Parts

Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene and replace the ori and also the resistance gene, which can also be seen in the basic part.

Basic Parts

1. BBa_K2546001: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of halogenated compounds
2. BBa_K2546004: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
3. BBa_K2546007: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
4. BBa_K2546008: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the Ori of pBBR1 and BBa_J364007 in pSB1C3.
5. BBa_K2546010: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion carrier, siderophore, which is similar in structure with dioxin.
6. BBa_K2546011: This part is coding for Laccase, which is related to lignin degradation.

Intermediate Parts

1. BBa_K2546002: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding site.

Composite Parts

1. BBa_K2546005: This part is the promoter circuit of BBa_K2546002.
2. BBa_K2546006: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to let three gene be expressed in one circuit.
3. BBa_K2546012: This part is the promoter circuit of BBa_K2546010.

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Name	Type	Description	Designer	Length
BBa_K2546000	Coding	dfgdsfghfdsghfdsghgdsghgfdsghnfgdsgsf	Tsu-Wang Sun
BBa_K2546001	Coding	2-haloacid dehalogenase(HAD)	Tsu-Wang Sun	780
BBa_K2546002	Coding	2-haloacid dehalogenase plus laccase with robosome binding site	Tsu-Wang Sun	2408
BBa_K2546003	Coding	p+HL	Tsu-Wang Sun	6
BBa_K2546004	Coding	pME60C shuttle vector	Tsu-Wang Sun	6489
BBa_K2546005	Coding	2-haloacid dehalogenase plus laccase with promoter and ribosome binding site	Tsu-Wang Sun	2451
BBa_K2546006	Coding	TonB + 2-haloacid dehalogenase + laccase	Tsu-Wang Sun	4721
BBa_K2546007	Plasmid_Backbone	cm 4657	Tsu-Wang Sun	770
BBa_K2546008	Plasmid_Backbone	pME60C2 shuttle vector	Tsu-Wang Sun	5491
BBa_K2546009	Plasmid_Backbone	kan bbrf2	Tsu-Wang Sun	4218
BBa_K2546010	Coding	TonB-dependent receptor (TonB)	Tsu-Wang Sun	2244
BBa_K2546011	Coding	Laccase (TLcc)	Tsu-Wang Sun	1584
BBa_K2546012	Coding	TonB with promoter and ribosome binding site	Yu-Hsi Lin	2305