 class="ui dividing header">Endophyte Engineering</h2>
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         <p class="longP">During Vietnam War, the herbicide, Agent Orange was used by the U.S. military as a defoliant. Agent Orange contains
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         <p class="longP">We have successfully express genes in the endophyte using our engineered vector. See <a href="https://2018.igem.org/Team:NCHU_Taichung/Parts_Improved">Parts Improved</a> page,<a href="http://parts.igem.org/BBa_K2546004">BBa_K2546004</a> for more information. </p>
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          TCDD, which is the most toxic of the dioxins. The use of Agent Orange has left serious and long-term health impacts
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          upon the Vietnamese people and their offspring. </p>
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         <h2 class="ui dividing header">Dioxin Bioremediation</h2>
 
         <h2 class="ui dividing header">Dioxin Bioremediation</h2>
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         <p class="longP">During Vietnam War, the herbicide, Agent Orange was used by the U.S. military as a defoliant. Agent Orange contains
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         <p class="longP">The results of the recombinant bacteria growth test, show that the three genes code for the proteins HAD, TonB, Laccase functions. In our hypothesis, HAD catalyze dehalogenation degrade TCDD, so the HAD construct grew better in TCDD medium as expect. TonB construct grew worse in high concentration TCDD, just as we suppose that TonB intakes TCDD into the cell. The growth of strain TLcc declined in high TCDD concentration. It’s possible that the free radical attack the ring structure and cause damages to bacteria as shown in our results. Most importantly, the recombinant strain TonB+HAD+TLcc(J23100), which three genes on an open reading frame, had better growth with 0, 0.05, 0.1ppm TCDD treatment. This indicated that the toxicity effect of TCDD can be detoxified due to the synergistic functions of the three genes. </p>
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          TCDD, which is the most toxic of the dioxins. The use of Agent Orange has left serious and long-term health impacts
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          upon the Vietnamese people and their offspring. </p>
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           <img class="ui image clmnImg" src="https://static.igem.org/mediawiki/2018/8/8b/T--NCHU_Taichung--VETIVER3.png">
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           <img class="ui image clmnImg" src="https://static.igem.org/mediawiki/2018/9/9b/T--NCHU_Taichung--fig6.png">
 
          
 
          
 
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Revision as of 03:50, 18 October 2018

NCHU_Taichung

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Demonstrate

Endophyte Engineering

We have successfully express genes in the endophyte using our engineered vector. See Parts Improved page,BBa_K2546004 for more information.

Dioxin Bioremediation

The results of the recombinant bacteria growth test, show that the three genes code for the proteins HAD, TonB, Laccase functions. In our hypothesis, HAD catalyze dehalogenation degrade TCDD, so the HAD construct grew better in TCDD medium as expect. TonB construct grew worse in high concentration TCDD, just as we suppose that TonB intakes TCDD into the cell. The growth of strain TLcc declined in high TCDD concentration. It’s possible that the free radical attack the ring structure and cause damages to bacteria as shown in our results. Most importantly, the recombinant strain TonB+HAD+TLcc(J23100), which three genes on an open reading frame, had better growth with 0, 0.05, 0.1ppm TCDD treatment. This indicated that the toxicity effect of TCDD can be detoxified due to the synergistic functions of the three genes.